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Antimicrob Agents Chemother. 1973 February; 3(2): 194-197
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Spectrophotometric Method for the Estimation of 6-Aminopenicillanic Acid

Khadija Shaikh, P. G. Talati and D. M. Gang

Sarabhai Research Centre, Baroda, India

ABSTRACT

A simple, rapid, and sensitive method is described whereby 6-aminopenicillanic acid can be spectrophotometrically determined in the presence of penicillins and their degradation products without prior separation. D-(+)-Glucosamine is used as reagent. The effect of such parameters as pH, temperature, and time of heating on the formation of the chromophore is described. The recommended range is from 25 to 250 µg of 6-aminopenicillanic acid.

Antimicrob Agents Chemother. 1973 February; 3(2): 194-197
Copyright © 1973 American Society for Microbiology. All Rights Reserved.





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