This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schwank, S.
Right arrow Articles by Blaser, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schwank, S.
Right arrow Articles by Blaser, J.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, April 1998, p. 895-898, Vol. 42, No. 4
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Impact of Bacterial Biofilm Formation on In Vitro and In Vivo Activities of Antibiotics

Silvia Schwank,1 Zarko Rajacic,2 Werner Zimmerli,2 and Jürg Blaser1,*

Department of Internal Medicine, University Hospital Zurich, Zurich,1 and Department of Internal Medicine, University Hospital Basel, Basel,2 Switzerland

Received 4 August 1997/Returned for modification 16 December 1997/Accepted 24 January 1998

The impact of bacterial adherence on antibiotic activity was analyzed with two isogenic strains of Staphylococcus epidermidis that differ in the features of their in vitro biofilm formation. The eradication of bacteria adhering to glass beads by amikacin, levofloxacin, rifampin, or teicoplanin was studied in an animal model and in a pharmacokinetically matched in vitro model. The features of S. epidermidis RP62A that allowed it to grow on surfaces in multiple layers promoted phenotypic resistance to antibiotic treatment, whereas strain M7 failed to accumulate, despite initial adherence on surfaces and growth in suspension similar to those for RP62A. Biofilms of S. epidermidis M7 were better eradicated than those of strain RP62A in vitro (46 versus 31%; P < 0.05) as well as in the animal model (39 versus 9%; P < 0.01).

* Corresponding author. Mailing address: Department of Medicine, D POL 40, University Hospital Zurich, Rämistrasse 100, CH-8091 Zurich, Switzerland. Phone: 41-1-255-3618. Fax: 41-1-255-4562. E-mail: dimjbl{at}usz.unizh.ch.

