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Antimicrobial Agents and Chemotherapy, April 2009, p. 1673-1678, Vol. 53, No. 4
0066-4804/09/$08.00+0     doi:10.1128/AAC.01340-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Mutations in Three Distinct Loci Cause Resistance to Peptide Deformylase Inhibitors in Bacillus subtilis

Yann Duroc, Carmela Giglione, and Thierry Meinnel^*

Protein Maturation, Cell Fate and Therapeutics, ISV, UPR2355, Centre National de la Recherche Scientifique, Bât. 23A, 1 avenue de la Terrasse, F-91198 Gif-sur-Yvette Cedex, France

Received 7 October 2008/ Returned for modification 11 December 2008/ Accepted 20 January 2009

Top ABSTRACT INTRODUCTION REFERENCES

 
Bacillus subtilis mutants with resistance against peptide deformylase inhibitors were isolated. All showed a bypass of the pathway through mutations in three genes required for formylation of Met-tRNA^fMet, fmt, folD, and glyA. glyA corresponds to a yet uncharacterized locus inducing resistance. The bypass of formylation caused robust fitness reduction but was not accompanied by alterations of the transcription profile. A subtle adaptation of the enzymes of the intermediary metabolism was observed.

Top ABSTRACT INTRODUCTION REFERENCES

 
In bacteria, all newly synthesized polypeptides transiently carry a formylated N terminus (1, 18, 33). Peptide deformylase (PDF) catalyzes the subsequent removal of the formyl group, and its gene is essential (11, 22, 23). The natural compound actinonin was the first PDF inhibitor (PDFI) characterized (4, 6, 8). Mechanisms causing PDFI resistance involve (i) mutations in the target gene, (ii) bypassing of the formylation pathway, or (iii) efflux of PDFI (4, 9, 10, 15, 17, 19, 20, 25, 31). Bacillus subtilis has two functional PDF genes, def and ykrB (14). The mechanisms of resistance have been studied only in bacteria expressing one active PDF gene. No analysis has approached the implications beyond the fitness cost often associated with PDFI resistance.

To isolate variants resistant to PDFI, 100 µl of an exponential-phase B. subtilis culture was plated onto Mueller-Hinton (MH) agar supplemented with 12 µg/ml of actinonin. The plates were incubated for 2 days at 37°C, after which resistant colonies were restreaked and isolated. Cells were also plated on minimal medium (MM) agar broth (15 mM ammonium sulfate, 80 mM K₂HPO₄, 45 mM KH₂PO₄, 3.5 mM sodium citrate, 800 µM MgSO₄, 0.5% [wt/vol] glucose) supplemented with tryptophan (50 mg/liter) and glycine (25 mg/liter) where indicated below. To assess the fitness cost, growth rates were measured in different broths at 37°C. Cells (10⁶) were inoculated into 10 ml of MH medium (Fluka) or in MM without glucose and supplemented with 0.5% (wt/vol) of the indicated carbon source, and then the optical density at 600 nm was measured. In order to identify mutations in open reading frames and/or promoters, given gene loci were amplified with specific primers as shown in Table 1, and the sequences were determined.

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TABLE 1. Mutations, resistance levels, and fitness costs of actinonin-resistant strains

The resistance of B. subtilis 168 to actinonin was challenged on MH agar at four times the MIC. The resistance rate (10^–7) was stable, as repeated streaking on drug-free media did not promote loss of resistance. Resistant strains grew at concentrations much higher than four times the MIC (Table 1). Actinonin-resistant mutants did not show any cross-resistance to other antibiotics but were resistant to other classes of PDFI (7). Both open reading frame and promoter regions of genes (def, ykrB, fmt, and folD), the alteration or loss of function of which causes actinonin resistance in other bacteria, were sequenced. No mutation was retrieved in def and ykrB, and 80% were located in fmt (Table 1). Two-thirds of these mutations led to protein sequence alterations, with large deletions due to premature stops, and promoted loss of function of fmt. The fmt-114 strain featured a 114-codon deletion. Single changes involved the catalytic mechanism or binding of the substrates (Table 1; Fig. 1A and B).

