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Antimicrobial Agents and Chemotherapy, Sep 1995, 2128-2131, Vol 39, No. 9
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Resistance of Candida albicans biofilms to antifungal agents in vitro

SP Hawser and LJ Douglas
Laboratory of Microbiology, University of Glasgow, United Kingdom.

Biofilms formed by Candida albicans on small discs of catheter material were resistant to the action of five clinically important antifungal agents as determined by [3H]leucine incorporation and tetrazolium reduction assays. Fluconazole showed the greatest activity, and amphotericin B showed the least activity against biofilm cells. These findings were confirmed by scanning electron microscopy of the biofilms.


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