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Antimicrobial Agents and Chemotherapy, 11 1996, 2651-2654, Vol 40, No. 11
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Quantitation of intracellular zidovudine phosphates by use of combined cartridge-radioimmunoassay methodology

BL Robbins, BH Waibel and A Fridland
Department of Infectious Disease, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.

This report describes the development of a potentially clinical method to measure the cellular metabolites of zidovudine (ZDV) in patients receiving the drug. This new method combines the use of Sep-Pak cartridges to separate ZDV phosphates with radioimmunoassaying to quantitate ZDV. The detection limit is 0.02 pmol/10(6) cells, and this assay can measure a wide range of intracellular drug concentrations. The use of the cartridge-radioimmunoassay methodology should prove very useful for in vivo cellular pharmacokinetic studies of ZDV.


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