This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rao, U R Articles by Vickery, A C Search for Related Content PubMed PubMed Citation Articles by Rao, U R Articles by Vickery, A C

 Previous Article  |  Next Article 

Antimicrob Agents Chemother. 1991 November; 35(11): 2219-2224

Brugia malayi and Acanthocheilonema viteae: antifilarial activity of transglutaminase inhibitors in vitro.

College of Public Health, University of South Florida, Tampa 33612.

ABSTRACT

The possible involvement of transglutaminase-catalyzed reactions in survival of adult worms, microfilariae (mf), and infective larvae of the filarial parasite Brugia malayi was studied in vitro by using the specific pseudosubstrate monodansylcadaverine (MDC) and the active-site inhibitors cystamine or iodoacetamide. These inhibitors significantly inhibited parasite mobility in a dose-dependent manner. This inhibition was associated with irreversible biochemical lesions followed by filarial death. A structurally related, inactive analog of MDC, dimethyldansylcadaverine, did not affect the mobility or survival of the parasites. Adult worms failed to release mf when they were incubated in the presence of MDC or cystamine, and this inhibitory effect on mf release was concentration dependent. Similar embryostatic and macrofilaricidal effects of MDC were observed in Acanthocheilonema viteae adult worms. These studies suggest that transglutaminase-catalyzed reactions may play an important role in the growth, development, and survival of filarial parasites.

This article has been cited by other articles:

• Chen, R., Gao, B., Huang, C., Olsen, B., Rotundo, R. F., Blumenstock, F., Saba, T. M. (2000). Transglutaminase-mediated fibronectin multimerization in lung endothelial matrix in response to TNF-alpha. Am. J. Physiol. Lung Cell. Mol. Physiol. 279: L161-L174 [Abstract] [Full Text]