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Antimicrobial Agents and Chemotherapy, April 2008, p. 1519-1521, Vol. 52, No. 4
0066-4804/08/$08.00+0     doi:10.1128/AAC.01241-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Anti-BK Virus Activity of Nucleoside Analogs{triangledown}

Parmjeet Randhawa,1* Jiri Zemlicka,2 Andreas Sauerbrei,3 Chris Meier,4 Karl Y. Hostetler,5 James R. Beadle,5 Noush Afarin Farasati,1 Yuchen Huang,1 and Matthews Bradley5,6

Division of Transplantation Pathology, Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania,1 Developmental Therapeutics Program, Barbara Ann Karmanos Institute,2 Wayne State University School of Medicine, Detroit, Michigan 48201-1379; Institute of Virology and Antiviral Chemotherapy, University of Jena, Jena, Germany,3 Institute of Organic Chemistry, University of Hamburg, Hamburg, Germany,4 Department of Medicine, University of California, San Diego, La Jolla, California 92093-0676,5 Luitpold Pharmaceuticals, Annapolis, Maryland6

Received 21 September 2007/ Returned for modification 30 November 2007/ Accepted 20 December 2007


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ABSTRACT
 
Polyomavirus BK is an important pathogen in transplant recipients with no effective therapy. This study demonstrates that alkoxyalkyl esters of (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine and fatty acid derivatives of 9-[2-(phosphonomethyoxy)ethyl]adenine (P393 and P405) are potent and selective inhibitors of BK virus replication in vitro, with a 50% effective concentration in the micromolar-to-nanomolar range.


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TEXT
 
Polyomaviruses are widely latent DNA viruses, of which the most important species is BK virus (BKV). BKV is reactivated in 20 to 60% of renal transplant recipients, and nephropathy develops in up to 10% of them. BKV is also associated with hemorrhagic cystitis in up to 60% of bone marrow transplant patients (19, 26). No effective antiviral therapies are available. Although some medical centers have empirically used leflunomide and cidofovir, no proven clinical benefit has resulted (10, 23, 25).

We investigated the antiviral activities of several nucleoside analogs by using the BKV Gardner strain (ATCC VR837) grown in log-phase WI-38 cells (ATCC CCL-75) (7) in a 7-day quantitative PCR assay of viral replication. Toxicity was evaluated by the conventional neutral red assay and by quantifying the housekeeping gene for aspartoacylase. The technical details of these methods have been published previously (6, 19, 20). Selected chemical structures are depicted in Fig. 1 and 2, and the results of testing are summarized in Table 1.


Figure 1
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  		FIG. 1. Chemical structures of PMEA derivatives P393 and P405.


Figure 2
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  		FIG. 2. Methylenecyclopropane analogs of nucleoside analogs tested for anti-BKV activity.


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  		TABLE 1. Anti-BKV activities of selected nucleoside analogsa

Acyclic nucleoside phosphonates were tested because this class of compounds encompasses several clinically useful antiviral agents. In our system, 9-[2-(phosphonomethyoxy)-ethyl]adenine (PMEA) showed no significant activity (selectivity index [SI] = 1.3), confirming and extending prior work done with mouse polyomavirus (1). The prodrug form PMEA dipivoxil was approximately 2 logs more potent but showed only a marginal increase in selectivity (SI = 5.74). However, fatty acid derivatives of PMEA, namely, P393 and P405 (Fig. 1), showed striking activity. P393 exhibited a 50% cytotoxic concentration (CC50), a 50% effective concentration (EC50), and an SI of 165.3 ± 11.6 µM, 1.0 ± 0.31 µM, and 58.9, respectively. P405 had a comparable EC50 (2.3 ± 0.03 µM), but the CC50 was >100 µM and >200 µM in the first two experiments. By repeating the experiment in a concentration range of 100 to 1,000 µM, a more precise value of 528.6 µM was obtained and this generated an SI of 232.8. The mechanism by which fatty acid side chains enhance the efficacy of the parent compound was not determined. The possibility of increased transport into infected cells was considered, but one might have expected this to have resulted in lowering of the CC50, and this was not observed. Notably, while PMEA dipivoxil also increased cell permeability compared to the parent compound, as reflected by a lower EC50, it did not have the same selectivity as the PMEA derivatives.

