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Antimicrobial Agents and Chemotherapy, October 1998, p. 2557-2563, Vol. 42, No. 10
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Characterization of In40 of Enterobacter aerogenes BM2688, a Class 1 Integron with Two New Gene Cassettes, cmlA2 and qacF

Marie-Cécile Ploy,1,2 Patrice Courvalin,1 and Thierry Lambert1,3,*

Unité des Agents Antibactériens, Institut Pasteur, 75724 Paris Cedex 15,1 Laboratoire de Bactériologie-Virologie-Hygiène, CHU Dupuytren, 87000 Limoges,2 and Centre d'Etudes Pharmaceutiques, 92296 Châtenay-Malabry,3 France

Received 11 March 1998/Returned for modification 26 May 1998/Accepted 21 July 1998

Enterobacter aerogenes BM2688, which is resistant to multiple antibiotics, and its aminoglycoside-susceptible derivative BM2688-1 were isolated from the same clinical sample. Strain BM2688 harbored plasmid pIP833, which carries a class 1 integron, In40, containing (in addition to qacEDelta 1 and sul1, which are characteristic of class 1 integrons) four gene cassettes: aac(6')-Ib, qacF, cmlA2, and oxa-9. The cmlA2 gene had 83.7% identity with the previously described nonenzymatic chloramphenicol resistance cmlA1 gene. The qacF gene conferred resistance to quaternary ammonium compounds and displayed a high degree of similarity with qacE (67.8% identity) which, however, has been found as part of a cassette with a very different 59-base element. The oxa-9 gene was not expressed due to a lack of promoter sequences. Study of the antibiotic-susceptible derivative BM2688-1 indicated that a 3,148-bp deletion between the 3' end of the aac(6')-Ib gene and the 3' conserved segment of In40 was responsible for the loss of resistance. The occurrence of this DNA rearrangement, which did not involve homologous sequences, suggests that the In40 integrase could promote recombination at secondary sites.

* Corresponding author. Mailing address: Unité des Agents Antibactériens, 28 rue du Docteur Roux, Institut Pasteur, 75724 Paris Cedex 15, France. Phone: (33) (1) 45 68 83 21. Fax: (33) (1) 45 68 83 19. E-mail: pcourval{at}pasteur.fr.

Antimicrobial Agents and Chemotherapy, October 1998, p. 2557-2563, Vol. 42, No. 10
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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