Antimicrobial Agents and Chemotherapy, April 1998, p. 895-898, Vol. 42, No. 4
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Euba, G., Murillo, O., Fernandez-Sabe, N., Mascaro, J., Cabo, J., Perez, A., Tubau, F., Verdaguer, R., Gudiol, F., Ariza, J. (2009). Long-Term Follow-Up Trial of Oral Rifampin-Cotrimoxazole Combination versus Intravenous Cloxacillin in Treatment of Chronic Staphylococcal Osteomyelitis. Antimicrob. Agents Chemother. 53: 2672-2676 [Abstract] [Full Text]  
  • Baldoni, D., Haschke, M., Rajacic, Z., Zimmerli, W., Trampuz, A. (2009). Linezolid Alone or Combined with Rifampin against Methicillin-Resistant Staphylococcus aureus in Experimental Foreign-Body Infection. Antimicrob. Agents Chemother. 53: 1142-1148 [Abstract] [Full Text]  
  • Robertson, G. T., Bonventre, E. J., Doyle, T. B., Du, Q., Duncan, L., Morris, T. W., Roche, E. D., Yan, D., Lynch, A. S. (2008). In Vitro Evaluation of CBR-2092, a Novel Rifamycin-Quinolone Hybrid Antibiotic: Studies of the Mode of Action in Staphylococcus aureus. Antimicrob. Agents Chemother. 52: 2313-2323 [Abstract] [Full Text]  
  • Yeater, K. M., Chandra, J., Cheng, G., Mukherjee, P. K., Zhao, X., Rodriguez-Zas, S. L., Kwast, K. E., Ghannoum, M. A., Hoyer, L. L. (2007). Temporal analysis of Candida albicans gene expression during biofilm development. Microbiology 153: 2373-2385 [Abstract] [Full Text]  
  • Trampuz, A., Murphy, C. K., Rothstein, D. M., Widmer, A. F., Landmann, R., Zimmerli, W. (2007). Efficacy of a Novel Rifamycin Derivative, ABI-0043, against Staphylococcus aureus in an Experimental Model of Foreign-Body Infection. Antimicrob. Agents Chemother. 51: 2540-2545 [Abstract] [Full Text]  
  • Zhao, X., Daniels, K. J., Oh, S.-H., Green, C. B., Yeater, K. M., Soll, D. R., Hoyer, L. L. (2006). Candida albicans Als3p is required for wild-type biofilm formation on silicone elastomer surfaces.. Microbiology 152: 2287-2299 [Abstract] [Full Text]  
  • Al-Fattani, M. A., Douglas, L. J. (2006). Biofilm matrix of Candida albicans and Candida tropicalis: chemical composition and role in drug resistance.. J Med Microbiol 55: 999-1008 [Abstract] [Full Text]  
  • Lee, J.-Y., Ko, K. S., Peck, K. R., Oh, W. S., Song, J.-H. (2006). In vitro evaluation of the antibiotic lock technique (ALT) for the treatment of catheter-related infections caused by staphylococci. J Antimicrob Chemother 57: 1110-1115 [Abstract] [Full Text]  
  • Senn, M. M., Giachino, P., Homerova, D., Steinhuber, A., Strassner, J., Kormanec, J., Fluckiger, U., Berger-Bachi, B., Bischoff, M. (2005). Molecular Analysis and Organization of the {sigma}B Operon in Staphylococcus aureus. J. Bacteriol. 187: 8006-8019 [Abstract] [Full Text]  
  • Goerke, C., Fluckiger, U., Steinhuber, A., Bisanzio, V., Ulrich, M., Bischoff, M., Patti, J. M., Wolz, C. (2005). Role of Staphylococcus aureus Global Regulators sae and {sigma}B in Virulence Gene Expression during Device-Related Infection. Infect. Immun. 73: 3415-3421 [Abstract] [Full Text]  
  • Trebse, R., Pisot, V., Trampuz, A. (2005). Treatment of infected retained implants. J Bone Joint Surg Br 87-B: 249-256 [Abstract] [Full Text]  
  • Zimmerli, W., Trampuz, A., Ochsner, P. E. (2004). Prosthetic-Joint Infections. NEJM 351: 1645-1654 [Full Text]  
  • Kadurugamuwa, J. L., Sin, L. V., Yu, J., Francis, K. P., Kimura, R., Purchio, T., Contag, P. R. (2003). Rapid Direct Method for Monitoring Antibiotics in a Mouse Model of Bacterial Biofilm Infection. Antimicrob. Agents Chemother. 47: 3130-3137 [Abstract] [Full Text]  
  • Vaudaux, P., Francois, P., Bisognano, C., Li, D., Lew, D. P., Schrenzel, J. (2003). Comparative efficacy of daptomycin and vancomycin in the therapy of experimental foreign body infection due to Staphylococcus aureus. J Antimicrob Chemother 52: 89-95 [Abstract] [Full Text]  
  • Wolz, C., Goerke, C., Landmann, R., Zimmerli, W., Fluckiger, U. (2002). Transcription of Clumping Factor A in Attached and Unattached Staphylococcus aureus In Vitro and during Device-Related Infection. Infect. Immun. 70: 2758-2762 [Abstract] [Full Text]  
  • Vaudaux, P., Francois, P., Bisognano, C., Schrenzel, J., Lew, D. P. (2002). Comparison of Levofloxacin, Alatrofloxacin, and Vancomycin for Prophylaxis and Treatment of Experimental Foreign-Body-Associated Infection by Methicillin-Resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 46: 1503-1509 [Abstract] [Full Text]  
  • Monzon, M., Oteiza, C., Leiva, J., Amorena, B. (2001). Synergy of different antibiotic combinations in biofilms of Staphylococcus epidermidis. J Antimicrob Chemother 48: 793-801 [Abstract] [Full Text]  
  • O'GARA, J. P., HUMPHREYS, H. (2001). Staphylococcus epidermidis biofilms: importance and implications. J Med Microbiol 50: 582-587 [Abstract] [Full Text]  
  • Wilcox, M. H., Kite, P., Mills, K., Sugden, S. (2001). In situ measurement of linezolid and vancomycin concentrations in intravascular catheter-associated biofilm. J Antimicrob Chemother 47: 171-175 [Abstract] [Full Text]  
  • Rachid, S., Ohlsen, K., Witte, W., Hacker, J., Ziebuhr, W. (2000). Effect of Subinhibitory Antibiotic Concentrations on Polysaccharide Intercellular Adhesin Expression in Biofilm-Forming Staphylococcus epidermidis. Antimicrob. Agents Chemother. 44: 3357-3363 [Abstract] [Full Text]  
  • Veyries, M.-L., Faurisson, F., Joly-Guillou, M.-L., Rouveix, B. (2000). Control of Staphylococcal Adhesion to Polymethylmethacrylate and Enhancement of Susceptibility to Antibiotics by Poloxamer 407. Antimicrob. Agents Chemother. 44: 1093-1096 [Abstract] [Full Text]  
  • Truninger, K, Jost, C H A., Seifert, B, Vogt, P R, Follath, F, Schaffner, A, Jenni, R (1999). Long term follow up of prosthetic valve endocarditis: what characteristics identify patients who were treated successfully with antibiotics alone?. Heart 82: 714-720 [Abstract] [Full Text]  
  • Krzywda, E. A., Andris, D. A., Edmiston, C. E. Jr (1999). Catheter Infections: Diagnosis, Etiology, Treatment, and Prevention. Nutr Clin Pract 14: 178-190 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»