(S)-9-(3-Hydroxy-2-phosphonylmethoxypropyl)-adenine [(S)-HPMPA] is the adenine analog of the broad-spectrum compound cidofovir. (S)-HPMPA is a broad-spectrum antiviral agent with demonstrated activity against orthopoxviruses, cytomegalovirus, human herpesvirus 6, adenovirus, and hepatitis B virus (2, 4, 9, 18, 21, 24). We have previously reported that the hexadecyloxypropyl (HDP) ester of cidofovir is very active against BKV in vitro (19). Our present work shows that the HDP ester of (S)-HPMPA is the most active compound tested to date, with a CC50, an EC50, and an SI of 0.8 ± 0.4 µM, 0.02 ± 0.006 µM, and 58.5. HDP-(S)-HPMPA is roughly nine times more active in vitro against BKV than HDP-cidofovir is (19). The parent compound (S)-HPMPA was recently reported not to be active against multiple strains of mouse and monkey polyomaviruses (11). In the latter study, HPMP derivatives containing a 5-azacytosine moiety were shown to have the best activity; the highest SI (58.3) was found for the compound hexadecyloxyethyl-cHPMP-5-azaC, with other related compounds showing SIs of <30.0.

There has been recent interest in profiling the antiviral activities of methylenecyclopropane analogs of nucleosides (Fig. 2) (27). The rationale is to introduce methylene groups, reduce the number of rotatable bonds, and increase the entropy factor, thereby altering the biologic properties of the compounds (28). Modification of acyclovir and ganciclovir on the basis of this principle has been used to generate a new class of antiherpesvirus compounds. However, in our assay, cyclopropavir, synguanol, synadenol, and ZSM-I-32-F showed no significant antiviral activity.

Nucleoside analogs that inhibit the human immunodeficiency virus enzyme reverse transcriptase were also tested (5). Several of these compounds are active against hepatitis B virus, a DNA virus with a life cycle that includes an RNA intermediate that is reverse transcribed back to the DNA genome (8, 12). We evaluated the anti-BKV activities of these compounds, which are already approved by the Food and Drug Administration. Didanosine, lamivudine, and stavudine were all found to be inactive at a screening concentration of 100 µM. Famciclovir and tenofovir disoproxil also showed no effect within the concentration limits imposed by the solubility of these compounds. Zidovudine (azidothymidine) was inactive.

The nucleoside analogs acyclovir and brivudine have previously been tested against several polyomavirus strains and found to have very low SIs (1). However, the synthesis of cycloSaligenyl (cycloSal)-nucleoside monophosphates results in compounds that have greatly increased activities against orthopoxviruses, herpesviruses, and human immunodeficiency virus (3, 13-16, 22). Hence, we tested cycloSal-nucleoside monophosphates of acyclovir and brivudine (5-H-cycloSal-acyclovir monophosphate and 3-methyl-cycloSal-3'-OH-bromovinyl deoxyuridine monophosphate) in our sys-tem but found no significant antiviral activity (Table 1). The failure of the cycloSal strategy for BKV is likely related to the fact that the small 5-kb genome encodes neither a thymidine kinase nor a DNA polymerase. The presumed increased intracellular uptake of cycloSal compounds with subsequent release of the nucleoside by chemical hydrolysis, therefore, did not translate into reduced BKV replication.

In conclusion, we tested several compounds for anti-BKV activity in vitro. The most active compound was HDP-(S)-HPMPA which had an EC50 of 0.02 µM and an SI of 58. The PMEA derivatives P393 and P403 were less active, with EC50s of 1.0 to 2.3 µM and SIs of 158 and 232, respectively. The other compounds lacked either efficacy or selectivity against BKV in vitro.

ADDENDUM IN PROOF Additional testing following acceptance of this manuscript showed that 2',3'-didehydro-3'-deoxythymidine has significant anti-BKV effect, with an SI, CC50, and EC50 of 79.2, 404.3 ± 49.3 µM, and 5.1 ± 1.9 µM, respectively. It is of interest that a related compound, 3'-fluoro-2'-deoxythymidine, is a potent inhibitor of adenovirus (17). Likewise, 3'-azido-thymidine is a well-known compound in clinical use for human immunodeficiency virus infection.


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ACKNOWLEDGMENTS
 
This work was supported by NIH grants AI51227 (P.R.), AI63360 (P.R.), AI66499 (M.B.), and CA32779 (J.Z.) and NIAID contract N01-AI-30044 (P.R.).


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FOOTNOTES
 
* Corresponding author. Mailing address: Division of Transplantation Pathology, Department of Pathology, University of Pittsburgh, Room E737, UPMC-Montefiore Hospital, 3459 Fifth Ave., Pittsburgh, PA 15213. Phone: (412) 647-7646. Fax: (412) 647-5237. E-mail: randhawapa{at}upmc.edu Back

{triangledown} Published ahead of print on 19 February 2008. Back


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Antimicrobial Agents and Chemotherapy, April 2008, p. 1519-1521, Vol. 52, No. 4
0066-4804/08/$08.00+0     doi:10.1128/AAC.01241-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.




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