This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pitout, J. D. D.
Right arrow Articles by Sanders, C. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pitout, J. D. D.
Right arrow Articles by Sanders, C. C.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, March 1998, p. 596-600, Vol. 42, No. 3
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Plasmid-Mediated Resistance to Expanded-Spectrum Cephalosporins among Enterobacter aerogenes Strains

Johann D. D. Pitout,1,dagger Kenneth S. Thomson,1,* Nancy D. Hanson,1 Anton F. Ehrhardt,1 Philip Coudron,2 and Christine C. Sanders1

Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, Nebraska 68178,1 and Department of Pathology, Hunter Holmes McGuire Medical Center, Richmond, Virginia 232492

Received 12 May 1997/Returned for modification 9 September 1997/Accepted 17 December 1997

Resistance to expanded-spectrum cephalosporins commonly develops in Enterobacter aerogenes during therapy due to selection of mutants producing high levels of the chromosomal Bush group 1 beta -lactamase. Recently, resistant strains producing plasmid-mediated extended-spectrum beta -lactamases (ESBLs) have been isolated as well. A study was designed to investigate ESBL production among 31 clinical isolates of E. aerogenes from Richmond, Va., with decreased susceptibility to expanded-spectrum cephalosporins and a positive double-disk potentiation test. Antibiotic susceptibility was determined by standard disk diffusion and agar dilution procedures. beta -Lactamases were investigated by an isoelectric focusing overlay technique which simultaneously determined isoelectric points (pIs) and substrate or inhibitor profiles. Decreased susceptibility to cefotaxime, ceftazidime, and aztreonam (MIC range, 1 to 64 µg/ml) was detected and associated with resistance to gentamicin and trimethoprim-sulfamethoxazole. All strains produced an inducible Bush group 1 beta -lactamase (pI 8.3). Twenty-nine of the 31 isolates also produced an enzyme similar to SHV-4 (pI 7.8), while 1 isolate each produced an enzyme similar to SHV-3 (pI 6.9) and to SHV-5 (pI 8.2). The three different SHV-derived ESBLs were transferred by transconjugation to Escherichia coli C600N and amplified by PCR. Plasmid profiles of the clinical isolates showed a variety of different large plasmids. Because of the linkage of resistance to aminoglycosides and trimethoprim-sulfamethoxazole with ESBL production, it is possible that the usage of these drugs was responsible for selecting plasmid-mediated resistance to extended-spectrum cephalosporins in E. aerogenes. Furthermore, it is important that strains such as these be recognized, because they can be responsible for institutional spread of resistance genes.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE 68178. Phone: (402) 280-1881. Fax: (402) 280-1225. E-mail: kstaac{at}creighton.edu.

dagger Present address: Department of Medical Microbiology, University of the Orange Free State, Bloemfontein, South Africa 9300.

