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Antimicrobial Agents and Chemotherapy, February 1998, p. 444-446, Vol. 42, No. 2
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The Cys607right-arrow Tyr Change in the UL97 Phosphotransferase Confers Ganciclovir Resistance to Two Human Cytomegalovirus Strains Recovered from Two Immunocompromised Patients

Fausto Baldanti,1 Mark R. Underwood,2 Christine L. Talarico,2 Lavinia Simoncini,1 Antonella Sarasini,1 Karen K. Biron,2 and Giuseppe Gerna1,*

Servizio di Virologia, IRCCS Policlinico S. Matteo, 27100 Pavia, Italy,1 and Department of Virology, Glaxo Wellcome Inc., Research Triangle Park, North Carolina 277092

Received 6 June 1997/Returned for modification 2 October 1997/Accepted 5 November 1997

Two ganciclovir (GCV)-resistant human cytomegalovirus (HCMV) strains recovered from an AIDS patient (strain VR4990) and a heart transplant recipient (strain VR5474) showed a Cys607right-arrowTyr change in the UL97-encoded phosphotransferase. No amino acid substitutions were observed in the viral DNA polymerase. Marker transfer experiments showed marked reduction in GCV phosphorylation and drug susceptibility of the recombinant HCMV strain VR4990rec2-1-1. These results further extend the region of the carboxy-terminal domain of the UL97 phosphotransferase involved in GCV substrate recognition.

* Corresponding author. Mailing address: Servizio di Virologia, IRCCS Policlinico S. Matteo, 27100 Pavia, Italy. Phone: 39-382-502644. Fax: 39-382-502599 and 39-382-423320. E-mail: virology{at}ipv36.unipv.it.

Antimicrobial Agents and Chemotherapy, February 1998, p. 444-446, Vol. 42, No. 2
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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