This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Henze, U. U.
Right arrow Articles by Berger-Bachi, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Henze, U. U.
Right arrow Articles by Berger-Bachi, B.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, 09 1996, 2121-2125, Vol 40, No. 9
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Penicillin-binding protein 4 overproduction increases beta-lactam resistance in Staphylococcus aureus

UU Henze and B Berger-Bachi
Institute of Medical Microbiology, University of Zurich, Switzerland.

The Staphylococcus aureus mutant strain PVI selected in vitro for methicillin resistance overexpressed penicillin-binding protein (PBP) 4. In the wild-type parent strain the pbp4 gene was separated by 419 nucleotides from a divergently transcribed abcA locus coding for an ATP- binding cassette transporter. The mutant PVI was shown to have a deletion in the pbp4-abcA promoter region that affected pbp4 transcription but not expression of abcA. Introduction of the pbp4 gene plus the mutant promoter region into different genetic backgrounds revealed that PBP 4 overproduction was sufficient to increase in vitro- acquired methicillin resistance independently of other chromosomal genes. The role of the AbcA transporter in methicillin resistance remained unknown.


This article has been cited by other articles:

  • Truong-Bolduc, Q. C., Hooper, D. C. (2007). The Transcriptional Regulators NorG and MgrA Modulate Resistance to both Quinolones and {beta}-Lactams in Staphylococcus aureus. J. Bacteriol. 189: 2996-3005 [Abstract] [Full Text]  
  • Riordan, J. T., Muthaiyan, A., Van Voorhies, W., Price, C. T., Graham, J. E., Wilkinson, B. J., Gustafson, J. E. (2007). Response of Staphylococcus aureus to Salicylate Challenge. J. Bacteriol. 189: 220-227 [Abstract] [Full Text]  
  • Fuller, E., Elmer, C., Nattress, F., Ellis, R., Horne, G., Cook, P., Fawcett, T. (2005). {beta}-Lactam Resistance in Staphylococcus aureus Cells That Do Not Require a Cell Wall for Integrity. Antimicrob. Agents Chemother. 49: 5075-5080 [Abstract] [Full Text]  
  • Wolter, D. J., Hanson, N. D., Lister, P. D. (2005). AmpC and OprD Are Not Involved in the Mechanism of Imipenem Hypersusceptibility among Pseudomonas aeruginosa Isolates Overexpressing the mexCD-oprJ Efflux Pump. Antimicrob. Agents Chemother. 49: 4763-4766 [Abstract] [Full Text]  
  • Darini, A. L. d. C., Palazzo, I. C. V., Felten, A. (2004). Cefoxitin Does Not Induce Production of Penicillin Binding Protein 2a in Methicillin-Susceptible Staphylococcus aureus Strains. J. Clin. Microbiol. 42: 4412-4413 [Full Text]  
  • Reipert, A., Ehlert, K., Kast, T., Bierbaum, G. (2003). Morphological and Genetic Differences in Two Isogenic Staphylococcus aureus Strains with Decreased Susceptibilities to Vancomycin. Antimicrob. Agents Chemother. 47: 568-576 [Abstract] [Full Text]  
  • Bamberger, D. M., Herndon, B. L., Fitch, J., Florkowski, A., Parkhurst, V. (2002). Effects of Neutrophils on Cefazolin Activity and Penicillin-Binding Proteins in Staphylococcus aureus Abscesses. Antimicrob. Agents Chemother. 46: 2878-2884 [Abstract] [Full Text]  
  • Felten, A., Grandry, B., Lagrange, P. H., Casin, I. (2002). Evaluation of Three Techniques for Detection of Low-Level Methicillin-Resistant Staphylococcus aureus (MRSA): a Disk Diffusion Method with Cefoxitin and Moxalactam, the Vitek 2 System, and the MRSA-Screen Latex Agglutination Test. J. Clin. Microbiol. 40: 2766-2771 [Abstract] [Full Text]  
  • Finan, J. E., Archer, G. L., Pucci, M. J., Climo, M. W. (2001). Role of Penicillin-Binding Protein 4 in Expression of Vancomycin Resistance among Clinical Isolates of Oxacillin-Resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 45: 3070-3075 [Abstract] [Full Text]  
  • Schrader-Fischer, G., Berger-Bächi, B. (2001). The AbcA Transporter of Staphylococcus aureus Affects Cell Autolysis. Antimicrob. Agents Chemother. 45: 407-412 [Abstract] [Full Text]  
  • Sieradzki, K., Pinho, M. G., Tomasz, A. (1999). Inactivated pbp4 in Highly Glycopeptide-resistant Laboratory Mutants of Staphylococcus aureus. J. Biol. Chem. 274: 18942-18946 [Abstract] [Full Text]  
  • Petersson, A. C., Kamme, C., Miörner, H. (1999). Disk with High Oxacillin Content Discriminates between Methicillin-Resistant and Borderline Methicillin-Susceptible Staphylococcus aureus Strains in Disk Diffusion Assays Using a Low Salt Concentration. J. Clin. Microbiol. 37: 2047-2050 [Abstract] [Full Text]  
  • Cavassini, M., Wenger, A., Jaton, K., Blanc, D. S., Bille, J. (1999). Evaluation of MRSA-Screen, a Simple Anti-PBP 2a Slide Latex Agglutination Kit, for Rapid Detection of Methicillin Resistance in Staphylococcus aureus. J. Clin. Microbiol. 37: 1591-1594 [Abstract] [Full Text]  
  • Skov, R. L., Pallesen, L. V., Poulsen, R. L., Espersen, F. (1999). Evaluation of a new 3-h hybridization method for detecting the mecA gene in Staphylococcus aureus and comparison with existing genotypic and phenotypic susceptibilty testing methods. J Antimicrob Chemother 43: 467-475 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»