Antimicrobial Agents and Chemotherapy, March 1998, p. 596-600, Vol. 42, No. 3
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • al Naiemi, N., Bart, A., de Jong, M. D., Vandenbroucke-Grauls, C. M., Rietra, P. J. G. M., Debets-Ossenkopp, Y. J., Wever, P. C., Spanjaard, L., Bos, A. J., Duim, B. (2006). Widely Distributed and Predominant CTX-M Extended-Spectrum {beta}-Lactamases in Amsterdam, The Netherlands.. J. Clin. Microbiol. 44: 3012-3014 [Abstract] [Full Text]  
  • Paterson, D. L., Bonomo, R. A. (2005). Extended-Spectrum {beta}-Lactamases: a Clinical Update. Clin. Microbiol. Rev. 18: 657-686 [Abstract] [Full Text]  
  • Pitout, J. D. D., Gregson, D. B., Church, D. L., Elsayed, S., Laupland, K. B. (2005). Community-Wide Outbreaks of Clonally Related CTX-M-14 {beta}-Lactamase-Producing Escherichia coli Strains in the Calgary Health Region. J. Clin. Microbiol. 43: 2844-2849 [Abstract] [Full Text]  
  • Kim, J., Lim, Y.-M. (2005). Prevalence of Derepressed AmpC Mutants and Extended-Spectrum {beta}-Lactamase Producers among Clinical Isolates of Citrobacter freundii, Enterobacter spp., and Serratia marcescens in Korea: Dissemination of CTX-M-3, TEM-52, and SHV-12. J. Clin. Microbiol. 43: 2452-2455 [Abstract] [Full Text]  
  • Schlesinger, J., Navon-Venezia, S., Chmelnitsky, I., Hammer-Munz, O., Leavitt, A., Gold, H. S., Schwaber, M. J., Carmeli, Y. (2005). Extended-Spectrum Beta-Lactamases among Enterobacter Isolates Obtained in Tel Aviv, Israel. Antimicrob. Agents Chemother. 49: 1150-1156 [Abstract] [Full Text]  
  • Jones, L. A., McIver, C. J., Kim, M.-J., Rawlinson, W. D., White, P. A. (2005). The aadB Gene Cassette Is Associated with blaSHV Genes in Klebsiella Species Producing Extended-Spectrum {beta}-Lactamases. Antimicrob. Agents Chemother. 49: 794-797 [Abstract] [Full Text]  
  • Pai, H., Hong, J. Y., Byeon, J.-H., Kim, Y.-K., Lee, H.-J. (2004). High Prevalence of Extended-Spectrum {beta}-Lactamase-Producing Strains among Blood Isolates of Enterobacter spp. Collected in a Tertiary Hospital during an 8-Year Period and Their Antimicrobial Susceptibility Patterns. Antimicrob. Agents Chemother. 48: 3159-3161 [Abstract] [Full Text]  
  • Jeong, S. H., Bae, I. K., Lee, J. H., Sohn, S. G., Kang, G. H., Jeon, G. J., Kim, Y. H., Jeong, B. C., Lee, S. H. (2004). Molecular Characterization of Extended-Spectrum Beta-Lactamases Produced by Clinical Isolates of Klebsiella pneumoniae and Escherichia coli from a Korean Nationwide Survey. J. Clin. Microbiol. 42: 2902-2906 [Abstract] [Full Text]  
  • Mulvey, M. R., Bryce, E., Boyd, D., Ofner-Agostini, M., Christianson, S., Simor, A. E., Paton, S. (2004). Ambler Class A Extended-Spectrum Beta-Lactamase-Producing Escherichia coli and Klebsiella spp. in Canadian Hospitals. Antimicrob. Agents Chemother. 48: 1204-1214 [Abstract] [Full Text]  
  • Bell, J. M., Turnidge, J. D., Jones, R. N., the SENTRY Asia-Pacific Participants,, (2003). Prevalence of Extended-Spectrum {beta}-Lactamase-Producing Enterobacter cloacae in the Asia-Pacific Region: Results from the SENTRY Antimicrobial Surveillance Program, 1998 to 2001. Antimicrob. Agents Chemother. 47: 3989-3993 [Abstract] [Full Text]  
  • Pitout, J. D. D., Reisbig, M. D., Mulvey, M., Chui, L., Louie, M., Crowe, L., Church, D. L., Elsayed, S., Gregson, D., Ahmed, R., Tilley, P., Hanson, N. D. (2003). Association between Handling of Pet Treats and Infection with Salmonella enterica Serotype Newport Expressing the AmpC {beta}-Lactamase, CMY-2. J. Clin. Microbiol. 41: 4578-4582 [Abstract] [Full Text]  
  • Pitout, J. D. D., Reisbig, M. D., Venter, E. C., Church, D. L., Hanson, N. D. (2003). Modification of the Double-Disk Test for Detection of Enterobacteriaceae Producing Extended-Spectrum and AmpC {beta}-Lactamases. J. Clin. Microbiol. 41: 3933-3935 [Abstract] [Full Text]  
