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Antimicrobial Agents and Chemotherapy, Jul 1997, 1575-1578, Vol 41, No. 7
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Effect of granulocyte colony-stimulating factor on the candidacidal activity of polymorphonuclear neutrophils and their collaboration with fluconazole

U Natarajan, E Brummer and DA Stevens
Department of Medicine, Santa Clara Valley Medical Center, and California Institute for Medical Research, San Jose 95128-2699, USA.

The effect of granulocyte colony-stimulating factor (GCSF) treatment of polymorphonuclear neutrophils (PMN) in vitro was studied with respect to their candidacidal activity. The candidacidal activity of PMN was found to be significantly increased when they were pretreated with GCSF. Fluconazole (1 microg/ml) was found to be highly fungistatic (90%) for Candida albicans Sh27 and collaborated with PMN for significantly increased killing. Collaborative killing by PMN significantly increased when they were treated with GCSF before and after fungal exposure. The enhancing activities of GCSF required optimization of the GCSF dose and were thus inoculum and strain dependent.

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