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Antimicrobial Agents and Chemotherapy, March 1998, p. 717-720, Vol. 42, No. 3
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Analysis of Diversity of Mutations in the mecI Gene and mecA Promoter/Operator Region of Methicillin-Resistant Staphylococcus aureus and Staphylococcus epidermidis

Nobumichi Kobayashi,* Koki Taniguchi, and Shozo Urasawa

Department of Hygiene, Sapporo Medical University School of Medicine, S-1, W-17, Chuo-ku, Sapporo, 060, Japan

Received 23 June 1997/Returned for modification 22 October 1997/Accepted 4 December 1997

Genomic diversity of mutation in the mecI gene or mecA promoter/operator region was analyzed for clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus epidermidis (MRSE). In most MRSA strains, a single base substitution was detected in either the mecI (three different positions) or the mecA promoter (two different positions), while a 28-base deletion in mecI was found in one strain. In contrast, no mutation was detected in these gene sequences of MRSE strains.

* Corresponding author. Mailing address: Department of Hygiene, Sapporo Medical University School of Medicine, S-1, W-17, Chuo-ku, Sapporo, 060, Japan. Phone: 81-11-611-2111. Fax: 81-11-612-1660. E-mail: nkobayas{at}sapmed.ac.jp.

Antimicrobial Agents and Chemotherapy, March 1998, p. 717-720, Vol. 42, No. 3
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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