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Antimicrobial Agents and Chemotherapy, September 2008, p. 3321-3326, Vol. 52, No. 9
0066-4804/08/$08.00+0     doi:10.1128/AAC.00379-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Efficacy of Quinoxaline-2-Carboxylate 1,4-Di-N-Oxide Derivatives in Experimental Tuberculosis

Esther Vicente,¹ Raquel Villar,¹ Asunción Burguete,¹ Beatriz Solano,¹ Silvia Pérez-Silanes,^1* Ignacio Aldana,¹ Joseph A. Maddry,³ Anne J. Lenaerts,⁴ Scott G. Franzblau,⁵ Sang-hyun Cho,⁵ Antonio Monge,¹ and Robert C. Goldman²

Unidad en Investigación y Desarrollo de Medicamentos, Centro de Investigación en Farmacobiología Aplicada (CIFA), Universidad de Navarra, C/ Irunlarrea s/n, 31080 Pamplona, Spain,¹ Therapeutics Research Program, Division of AIDS, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892,² Southern Research Institute, Birmingham, Alabama 35225-53053,³ Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523,⁴ Institute for Tuberculosis Research, University of Illinois at Chicago, Chicago, Illinois 60612⁵

Received 20 March 2008/ Returned for modification 2 May 2008/ Accepted 8 July 2008

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This study extends earlier reports regarding the in vitro efficacies of the 1,4-di-N-oxide quinoxaline derivatives against Mycobacterium tuberculosis and has led to the discovery of a derivative with in vivo efficacy in the mouse model of tuberculosis. Quinoxaline-2-carboxylate 1,4-di-N-oxide derivatives were tested in vitro against a broad panel of single-drug-resistant M. tuberculosis strains. The susceptibilities of these strains to some compounds were comparable to those of strain H₃₇Rv, as indicated by the ratios of MICs for resistant and nonresistant strains, supporting the premise that 1,4-di-N-oxide quinoxaline derivatives have a novel mode of action unrelated to those of the currently used antitubercular drugs. Specific derivatives were further evaluated in a series of in vivo assays, including evaluations of the maximum tolerated doses, the levels of oral bioavailability, and the efficacies in a low-dose aerosol model of tuberculosis in mice. One compound, ethyl 7-chloro-3-methylquinoxaline-2-carboxylate 1,4-dioxide, was found to be (i) active in reducing CFU counts in both the lungs and spleens of infected mice following oral administration, (ii) active against PA-824-resistant Mycobacterium bovis, indicating that the pathway of bioreduction/activation is different from that of PA-824 (a bioreduced nitroimidazole that is in clinical trials), and (iii) very active against nonreplicating bacteria adapted to low-oxygen conditions. These data indicate that 1,4-di-N-oxide quinoxalines hold promise for the treatment of tuberculosis.

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The high number of multidrug-resistant Mycobacterium tuberculosis strains circulating worldwide has increased concern that tuberculosis (TB) may once again become an incurable disease and has emphasized the need for new drugs to treat this infection. Over the last few years, extensively drug-resistant TB, defined as TB caused by M. tuberculosis strains resistant to isoniazid (INH), rifampin, any fluoroquinolone, and one of the injectable drugs amikacin, kanamycin, and capreomycin, has become a major concern for TB treatment and control in the global setting (2, 7, 9, 11, 12, 16, 19, 28, 31). In addition, strains with resistance to all of the major clinically used antitubercular drugs are known (21), thus answering the question posed by Dye and Espinal, "Will tuberculosis become resistant to all antibiotics?" (8).

Our antitubercular research group has previously published several papers in which the synthesis and primary biological valuation of a large number of quinoxaline and quinoxaline 1,4-di-N-oxide derivatives were described (14, 22, 24-27, 32). In this context, different quinoxaline-2-carboxylate 1,4-dioxide derivatives were synthesized and evaluated as anti-M. tuberculosis agents (15). Some of them showed good in vitro parameters in a cytotoxicity assay and in an M. tuberculosis-infected macrophage model (Table 1). In this report, we detail the in vitro activities and in vivo efficacies of quinoxaline-2-carboxylate 1,4-di-N-oxide derivatives.