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FIG. 1. Structural impact of substitutions leading to PDFI resistance in B. subtilis. (A) Structural view of E. coli formylmethionine transferase complexed with formyl methionyl-tRNAfMet (Protein Data Bank code, 2fmt). The enzyme is represented as a yellow ribbon, formylmethionine as pink solid bonds, and tRNAfMet as blue solid bonds. E. coli residues corresponding to mutated B. subtilis residues are in red. The inset shows the sequence alignment of the formylmethionine transferase active sites from E. coli and B. subtilis. Mutated residues are in red. (B through D) Structural view of human methylenetetrahydrofolate dehydrogenase/cyclohydrolase (Protein Data Bank code, 1a4i). The enzyme is represented as a yellow ribbon, the active/binding sites as pink ribbons, and NADP as blue solid bonds. Mutated or deleted residues are in red. (B) NADP binding site and deleted residues downstream. (C) THF binding site and deleted residues. (D) Mutation on Ala18 in the human equivalent of E17Q in B. subtilis. (E) Sequence alignment of methylenetetrahydrofolate dehydrogenase/cyclohydrolase from Homo sapiens and B. subtilis. Mutated or deleted residues are in red. Residues that are conserved between the two sequences are in bold. Residues involved in the THF binding site are marked with an asterisk, residues implied in the NADP binding site are marked with a filled circle, and residues implied in dimer formation are marked with a filled triangle. (F) Structural view of serine hydroxymethyltransferase from Bacillus stearothermophilus in complex with pyridoxal-5'-phosphate (Protein Data Bank code, 1kkj). The enzyme is represented as a yellow ribbon, and pyridoxal-5'-phosphate is represented with blue solid bonds. The pyridoxal-5'-phosphate binding site is in pink. A B. stearothermophilus residue corresponding to a residue deleted in B. subtilis is in red.

Other mutations were located in folD (Table 1). folD encodes 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase, which produces 10-formyl-tetrahydrofolate (THF), the donor of N-formyl to Met-tRNA^fMet. Similar mutations have been described only for Salmonella enterica (24). The loss of function of folD not only bypasses PDF function but also inactivates pathways that use 10-formyl-THF (Fig. 2). When folD is inactivated, the strain cannot grow on MM. None of the resistant strains with a folD alteration could grow on MM, indicating that the mutations induced loss of function. Several mutations corresponded to deletions. The first deletion identified included residues 67 to 70, with modifications of residues 71 to 73. Structural interpretation was based on the three-dimensional model of FolD (29). The THF binding site is composed of residues conserved in both humans (2) and B. subtilis (Fig. 1C to E). The substitutions modify the position of the THF binding site, leading to a dramatic decrease in the reaction efficiency. The second deletion identified (residues 172 to 175) is located next to the NADP binding site ₁₆₅GRSNIVG₁₇₁ (₁₇₂GRSKIVG₁₇₈ in humans) (Fig. 1B to E).

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FIG. 2. Translation initiation in bacteria, Met-tRNA formylation, ribosome-mediated protein synthesis, and bypass of the pathway in PDFI-resistant bacteria. tRNAi, tRNAfMet.

There was only one strain carrying an fmt mutation that could not grow on MM (Table 1). Given that a nonmutated FolD protein occurred, the origin of the impairment should be due to a mutation upstream in the pathway. One reaction is catalyzed by the glyA product, serine hydroxymethyltransferase (Fig. 2), which produces glycine and 5,10-methylene-THF, the substrate of FolD. As growth of the mutant strain on MM was restored in the presence of glycine, this favored an involvement of glyA. A deletion of codon 224 in glyA was identified. According to the crystallographic model (32), residue 224 lies within the pyridoxal 5'-phosphate (PLP) binding site encompassing residues Thr223 to Lys226 (Fig. 1F). All serine hydroxymethyltransferases have five Thr residues near the active site Lys226, which form the internal glycine-aldimine with PLP (3, 32). Deletion of Thr224 alters PLP binding (Fig. 1F), inactivating the enzyme. The combination of both the glyA and fmt mutations confers a higher resistance than that of the strain carrying only the fmt mutation. The MIC of actinonin for the first strain was increased 10-fold (Table 1), indicating that (i) the Arg116Cys substitution does not completely inactivate fmt and (ii) GlyA modification further completes the bypass of formylation.

All aforementioned mutations inducing PDFI resistance caused fitness costs with two- to threefold reductions in the growth rate in rich media (Table 1). This decrease was higher in MM. This finding confirms that formylation is needed for rapid growth in bacteria (5, 13, 16, 25, 28). However, the fitness cost was less pronounced than in E. coli (4, 13, 16). Strains with unambiguous inactivation of the fmt gene had distinct doubling times (Table 1). We investigated whether compensatory mechanisms could improve the growth rate by serially passaging several strains in nonselective media through 50 generations. Unlike with PDFI-resistant gram-negative bacteria (12, 21, 25), we were unable to show any improvement in the doubling time. Thus, the fitness burden induced by PDFI resistance is robust. Sequencing of the three B. subtilis tRNA^fMet genes revealed no modifications or rearrangement, unlike for various gram-positive bacteria (12, 25, 30). Sequencing of the IF₂ gene (infB) did not reveal any mutation, although altered expression contributes to fitness cost reduction in Pseudomonas aeruginosa (30). Two-dimensional gel electrophoresis of protein accumulation in the fmt-114 mutant revealed patterns different from those for the wild type (WT), indicative of adaptation mechanisms associated with formylation bypass. Comparison of the transcriptome of the fmt-114 strain with that of the WT revealed that the expression of a small number of genes was altered by more than twofold, with 308 upregulated and 25 downregulated (Fig. 3A).

