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Antimicrobial Agents and Chemotherapy, November 1998, p. 3006-3008, Vol. 42, No. 11
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Pulsed-Exposure and Postantibiotic Leukocyte Enhancement Effects of Amikacin, Clarithromycin, Clofazimine, and Rifampin against Intracellular Mycobacterium avium

Lionel Horgen, Arnaud Jerome, and Nalin Rastogi*

Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, Morne Jolivière, BP 484, 97165 Pointe-à-Pitre, Guadeloupe

Received 16 June 1998/Returned for modification 4 August 1998/Accepted 1 September 1998

We investigated the postantibiotic effects (PAEs) of four agents against Mycobacterium avium in a human macrophage model under two different experimental conditions. For postantibiotic leukocyte enhancement (PALE), bacteria were exposed to antibiotics prior to their phagocytosis, whereas for pulsed exposure (PE), antibiotics were added after phagocytosis. In both cases, the drugs were used at their peak concentrations in serum (Cmax) for 2 h. The results showed two different patterns: one for the drug for which results under PE and PALE test conditions did not significantly differ (amikacin) and one for drugs for which PAE values were significantly higher under PE test conditions (clarithromycin, clofazimine, and rifampin). These data suggest that even a brief exposure of M. avium to peak concentrations of certain drugs in serum may result in prolonged and persistent suppression of bacterial growth inside human macrophages.

* Corresponding author. Mailing address: Institut Pasteur, Morne Jolivière, BP 484, F-97165 Pointe-à-Pitre Cedex, Guadeloupe. Phone: 590-893-881. Fax: 590-893-880. E-mail: rastogi{at}ipagua.gp.

Antimicrobial Agents and Chemotherapy, November 1998, p. 3006-3008, Vol. 42, No. 11
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.




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