This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ardanuy, C.
Right arrow Articles by Martínez-Martínez, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ardanuy, C.
Right arrow Articles by Martínez-Martínez, L.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, July 1998, p. 1636-1640, Vol. 42, No. 7
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Outer Membrane Profiles of Clonally Related Klebsiella pneumoniae Isolates from Clinical Samples and Activities of Cephalosporins and Carbapenems

Carmen Ardanuy,1,* Josefina Liñares,1 María Angeles Domínguez,1 Santiago Hernández-Allés,2 Vicente J. Benedí,2 and Luis Martínez-Martínez3

Servicio de Microbiología, Hospital de Bellvitge, Universidad de Barcelona, Barcelona,1 Departamento de Biología Ambiental, Universidad de las Islas Baleares and IMEDEA (CSIC-UIB), Palma de Mallorca,2 and Departamento de Microbiología, Facultad de Medicina, Universidad de Sevilla, Seville,3 Spain

Received 19 November 1997/Returned for modification 11 February 1998/Accepted 16 April 1998

Fifteen isolates of Klebsiella pneumoniae producing extended-spectrum beta -lactamases (ESBLs) isolated during a nosocomial outbreak were studied. The strains belonged to the same clonal type, as shown by pulsed-field gel electrophoretic analysis of chromosomal DNA. All the isolates were resistant to extended-spectrum cephalosporins, aztreonam, gentamicin, and fluoroquinolones and were susceptible to carbapenems, tobramycin, netilmicin, and amikacin. None of the isolates expressed the OmpK36 porin. Eight isolates, for which the MICs of cefoxitin were >= 64 µg/ml, showed a diminished level or no expression of a 35-kDa porin. The MICs of meropenem, cefotaxime, and cefpirome were three to eight times higher for porin-deficient isolates than for isolates expressing the 35-kDa porin, but the MICs of imipenem increased two times for porin-deficient isolates compared to those for isolates expressing the porin. This MIC increase reverted to a level similar to that for the parental strain when porin-deficient isolates were transformed with the gene coding for the K. pneumoniae porin OmpK36. It is concluded that the high level of resistance to cefoxitin and the increase in the MICs of meropenem, cefotaxime, and cefpirome for the ESBL-producing K. pneumoniae isolates studied are associated with porin deficiency.

* Corresponding author. Mailing address: Servicio de Microbiología, Hospital de Bellvitge, Feixa Llarga s/n. 08907, L'Hospitalet, Barcelona, Spain. Phone: 34-3-3357011, ext. 2097. Fax: 34-93-2607547. E-mail: c.ardanuy{at}csub.scs.es.

