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Antimicrobial Agents and Chemotherapy, May 1998, p. 1110-1114, Vol. 42, No. 5
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Chromosomally Encoded AmpC-Type beta -Lactamase in a Clinical Isolate of Proteus mirabilis

L. Bret,1,* C. Chanal-Claris,1 D. Sirot,1 E. B. Chaibi,2 R. Labia,2 and J. Sirot1

Laboratoire de Bactériologie, Faculté de Médecine, 63001 Clermont-Ferrand Cedex,1 and UMR 175, CNRS-MNHN, 29000 Quimper,2 France

Received 30 July 1997/Returned for modification 30 November 1997/Accepted 8 March 1998

A clinical strain of Proteus mirabilis (CF09) isolated from urine specimens of a patient displayed resistance to amoxicillin (MIC >4,096 µg/ml), ticarcillin (4,096 µg/ml), cefoxitin (64 µg/ml), cefotaxime (256 µg/ml), and ceftazidime (128 µg/ml) and required an elevated MIC of aztreonam (4 µg/ml). Clavulanic acid did not act synergistically with cephalosporins. Two beta -lactamases with apparent pIs of 5.6 and 9.0 were identified by isoelectric focusing on a gel. Substrate and inhibition profiles were characteristic of an AmpC-type beta -lactamase with a pI of 9.0. Amplification by PCR with primers for ampC genes (Escherichia coli, Enterobacter cloacae, and Citrobacter freundii) of a 756-bp DNA fragment from strain CF09 was obtained only with C. freundii-specific primers. Hybridization results showed that the ampC gene is only chromosomally located while the TEM gene is plasmid located. After cloning of the gene, analysis of the complete nucleotide sequence (1,146 bp) showed that this ampC gene is close to blaCMY-2, from which it differs by three point mutations leading to amino acid substitutions Glu right-arrow Gly at position 22, Trp right-arrow Arg at position 201, and Ser right-arrow Asn at position 343. AmpC beta -lactamases derived from that of C. freundii (LAT-1, LAT-2, BIL-1, and CMY-2) have been found in Klebsiella pneumoniae, E. coli, and Enterobacter aerogenes and have been reported to be plasmid borne. This is the first example of a chromosomally encoded AmpC-type beta -lactamase observed in P. mirabilis. We suggest that it be designated CMY-3.

* Corresponding author. Mailing address: Laboratoire de Bactériologie, Faculté de Médecine, 28 Place Henri-Dunant, 63001 Clermont-Ferrand Cedex, France. Phone: 33 4 73 60 80 18. Fax: 33 4 73 27 74 94. E-mail: Danielle.SIROT{at}U-Clermont1.fr.

Antimicrobial Agents and Chemotherapy, May 1998, p. 1110-1114, Vol. 42, No. 5
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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