This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vrany, J. D.
Right arrow Articles by Suci, P. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vrany, J. D.
Right arrow Articles by Suci, P. A.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, Jun 1997, 1352-1358, Vol 41, No. 6
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Comparison of recalcitrance to ciprofloxacin and levofloxacin exhibited by Pseudomonas aeruginosa bofilms displaying rapid-transport characteristics

JD Vrany, PS Stewart and PA Suci
Center for Biofilm Engineering, Montana State University, Bozeman 59717, USA.

Attenuated total reflection Fourier transform infrared spectroscopy was used to measure transport of the fluoroquinolones (FQs) ciprofloxacin and levofloxacin into Pseudomonas aeruginosa biofilms. Biofilms were exposed to each FQ at dose levels of 100, 250, and 500 microg/ml for 30 min. A mathematical transport model was used to extract the diffusion coefficient, binding site density, and adsorption and desorption rates for each experiment. Recalcitrance of the biofilms toward each FQ was evaluated by comparison of efficacies with planktonic bacteria. By this criterion, biofilms were found to exhibit more recalcitrance toward levofloxacin than ciprofloxacin under the experimental conditions. These results cannot be explained by the more hindered transport of levofloxacin, implicating the domination of physiological factors.


This article has been cited by other articles:

  • Walters, M. C. III, Roe, F., Bugnicourt, A., Franklin, M. J., Stewart, P. S. (2003). Contributions of Antibiotic Penetration, Oxygen Limitation, and Low Metabolic Activity to Tolerance of Pseudomonas aeruginosa Biofilms to Ciprofloxacin and Tobramycin. Antimicrob. Agents Chemother. 47: 317-323 [Abstract] [Full Text]  
  • Martinez, J. L., Baquero, F. (2002). Interactions among Strategies Associated with Bacterial Infection: Pathogenicity, Epidemicity, and Antibiotic Resistance. Clin. Microbiol. Rev. 15: 647-679 [Abstract] [Full Text]  
  • Spoering, A. L., Lewis, K. (2001). Biofilms and Planktonic Cells of Pseudomonas aeruginosa Have Similar Resistance to Killing by Antimicrobials. J. Bacteriol. 183: 6746-6751 [Abstract] [Full Text]  
  • Lewis, K. (2001). Riddle of Biofilm Resistance. Antimicrob. Agents Chemother. 45: 999-1007 [Full Text]  
  • Anderl, J. N., Franklin, M. J., Stewart, P. S. (2000). Role of Antibiotic Penetration Limitation in Klebsiella pneumoniae Biofilm Resistance to Ampicillin and Ciprofloxacin. Antimicrob. Agents Chemother. 44: 1818-1824 [Abstract] [Full Text]  
  • Costerton, J. W., Stewart, P. S., Greenberg, E. P. (1999). Bacterial Biofilms: A Common Cause of Persistent Infections. Science 284: 1318-1322 [Abstract] [Full Text]  
  • Ishida, H., Ishida, Y., Kurosaka, Y., Otani, T., Sato, K., Kobayashi, H. (1998). In Vitro and In Vivo Activities of Levofloxacin against Biofilm-Producing Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 42: 1641-1645 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»