This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Liao, X. Articles by Hancock, R. E. Search for Related Content PubMed PubMed Citation Articles by Liao, X. Articles by Hancock, R. E.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, Aug 1995, 1871-1874, Vol 39, No. 8
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Cloning and characterization of the Pseudomonas aeruginosa pbpB gene encoding penicillin-binding protein 3

X Liao and RE Hancock
Department of Microbiology & Immunology, University of British Columbia, Vancouver, Canada.

Clones containing the pbpB gene which encodes penicillin-binding protein (PBP) 3 of Pseudomonas aeruginosa were detected by hybridization by PCR amplification with primers based on the conserved sequences of high-molecular-weight PBPs. The translated amino acid sequence demonstrated 45% identity and had a total of 66% conserved amino acids relative to the Escherichia coli PBP3. The pbpB gene was located upstream of a gene homologous to the E. coli murE gene, which encodes uridine diphosphate-N-acetyl muramic acid-tripeptide synthetase. The overexpressed pbpB gene product reacted with 3H- penicillin G and had an apparent molecular weight of 60,000.

This article has been cited by other articles:

• Legaree, B. A., Adams, C. B., Clarke, A. J. (2007). Overproduction of Penicillin-Binding Protein 2 and Its Inactive Variants Causes Morphological Changes and Lysis in Escherichia coli. J. Bacteriol. 189: 4975-4983 [Abstract] [Full Text]  
• Legaree, B. A., Daniels, K., Weadge, J. T., Cockburn, D., Clarke, A. J. (2007). Function of penicillin-binding protein 2 in viability and morphology of Pseudomonas aeruginosa. J Antimicrob Chemother 59: 411-424 [Abstract] [Full Text]  
• Aguilar, C., Friscina, A., Devescovi, G., Kojic, M., Venturi, V. (2003). Identification of Quorum-Sensing-Regulated Genes of Burkholderia cepacia. J. Bacteriol. 185: 6456-6462 [Abstract] [Full Text]  
• Inui, T., Endo, T., Matsushita, T. (2000). Morphological Changes and Lysis Induced by beta -Lactams Associated with the Characteristic Profiles of Affinities of Penicillin-Binding Proteins in Actinobacillus pleuropneumoniae. Antimicrob. Agents Chemother. 44: 1518-1523 [Abstract] [Full Text]  
• Hao, J., Kendrick, K. E. (1998). Visualization of Penicillin-Binding Proteins during Sporulation of Streptomyces griseus. J. Bacteriol. 180: 2125-2132 [Abstract] [Full Text]