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Antimicrobial Agents and Chemotherapy, Jul 1995, 1596-1598, Vol 39, No. 7
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Susceptibility of Acanthamoeba castellanii to contact lens disinfecting solutions

S Zanetti, PL Fiori, A Pinna, S Usai, F Carta and G Fadda
Institute of Microbiology and Virology, University of Sassari, Italy.

A corneal isolate of Acanthamoeba castellanii was exposed to commercial contact lens disinfecting solutions containing hydrogen peroxide, benzalkonium chloride, polyaminopropyl biguanide, polyquaternium 1, and chlorhexidine-thimerosal. The minimum trophozoite amebicidal concentration and exposure times required to kill trophozoites and cysts were determined. Solutions containing hydrogen peroxide or chlorhexidine-thimerosal were active against both trophozoites and cysts. The benzalkonium chloride-based solution was effective only against trophozoites. Solutions containing polyaminopropyl biguanide or polyquaternium 1 were completely ineffective. The need for adequate exposure times must be stressed.


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