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FIG. 3. Physiological differences between WT and PDFI-resistant strains. (A) Gene categories to which the 333 significantly regulated genes belong. Custom compare analysis of the complete B. subtilis transcriptome of the WT and resistant strains was performed on microarrays at Eurogentech (Herstaing, Belgium). (B) The doubling times of the WT and fmt-114 strain were studied in MH medium or MM supplemented with the indicated carbon source. The fitness cost corresponds to the ratio of the doubling time of the WT with that of the resistant derivative. TCA, tricarboxylic acid.

To confirm the expression changes between the WT and the fmt-114 strain, quantitative PCR experiments were performed, using rplL for normalization (Table 2). Total RNA was isolated from 5-ml portions of bacterial samples by using an RNeasy minikit (Qiagen) as described by the manufacturer. Genomic DNA was eliminated by RNase-free DNase I treatment during the isolation procedure. cDNAs were prepared, using the SuperScript III First-Strand system. We subjected 5 µg of total RNA to reverse transcription, using random hexamers. The cDNA was then amplified by PCR, using specific oligonucleotides. Real-time PCR was optimized with a LightCycler FastStart DNA Master Sybr green kit (Roche) for each primer pair shown in Table 3 (the amplification efficiency was always >90%), using a standard cDNA. Each cDNA sample was independently quantified three times, with two technical replicates of each. Relative transcript levels were calculated by using the relative expression software tool (REST) (27). Expression of the rplL gene was used as a reference for the determination of induction levels. No evidence for regulation was observed for genes involved in translation. The gene categories showing the highest changes corresponded to genes encoding functions of the intermediary metabolism (Fig. 3A). These categories produce the most-abundant proteins, which suggests that energetic metabolism limits growth and is the root of the fitness cost. Thus, in the tested conditions, (i) there is no global change of gene expression and (ii) the adaptation mechanisms involved are subtle.

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TABLE 2. Absolute gene regulation in the fmt-114 mutant

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TABLE 3. Nucleotide sequences of the primers used for PCR amplification

To understand whether those adaptation mechanisms could be mediated by the growth rate and/or substrate utilization, we tested if fitness could depend on the growth medium. Growth was performed on either MH medium or MM. The growth rate with several carbon sources varied from 18.6 to 53.1 min. There was no correlation between growth rate and fitness cost, and two types of fitness defects could be detected (Fig. 3B). In rich medium or in MM supplemented with a mono- or disaccharide, the doubling time was reduced by twofold. With carboxylic acids such as pyruvate, the growth rate was reduced fourfold. We concluded that sugar utilization in the context of the fmt-114 strain is more effective than that of carboxylic acids. This difference reflects the limited efficiency of the tricarboxylic cycle/oxidative phosphorylation and the pentose phosphate pathway in the context of the mutant strain, possibly compensating for the overall reduced translation efficiency of these major proteins induced by the loss of formylation of Met-tRNA^fMet.

 
Y.D. was supported by Centre National de la Recherche Scientifique (CNRS, France) and by a postdoctoral grant from the Région Ile-de-France. This work was supported by CNRS and by grant ANR-06-MIME-010 from the Agence Nationale de la Recherche (ANR, France).

 
* Corresponding author. Mailing address: Protein Maturation, Cell Fate and Therapeutics, ISV, UPR2355, Centre National de la Recherche Scientifique, Bât. 23A, 1 avenue de la Terrasse, F-91198 Gif-sur-Yvette Cedex, France. Phone: 33169823612. Fax: 33169823607. E-mail: thierry.meinnel{at}isv.cnrs-gif.fr

Published ahead of print on 26 January 2009.

Top ABSTRACT INTRODUCTION REFERENCES

 

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Antimicrobial Agents and Chemotherapy, April 2009, p. 1673-1678, Vol. 53, No. 4
0066-4804/09/$08.00+0     doi:10.1128/AAC.01340-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services E-mail this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Duroc, Y. Articles by Meinnel, T. Search for Related Content PubMed PubMed Citation Articles by Duroc, Y. Articles by Meinnel, T.