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Antimicrob Agents Chemother. 1982 May; 21(5): 844-845

Effect of dihydrostreptomycin on active transport in isolated bacterial membrane vesicles.

R G Eagon, R J Hartle, J B Rake and S Abdel-Sayed

ABSTRACT

Membrane vesicles prepared from bacterial cells grown in the absence of dihydrostreptomycin but subsequently incubated in the presence of dihydrostreptomycin transported proline normally, but vesicles prepared from cells grown in media to which dihydrostreptomycin was added 30 min before harvesting had a greatly impaired ability to accumulate proline. The latter cells extruded protons normally but were unable to maintain a proton gradient as effectively as normal cells. These data indicated that metabolism was required for dihydrostreptomycin to exert an effect on the bacterial cell membrane.

Antimicrob Agents Chemother. 1982 May; 21(5): 844-845






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