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Antimicrobial Agents and Chemotherapy, May 1998, p. 1259-1262, Vol. 42, No. 5
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Sequence of the Gene Encoding a Plasmid-Mediated Cefotaxime-Hydrolyzing Class A beta -Lactamase (CTX-M-4): Involvement of Serine 237 in Cephalosporin Hydrolysis

Maria Gazouli,1 Eva Tzelepi,1 Sergei V. Sidorenko,2 and Leonidas S. Tzouvelekis1,*

Department of Bacteriology, Hellenic Pasteur Institute, Athens 11521, Greece,1 and National Research Center for Antibiotics, Moscow, Russia2

Received 15 September 1997/Returned for modification 5 January 1998/Accepted 8 February 1998

The sequence of the gene encoding a novel cefotaxime-hydrolyzing beta -lactamase (CTX-M-4) was determined. It was located in a plasmid harbored by a Salmonella typhimurium strain. CTX-M-4 was similar to the plasmidic cefotaxime-hydrolyzing beta -lactamases CTX-M-2 and Toho-1 and related to the chromosomal beta -lactamase of Klebsiella oxytoca. A Ser-237right-arrowAla substitution, introduced by site-directed mutagenesis, caused minor alterations in the interaction of CTX-M-4 with beta -lactams, reducing slightly the relative hydrolytic activity against cefotaxime and the susceptibility to inhibition by clavulanate.

* Corresponding author. Mailing address: Department of Bacteriology, Hellenic Pasteur Institute, Vas. Sofias Ave. 127, Athens 11521, Greece. Phone: 30 (1) 6462281. Fax: 30 (1) 6423498. E-mail: Lstbact{at}hotmail.com.

Antimicrobial Agents and Chemotherapy, May 1998, p. 1259-1262, Vol. 42, No. 5
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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