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TABLE 1. Structures of the quinoxaline derivatives and previously reported in vitro activities against M. tuberculosisa

(Portions of these data were presented in abstract form at the Gordon Conference on Tuberculosis and Drug Development, Oxford, United Kingdom, August 2007, and the 38th Union World Conference on Lung Health of the International Union against Tuberculosis and Lung Disease, Cape Town, South Africa, November 2007.)

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Compound synthesis. The quinoxaline derivatives used in this study were prepared by procedures described in the literature (15, 26) by the reaction of the appropriate benzofuroxan with the corresponding β-ketoester. The structural properties of the compounds were confirmed by nuclear magnetic resonance, mass spectrometry, and infrared analyses, and the purity was established by elemental analyses. The compounds described in the present work have demonstrated activity in previous assays (15): a MIC and cytotoxicity assay and an assay of activity against M. tuberculosis growing in macrophages. A list of the compounds and their structures and biological activities is presented in Table 1.

Determination of minimum bactericidal concentrations (MBCs) and MICs for single-drug-resistant strains of M. tuberculosis. Compounds were dissolved in dimethyl sulfoxide and diluted in test media to the desired concentrations, with the final dimethyl sulfoxide concentration kept below 0.625%. MICs for a minimum of three strains of drug-resistant M. tuberculosis (each strain resistant to a single TB drug) were determined by the microplate Alamar Blue assay (MABA) (10).

The MBCs for the M. tuberculosis H₃₇Rv strain and the other strains tested were then determined by subculturing the strains onto drug-free solid medium and enumerating CFU after exposure for 5 days at 37°C in Middlebrook 7H9 medium supplemented with drug concentrations equivalent to and higher than the previously determined MICs for the respective strains. The MBC was the lowest concentration of the drug that killed more than 99.9% of the bacterial population present when the drug was added.

Potential for cross-resistance with PA-824. PA-824 is a nitroimidazole agent in clinical trials for treating TB. Mycobacterium bovis strains resistant to PA-824, created using Tn5367 mutagenesis (4), were obtained from Lacy Daniels (Texas A&M College of Pharmacy, Kingsville, TX). MICs were determined by broth microdilution using the MABA. PA-824 was obtained from the Global Alliance for Tuberculosis Drug Development (New York, NY).

Maximum tolerated dose (MTD) and oral bioavailability. All experiments using mice were approved by the Animal Care and Use Committee at Colorado State University (approval no. 06-221A-02; expiration date, 17 October 2008) and under Animal Welfare Assurance number A3572-01. Single doses of drugs at 100, 300, or 1,000 mg/kg of body weight were administered (by gavage) to three C57BL/6 female mice per dose. Mice were observed postadministration at 4 and 6 h and then twice daily for the duration of the study (1 week). The oral bioavailability was determined by bioassays as described previously (13).

Rapid in vivo screen. Eight- to 10-week-old female specific-pathogen-free C57BL/6-Ifngtm1ts mice (gamma interferon gene knockout [GKO] mice) were purchased from Jackson Laboratories, Bar Harbor, ME (6). Mice were infected via low-dose aerosol exposure to M. tuberculosis Erdman by using a Middlebrook aerosol generation device (Glas-Col Inc., Terre Haute, IN), and the rapid screening model was carried out as described previously (18). One day postinfection, three mice were sacrificed to verify the uptake of 50 to 100 CFU of bacteria per mouse. Negative control mice remained untreated. An INH-treated control group, receiving the drug via oral gavage at 25 mg/kg/day, was included in each study. Each treatment group consisted of five mice. Treatment was started 18 days postinfection and continued for nine consecutive days. Five infected mice were killed at the start of treatment as pretreatment controls. Drugs were administered daily by oral gavage using 0.5% methyl cellulose (200-µl volumes).

Dose-response analysis in the rapid in vivo screen. The in vivo activity of compound 8 (Tuberculosis Antimicrobial Acquisition and Coordinating Facility [TAACF] no. 151985) was examined in the rapid screening model to repeat the efficacy testing and to estimate the minimal effective dose. Doses of 25, 100, and 300 mg/kg were tested using the same methods as those in the initial in vivo test.