Antimicrobial Agents and Chemotherapy, July 1998, p. 1636-1640, Vol. 42, No. 7
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Landman, D., Bratu, S., Quale, J. (2009). Contribution of OmpK36 to carbapenem susceptibility in KPC-producing Klebsiella pneumoniae. J Med Microbiol 58: 1303-1308 [Abstract] [Full Text]  
  • Tran, Q.-T., Dupont, M., Lavigne, J.-P., Chevalier, J., Pages, J.-M., Sotto, A., Davin-Regli, A. (2009). Occurrence of Efflux Mechanism and Cephalosporinase Variant in a Population of Enterobacter aerogenes and Klebsiella pneumoniae Isolates Producing Extended-Spectrum {beta}-Lactamases. Antimicrob. Agents Chemother. 53: 1652-1656 [Abstract] [Full Text]  
  • Kaczmarek, F. M., Dib-Hajj, F., Shang, W., Gootz, T. D. (2006). High-Level Carbapenem Resistance in a Klebsiella pneumoniae Clinical Isolate Is Due to the Combination of blaACT-1 {beta}-Lactamase Production, Porin OmpK35/36 Insertional Inactivation, and Down-Regulation of the Phosphate Transport Porin PhoE.. Antimicrob. Agents Chemother. 50: 3396-3406 [Abstract] [Full Text]  
  • Virginia Villegas, M., Lolans, K., Correa, A., Jose Suarez, C., Lopez, J. A., Vallejo, M., Quinn, J. P., the Colombian Nosocomial Resistance Study Group, (2006). First Detection of the Plasmid-Mediated Class A Carbapenemase KPC-2 in Clinical Isolates of Klebsiella pneumoniae from South America.. Antimicrob. Agents Chemother. 50: 2880-2882 [Abstract] [Full Text]  
  • Padilla, E., Alonso, D., Domenech-Sanchez, A., Gomez, C., Perez, J. L., Alberti, S., Borrell, N. (2006). Effect of Porins and Plasmid-Mediated AmpC {beta}-Lactamases on the Efficacy of {beta}-Lactams in Rat Pneumonia Caused by Klebsiella pneumoniae.. Antimicrob. Agents Chemother. 50: 2258-2260 [Abstract] [Full Text]  
  • Paterson, D. L., Bonomo, R. A. (2005). Extended-Spectrum {beta}-Lactamases: a Clinical Update. Clin. Microbiol. Rev. 18: 657-686 [Abstract] [Full Text]  
  • Literacka, E., Empel, J., Baraniak, A., Sadowy, E., Hryniewicz, W., Gniadkowski, M. (2004). Four Variants of the Citrobacter freundii AmpC-Type Cephalosporinases, Including Novel Enzymes CMY-14 and CMY-15, in a Proteus mirabilis Clone Widespread in Poland. Antimicrob. Agents Chemother. 48: 4136-4143 [Abstract] [Full Text]  
  • Poirel, L., Heritier, C., Tolun, V., Nordmann, P. (2004). Emergence of Oxacillinase-Mediated Resistance to Imipenem in Klebsiella pneumoniae. Antimicrob. Agents Chemother. 48: 15-22 [Abstract] [Full Text]  
  • Yigit, H., Queenan, A. M., Rasheed, J. K., Biddle, J. W., Domenech-Sanchez, A., Alberti, S., Bush, K., Tenover, F. C. (2003). Carbapenem-Resistant Strain of Klebsiella oxytoca Harboring Carbapenem-Hydrolyzing {beta}-Lactamase KPC-2. Antimicrob. Agents Chemother. 47: 3881-3889 [Abstract] [Full Text]  
  • Domenech-Sanchez, A., Martinez-Martinez, L., Hernandez-Alles, S., del Carmen Conejo, M., Pascual, A., Tomas, J. M., Alberti, S., Benedi, V. J. (2003). Role of Klebsiella pneumoniae OmpK35 Porin in Antimicrobial Resistance. Antimicrob. Agents Chemother. 47: 3332-3335 [Abstract] [Full Text]  
  • Melano, R., Corso, A., Petroni, A., Centron, D., Orman, B., Pereyra, A., Moreno, N., Galas, M. (2003). Multiple antibiotic-resistance mechanisms including a novel combination of extended-spectrum {beta}-lactamases in a Klebsiella pneumoniae clinical strain isolated in Argentina. J Antimicrob Chemother 52: 36-42 [Abstract] [Full Text]  
  • Gayet, S., Chollet, R., Molle, G., Pages, J.-M., Chevalier, J. (2003). Modification of Outer Membrane Protein Profile and Evidence Suggesting an Active Drug Pump in Enterobacter aerogenes Clinical Strains. Antimicrob. Agents Chemother. 47: 1555-1559 [Abstract] [Full Text]  
  • Martinez-Martinez, L., Pascual, A., Conejo, M. d. C., Garcia, I., Joyanes, P., Domenech-Sanchez, A., Benedi, V. J. (2002). Energy-Dependent Accumulation of Norfloxacin and Porin Expression in Clinical Isolates of Klebsiella pneumoniae and Relationship to Extended-Spectrum {beta}-Lactamase Production. Antimicrob. Agents Chemother. 46: 3926-3932 [Abstract] [Full Text]  
  • Crowley, B., Benedi, V. J., Domenech-Sanchez, A. (2002). Expression of SHV-2 {beta}-Lactamase and of Reduced Amounts of OmpK36 Porin in Klebsiella pneumoniae Results in Increased Resistance to Cephalosporins and Carbapenems. Antimicrob. Agents Chemother. 46: 3679-3682 [Abstract] [Full Text]  
  • Sahloff, E. G., Martin, S. J. (2002). Extended-Spectrum {beta}-Lactamase Resistance in the ICU. Journal of Pharmacy Practice 15: 96-105 [Abstract]  
  • Steward, C. D., Rasheed, J. K., Hubert, S. K., Biddle, J. W., Raney, P. M., Anderson, G. J., Williams, P. P., Brittain, K. L., Oliver, A., McGowan, J. E. Jr., Tenover, F. C. (2001). Characterization of Clinical Isolates of Klebsiella pneumoniae from 19 Laboratories Using the National Committee for Clinical Laboratory Standards Extended-Spectrum {beta}-Lactamase Detection Methods. J. Clin. Microbiol. 39: 2864-2872 [Abstract] [Full Text]  
  • Wu, T.-L., Siu, L. K., Su, L.-H., Lauderdale, T. L., Lin, F. M., Leu, H.-S., Lin, T.-Y., Ho, M. (2001). Outer membrane protein change combined with co-existing TEM-1 and SHV-1 {beta}-lactamases lead to false identification of ESBL-producing Klebsiella pneumoniae. J Antimicrob Chemother 47: 755-761 [Abstract] [Full Text]  
  • Yigit, H., Queenan, A. M., Anderson, G. J., Domenech-Sanchez, A., Biddle, J. W., Steward, C. D., Alberti, S., Bush, K., Tenover, F. C. (2001). Novel Carbapenem-Hydrolyzing {beta}-Lactamase, KPC-1, from a Carbapenem-Resistant Strain of Klebsiella pneumoniae. Antimicrob. Agents Chemother. 45: 1151-1161 [Abstract] [Full Text]  
  • Rebuck, J. A., Olsen, K. M., Fey, P. D., Bergman, K. L., Rupp, M. E. (2000). In vitro activities of parenteral {beta}-lactam antimicrobials against TEM-10-, TEM-26- and SHV-5-derived extended-spectrum {beta}-lactamases expressed in an isogenic Escherichia coli host. J Antimicrob Chemother 46: 461-464 [Abstract] [Full Text]  
  • Coudron, P. E., Moland, E. S., Thomson, K. S. (2000). Occurrence and Detection of AmpC Beta-Lactamases among Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis Isolates at a Veterans Medical Center. J. Clin. Microbiol. 38: 1791-1796 [Abstract] [Full Text]  
  • Doménech-Sánchez, A., Hernández-Allés, S., Martínez-Martínez, L., Benedí, V. J., Albertí, S. (1999). Identification and Characterization of a New Porin Gene of Klebsiella pneumoniae: Its Role in beta -Lactam Antibiotic Resistance. J. Bacteriol. 181: 2726-2732 [Abstract] [Full Text]  
  • Hernández-Allés, S., Benedí, V. J., Martínez-Martínez, L., Pascual, A., Aguilar, A., Tomás, J. M., Albertí, S. (1999). Development of Resistance during Antimicrobial Therapy Caused by Insertion Sequence Interruption of Porin Genes. Antimicrob. Agents Chemother. 43: 937-939 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»