This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Peyron, F.
Right arrow Articles by Regli, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Peyron, F.
Right arrow Articles by Regli, P.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, Jul 1997, 1537-1540, Vol 41, No. 7
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Evaluation of a flow cytofluorometric method for rapid determination of amphotericin B susceptibility of yeast isolates

F Peyron, A Favel, H Guiraud-Dauriac, M El Mzibri, C Chastin, G Dumenil and P Regli
Laboratoire de Botanique, Cryptogamie, et Biologie Cellulaire (PAON, EA 864), Faculte de Pharmacie, Universite de la Mediterranee, Marseille, France.

A rapid-flow cytofluorometric susceptibility test for in vitro amphotericin B testing of yeasts was evaluated and compared to the National Committee for Clinical Laboratory Standards (NCCLS) M27-T reference broth macrodilution method. The flow cytofluorometric method is based on the detection of decreased green fluorescence intensity of cells stained with DiOC5(3), a membrane potential-sensitive cationic dye, after drug treatment. Testing was performed on 134 clinical isolates (Candida spp. and Torulopsis glabrata). From the dose-response curve obtained for each isolate, three endpoints were calculated by computer analysis (the concentrations at which the fluorescence intensity was reduced by 50, 80, and 90%, i.e., 50% inhibitory concentration [IC50], IC80, and IC90, respectively). A regression analysis correlating these endpoints with the M27-T MICs showed that the best agreement was obtained with IC80. The flow cytofluorometric method showed good reproducibility with control strains. These initial results suggest that the flow cytofluorometric method is a valid alternative to the NCCLS reference method.


This article has been cited by other articles:

  • Florent, M., Noel, T., Ruprich-Robert, G., Da Silva, B., Fitton-Ouhabi, V., Chastin, C., Papon, N., Chapeland-Leclerc, F. (2009). Nonsense and Missense Mutations in FCY2 and FCY1 Genes Are Responsible for Flucytosine Resistance and Flucytosine-Fluconazole Cross-Resistance in Clinical Isolates of Candida lusitaniae. Antimicrob. Agents Chemother. 53: 2982-2990 [Abstract] [Full Text]  
  • Chaturvedi, V., Ramani, R., Pfaller, M. A. (2004). Collaborative Study of the NCCLS and Flow Cytometry Methods for Antifungal Susceptibility Testing of Candida albicans. J. Clin. Microbiol. 42: 2249-2251 [Abstract] [Full Text]  
  • Viejo-Diaz, M., Andres, M. T., Fierro, J. F. (2004). Modulation of In Vitro Fungicidal Activity of Human Lactoferrin against Candida albicans by Extracellular Cation Concentration and Target Cell Metabolic Activity. Antimicrob. Agents Chemother. 48: 1242-1248 [Abstract] [Full Text]  
  • Liu, Y., Tortora, G., Ryan, M. E., Lee, H.-M., Golub, L. M. (2002). Potato Dextrose Agar Antifungal Susceptibility Testing for Yeasts and Molds: Evaluation of Phosphate Effect on Antifungal Activity of CMT-3. Antimicrob. Agents Chemother. 46: 1455-1461 [Abstract] [Full Text]  
  • Peyron, F., Favel, A., Calaf, R., Michel-Nguyen, A., Bonaly, R., Coulon, J. (2002). Sterol and Fatty Acid Composition of Candida lusitaniae Clinical Isolates. Antimicrob. Agents Chemother. 46: 531-533 [Abstract] [Full Text]  
  • Francois, F., Noel, T., Pepin, R., Brulfert, A., Chastin, C., Favel, A., Villard, J. (2001). Alternative Identification Test Relying upon Sexual Reproductive Abilities of Candidalusitaniae Strains Isolated from Hospitalized Patients. J. Clin. Microbiol. 39: 3906-3914 [Abstract] [Full Text]  
  • Rex, J. H., Pfaller, M. A., Walsh, T. J., Chaturvedi, V., Espinel-Ingroff, A., Ghannoum, M. A., Gosey, L. L., Odds, F. C., Rinaldi, M. G., Sheehan, D. J., Warnock, D. W. (2001). Antifungal Susceptibility Testing: Practical Aspects and Current Challenges. Clin. Microbiol. Rev. 14: 643-658 [Abstract] [Full Text]  
  • Peyron, F., Favel, A., Michel-Nguyen, A., Gilly, M., Regli, P., Bolmström, A. (2001). Improved Detection of Amphotericin B-Resistant Isolates of Candida lusitaniae by Etest. J. Clin. Microbiol. 39: 339-342 [Abstract] [Full Text]  
  • Ramani, R., Chaturvedi, V. (2000). Flow Cytometry Antifungal Susceptibility Testing of Pathogenic Yeasts other than Candida albicans and Comparison with the NCCLS Broth Microdilution Test. Antimicrob. Agents Chemother. 44: 2752-2758 [Abstract] [Full Text]  
  • Alvarez-Barrientos, A., Arroyo, J., Canton, R., Nombela, C., Sanchez-Perez, M. (2000). Applications of Flow Cytometry to Clinical Microbiology. Clin. Microbiol. Rev. 13: 167-195 [Abstract] [Full Text]  
  • Chang Chang, H., Jung Chang, J., Huey Huang, A., Chain Chang, T. (2000). Evaluation of a Capacitance Method for Direct Antifungal Susceptibility Testing of Yeasts in Positive Blood Cultures. J. Clin. Microbiol. 38: 971-976 [Abstract] [Full Text]  
  • Koshlukova, S. E., Lloyd, T. L., Araujo, M. W. B., Edgerton, M. (1999). Salivary Histatin 5 Induces Non-lytic Release of ATP from Candida albicans Leading to Cell Death. J. Biol. Chem. 274: 18872-18879 [Abstract] [Full Text]  
  • Di Giorgio, C., Faraut-Gambarelli, F., Imbert, A., Minodier, P., Gasquet, M., Dumon, H. (1999). Flow cytometric assessment of amphotericin B susceptibility in Leishmania infantumisolates from patients with visceral leishmaniasis. J Antimicrob Chemother 44: 71-76 [Abstract] [Full Text]  
  • Favel, A., Peyron, F., De Meo, M., Michel-Nguyen, A., Carriere, J., Chastin, C., Regli, P. (1999). Amphotericin B susceptibility testing of Candida lusitaniae isolates by flow cytofluorometry: comparison with the Etest and the NCCLS broth macrodilution method. J Antimicrob Chemother 43: 227-232 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»