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Antimicrobial Agents and Chemotherapy, June 1998, p. 1459-1462, Vol. 42, No. 6
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Ceftazidime and Aztreonam Resistance in Providencia stuartii: Characterization of a Natural TEM-Derived ExtendedSpectrum beta -Lactamase, TEM-60

Nicola Franceschini,1 Mariagrazia Perilli,1 Bernardetta Segatore,1 Domenico Setacci,1 Gianfranco Amicosante,1,* Annarita Mazzariol,2 and Giuseppe Cornaglia2

Dipartimento di Scienze e Tecnologie Biomediche, Università degli Studi dell'Aquila, Loc. Coppito I-67010, L'Aquila,1 and Istituto di Microbiologia, Università degli Studi di Verona, I-37134, Verona,2 Italy

Received 11 August 1997/Returned for modification 1 December 1997/Accepted 30 March 1998

A plasmid-encoded beta -lactamase produced from a clinical strain of Providencia stuartii has been purified and characterized. The gene coding for the beta -lactamase was cloned and sequenced. It appears to be a new natural TEM-derived enzyme, named TEM-60. Point mutations (Q39K, L51P, E104K, and R164S) are present with respect to the TEM-1 enzyme; the mutation L51P has never been previously reported, with the exception of the chromosomally encoded extended-spectrum beta -lactamase PER-1. Kinetic parameters relative to penicillins, cephalosporins, and monobactams other than mechanism-based inactivators were related to the in vitro susceptibility phenotype.

* Corresponding author. Mailing address: Dipartimento di Scienze e Tecnologie Biomediche Univ. degli Studi dell'Aquila Loc. Coppito 1-67010, L'Aquila, Italy. Phone: 39 862 433455. Fax: 39 862 433433. E-mail: Amicosante{at}aquila.infn.it.

Antimicrobial Agents and Chemotherapy, June 1998, p. 1459-1462, Vol. 42, No. 6
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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