Statistical analysis. The viable counts were converted to logarithms, which were then evaluated by a one-way analysis of variance followed by a multiple-comparison analysis of variance using a one-way Tukey test (in the SigmaStat software program). Differences were considered significant at the 95% level of confidence.

Activity against nonreplicating persistent M. tuberculosis. The activity of compound 8 against nonreplicating M. tuberculosis was determined by measuring the activity against M. tuberculosis bacteria adapted to a low-oxygen environment under anaerobic conditions (3). This low-oxygen-recovery assay (LORA) quantifies antibacterial activity by measuring the ability of an M. tuberculosis recombinant strain, containing a plasmid with an acetamidase promoter driving a bacterial luciferase gene, to produce a luminescent signal when placed back into an environment with ambient oxygen.

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The most potent compounds from our previous studies were subjected to the following set of tests: determination of (i) the MICs for different single-drug-resistant strains of M. tuberculosis, (ii) the MBCs, (iii) the activities against nonreplicating bacteria, (iv) the MTDs, (v) the levels of oral bioavailability, and (vi) the in vivo efficacies.

Table 2 shows the MICs obtained for single-drug-resistant strains of M. tuberculosis, including those resistant to INH, rifampin, thiacetazone, ethambutol (EMB), ciprofloxacin (CIP), kanamycin (KAM), ethionamide, and p-aminosalicylic acid. In general, all the compounds showed good MICs for resistant strains; MICs ranged between 0.1 and 6.25 µg/ml, with the exception of that of 3,7-dimethylquinoxaline-2-carboxylate 1,4-di-N-oxide (compound 2), a drug which showed poor activity against EMB-resistant and KAM-resistant strains, with MICs of 25 µg/ml. The susceptibilities of resistant strains can be considered comparable to those of H₃₇Rv, as indicated by the ratios of MICs for resistant and nonresistant M. tuberculosis strains (calculations not shown). This finding suggests that there is minimal if any cross-resistance with the current anti-TB drugs, thereby supporting the notion of a novel mechanism of action. These results are promising for the development of new effective compounds against the growing number of drug-resistant strains. The quinoxaline-2-carboxylate derivative with a benzyl group in the carboxylate (compound 10) was the most active compound, with MICs of 0.4 µg/ml or less for the resistant strains.

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TABLE 2. MICs for strains of single-drug-resistant M. tuberculosis

Compound 7 is likely activated via bioreduction in bacteria, similar to the reduction observed for other N-oxides (29). Since PA-824, a nitroimidazole in clinical trials for treating TB, is bioreduced into an active intermediate (4, 5, 20), we tested the activity of compound 7 against an isogenic set of M. bovis strains with defined resistance to PA-824. PA-824 is bioreduced into an active form by a pathway involving the deazaflavin F₄₂₀ cofactor-dependent glucose dehydrogenase (Fdg1) and other cellular factors, including molybdopterin, a cofactor for many oxidoreductase enzymes. Thus, the loss of function of Fdg1 or the loss of the ability to synthesize the F₄₂₀ cofactor leads to resistance to PA-824 (Table 3). Compound 7 was active against all PA-824-resistant M. bovis strains tested, thus showing the lack of cross-resistance and supporting the evidence for a different pathway of drug activation. Niclosamide (an anthelmintic agent that shows poor absorption from the host intestine) was included as another control compound that is structurally different from PA-824 and activated by a different pathway (1).

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TABLE 3. In vitro activities against susceptible and genetically defined PA-824-resistant mycobacteria