  • Lee, S. H., Kim, J. Y., Shin, S. H., An, Y. J., Choi, Y. W., Jung, Y. C., Jung, H. I., Sohn, E. S., Jeong, S. H., Lee, K. J. (2003). Dissemination of SHV-12 and Characterization of New AmpC-Type Beta-Lactamase Genes among Clinical Isolates of Enterobacter Species in Korea. J. Clin. Microbiol. 41: 2477-2482 [Abstract] [Full Text]  
  • Aibinu, I. E., Ohaegbulam, V. C., Adenipekun, E. A., Ogunsola, F. T., Odugbemi, T. O., Mee, B. J. (2003). Extended-Spectrum {beta}-Lactamase Enzymes in Clinical Isolates of Enterobacter Species from Lagos, Nigeria. J. Clin. Microbiol. 41: 2197-2200 [Abstract] [Full Text]  
  • Chollet, R., Bollet, C., Chevalier, J., Mallea, M., Pages, J.-M., Davin-Regli, A. (2002). mar Operon Involved in Multidrug Resistance of Enterobacter aerogenes. Antimicrob. Agents Chemother. 46: 1093-1097 [Abstract] [Full Text]  
  • Canton, R., Oliver, A., Coque, T. M., Varela, M. d. C., Perez-Diaz, J. C., Baquero, F. (2002). Epidemiology of Extended-Spectrum {beta}-Lactamase-Producing Enterobacter Isolates in a Spanish Hospital during a 12-Year Period. J. Clin. Microbiol. 40: 1237-1243 [Abstract] [Full Text]  
  • Qiang, Y. Z., Qin, T., Fu, W., Cheng, W. P., Li, Y. S., Yi, G. (2002). Use of a rapid mismatch PCR method to detect gyrA and parC mutations in ciprofloxacin-resistant clinical isolates of Escherichia coli. J Antimicrob Chemother 49: 549-552 [Abstract] [Full Text]  
  • Mammeri, H., Laurans, G., Eveillard, M., Castelain, S., Eb, F. (2001). Coexistence of SHV-4- and TEM-24-Producing Enterobacter aerogenes Strains before a Large Outbreak of TEM-24-Producing Strains in a French Hospital. J. Clin. Microbiol. 39: 2184-2190 [Abstract] [Full Text]  
  • De Gheldre, Y., Struelens, M. J., Glupczynski, Y., De Mol, P., Maes, N., Nonhoff, C., Chetoui, H., Sion, C., Ronveaux, O., Vaneechoutte, M. (2001). National Epidemiologic Surveys of Enterobacter aerogenes in Belgian Hospitals from 1996 to 1998. J. Clin. Microbiol. 39: 889-896 [Abstract] [Full Text]  
  • Villa, L., Pezzella, C., Tosini, F., Visca, P., Petrucca, A., Carattoli, A. (2000). Multiple-Antibiotic Resistance Mediated by Structurally Related IncL/M Plasmids Carrying an Extended-Spectrum beta -Lactamase Gene and a Class 1 Integron. Antimicrob. Agents Chemother. 44: 2911-2914 [Abstract] [Full Text]  
  • Gottlieb, T., Wolfson, C. (2000). Comparison of the MICs of cefepime for extended-spectrum {beta}-lactamase-producing and non-extended-spectrum {beta}-lactamase-producing strains of Enterobacter cloacae. J Antimicrob Chemother 46: 330-332 [Full Text]  
  • Bornet, C., Davin-Regli, A., Bosi, C., Pages, J.-M., Bollet, C. (2000). Imipenem Resistance of Enterobacter aerogenes Mediated by Outer Membrane Permeability. J. Clin. Microbiol. 38: 1048-1052 [Abstract] [Full Text]  
  • Tzelepi, E., Giakkoupi, P., Sofianou, D., Loukova, V., Kemeroglou, A., Tsakris, A. (2000). Detection of Extended-Spectrum beta -Lactamases in Clinical Isolates of Enterobacter cloacae and Enterobacter aerogenes. J. Clin. Microbiol. 38: 542-546 [Abstract] [Full Text]  
  • Marchandin, H., Jean-Pierre, H., De Champs, C., Sirot, D., Darbas, H., Perigault, P. F., Carriere, C. (2000). Production of a TEM-24 Plasmid-Mediated Extended-Spectrum beta -Lactamase by a Clinical Isolate of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 44: 213-216 [Abstract] [Full Text]  
  • Heritage, J., M'Zali, F. H., Gascoyne-Binzi, D., Hawkey, P. M. (1999). Evolution and spread of SHV extended-spectrum {beta}-lactamases in Gram-negative bacteria. J Antimicrob Chemother 44: 309-318 [Abstract] [Full Text]  
  • Bosi, C., Davin-Regli, A., Bornet, C., Mallea, M., Pages, J.-M., Bollet, C. (1999). Most Enterobacter aerogenes Strains in France Belong to a Prevalent Clone. J. Clin. Microbiol. 37: 2165-2169 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»