Compound 7 was also tested under aerobic and anaerobic (LORA) conditions for activity against M. tuberculosis strain H₃₇Rv containing the luciferase reporter. Compound 7 was active under aerobic growth conditions (MIC = 0.24 µg/ml) and under anaerobic conditions (LORA MIC = 0.42 µg/ml). The corresponding values for PA-824 and niclosamide were as follows: MICs under aerobic growth conditions, 0.10 and 0.28 µg/ml, respectively, and LORA MICs, 1.4 and 0.32 µg/ml, respectively. Activity against nonreplicating bacteria is another unique property of quinoxaline 1,4-di-N-oxides. Although the idea is speculative, activity targeting nonreplicating bacteria may translate into faster sterilization of infected tissues, and we are in the process of testing this possibility in animal models. The long continuation phase for the treatment of TB is believed to be due in part to the presence of nonreplicating organisms that persist even in the presence of antitubercular drugs. PA-824 is an experimental nitroimidazole that is in phase I clinical trials. PA-824 is bioreduced by M. tuberculosis into an active component. Niclosamide is another bioreduced drug that is active in the LORA. Compound 7 was slightly more active than PA-824 in the LORA on a molar basis (1.5 µM versus 4.85 µM).

The MBCs of select compounds for H₃₇Rv, along with the MICs, were determined using the MABA (10). The MIC of compound 7 tested as 0.6 µg/ml in this assay, and the MBC was 2.5 µg/ml (MBC/MIC ratio = 4.2). The MIC of compound 6 was 0.2 µg/ml, with an MBC of 3.13 µg/ml (MBC/MIC ratio = 15.7), and the MIC of compound 10 was 0.39 µg/ml, with an MBC of >6.25 µg/ml (MBC/MIC ratio > 16.0). A compound is generally considered to be bactericidal if the ratio of the MBC to the MIC is 4 (30), so the ratios for these compounds against H₃₇Rv indicated bacteriostatic rather than bactericidal activity by this definition. However, bactericidal activity in vivo is determined by peak levels in blood and tissues and exposure times following oral administration. The in vivo peak levels of compound 7 in serum reached 5 to 8 µg/ml 15 to 30 min following the administration of 100- to 200-mg/kg doses, as determined by bioassays, and bactericidal activity was observed in vivo (see below for more on in vivo efficacy).

Selected compounds 6 to 12 were evaluated in in vivo assays, including tests for efficacy in the mouse model of TB infection. The MTD of each compound was determined by using an escalating single dose of the drug given to mice by oral gavage. No acute adverse effects, reactions, or toxicity were observed at oral doses of up to 1,000 mg/kg (the highest dose tested). Subsequent in vivo evaluations of the efficacies of compounds 6 to 12 were done with doses of 300 mg/kg administered via oral gavage to infected GKO C57BL/6 mice (18). Six of the seven compounds were found to be inactive in that they did not effectively reduce the bacterial numbers in the lungs and spleens with respect to those in the untreated controls (Table 4). While we have not definitively determined the reason for the lack of in vivo efficacy of compounds 6 and 8 to 12, it appears that the lack of activity of compound 10 may be due to extensive serum binding, since the MIC is reduced 200-fold in the presence of serum. Likewise, the lack of efficacy of compound 6 appears to be due to a short in vivo half-life, based on the results of preliminary studies. The lack of activity was not due to the potency of M. tuberculosis strain Erdman, as all of the compounds were active against this strain (Table 4).

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TABLE 4. Evaluation of in vivo efficacies in the GKO mouse modela

However, the ethyl 7-chloroquinoxaline derivative (compound 7) afforded significant reductions of 3.2 and 2.62 log₁₀ CFU in lungs and spleens, respectively. Generally, compounds are considered to be active if they yield at least a 0.75-log₁₀ reduction in bacterial counts compared to day 24 counts. The protection shown by compound 7 is similar to that afforded by clinically available compounds and other compounds classified as very active (17). INH reduced the numbers of CFU by 3.10 and 4.17 log units in the same experiment. Since the majority of compounds showing good in vitro activity do not show in vivo efficacy in mice (23), the in vivo activity of ethyl 7-chloro-3-methylquinoxaline-2-carboxylate 1,4-di-N-oxide is promising. Bactericidal activity of compound 7 was observed, since the final lung CFU count following 9 days of treatment was below the starting lung CFU count on day 15, the day therapy was initiated (6.81 log CFU untreated lung, compared to 4.55 log CFU following 9 days of treatment) (Table 4). Similar evidence for bactericidal activity was obtained from the spleen data, with an initial count of 5.37 log CFU untreated spleen compared to 3.95 log CFU following 9 days of treatment (Table 4).

In a second experiment (Table 5), the dose-response relationship for compound 7 in vivo was determined using the GKO mouse model and doses of 25, 100, and 300 mg/kg (Table 5). Compound 7 was weakly active in the lung and spleen at a dose of 25 mg/kg (CFU readings for tissues from treated mice on day 24 were lower than those for tissues from the untreated controls on day 24, but the activity appeared to be bacteriostatic). At 100 and 300 mg/kg (P, <0.001 by a one-way analysis of variance followed by a multiple-comparison analysis of variance using a one-way Tukey test in the SigmaStat software program), compound 7 appeared to be bactericidal, since the numbers of CFU recovered from both the lungs and spleens were well below the CFU levels observed on day 15 (Table 5), except for the spleens from the 100-mg/kg-dose group, in which the mean number of CFU was reduced by only 0.28 logs, which was not significant. Activity at a dose of 300 mg/kg was striking in that it lowered the numbers of CFU by 5.58 and 5.51 logs, respectively, in the lungs and spleens. Although no clear toxicity was apparent in the single-dose MTD test and in the first in vivo experiment, some toxicity was observed in the second dose-response experiment; three mice in the 300-mg/kg group died during treatment, which was truncated to 7 days instead of the usual 9 days. No CFU were recovered from the lung and spleen of one of the surviving mice dosed at 300 mg/kg when the entire organs were plated for CFU enumeration. Although it appears that multiple dosing at 300 mg/kg may result in some toxicity in infected animals, data collected to date on this series (quinoxaline-2-carboxylate 1,4-di-N-oxide derivatives) indicate that both in vitro (cytotoxicity) and in vivo toxicity can be separated from the antitubercular activity, as determined by plotting MICs versus 50% inhibitory concentrations for over 100 analogs, wherein no correlation was observed. Although the MTD is high (>1,000 mg/kg for normal mice), we have observed that some compounds can show toxicity in infected GKO mice at lower levels, due likely to the added stress of disease. Thus, 300 mg/kg may be on the borderline for tolerability in GKO infected mice and may therefore show toxicity in some experiments. Experiments with normal C57BL/6 mice and longer treatment times are being scheduled.

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TABLE 5. Dose-response data for compound 7 (TAACF 151985) in the GKO mouse model

In conclusion, an extended evaluation of the in vitro and in vivo antimycobacterial activities of quinoxaline 1,4-di-N-oxide derivatives was performed. Most of these compounds displayed good inhibitory activity against resistant strains, and only compound 2 was associated with apparent cross-resistance in EMB-resistant and KAM-resistant strains, although we have not yet confirmed these data. Compound 7 was the only analog active in vivo. This activity was clearly due to adequate oral bioavailability and levels in the blood (measured at 3, 6, and 9 µg/ml for the 25-, 100-, and 300-mg/kg doses, respectively) and to a terminal elimination half-life of 4 to 8 h (unpublished data). Finally, we did detect bactericidal activity when we tested the sera of animals dosed orally with 200 mg of compound 7/kg and bled at 30 min. Sera diluted twofold killed 2.51 logs, and when diluted fourfold, they still killed 1.15 logs after 7 days of incubation. Thus, compound 7 has all of the necessary characteristics of a validated lead that can serve as the starting point for more advancement in medicinal chemical and preclinical development.

 
Antimycobacterial data were provided by the TAACF through research and development contracts.

This research was funded in part by contracts N01-AI-95364 (Southern Research Institute/University of Illinois at Chicago) and N01-AI-95385 (Colorado State University) from the National Institute of Allergy and Infectious Diseases (NIAID).

 
* Corresponding author. Mailing address: Unidad en Investigación y Desarrollo de Medicamentos, Centro de Investigación en Farmacobiología Aplicada (CIFA), Universidad de Navarra, C/ Irunlarrea s/n, 31080 Pamplona, Spain. Phone: 34 948 425653. Fax: 34 948 425652. E-mail: sperez{at}unav.es

Published ahead of print on 14 July 2008.

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Antimicrobial Agents and Chemotherapy, September 2008, p. 3321-3326, Vol. 52, No. 9
0066-4804/08/$08.00+0     doi:10.1128/AAC.00379-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

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