This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yang, Y.
Right arrow Articles by Bush, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yang, Y.
Right arrow Articles by Bush, K.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, July 1998, p. 1671-1676, Vol. 42, No. 7
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Ceftazidime-Resistant Klebsiella pneumoniae and Escherichia coli Isolates Producing TEM-10 and TEM-43 beta -Lactamases from St. Louis, Missouri

Youjun Yang,1,* Niraja Bhachech,1 Patricia A. Bradford,1 Bradley D. Jett,2 Daniel F. Sahm,2,dagger and Karen Bush1,Dagger

Wyeth-Ayerst Research, Pearl River, New York,1 and Barnes-Jewish Hospital, Washington University School of Medicine, St. Louis, Missouri2

Received 3 November 1997/Returned for modification 24 February 1998/Accepted 9 April 1998

Ceftazidime-resistant Escherichia coli and Klebsiella pneumoniae (49 and 102 isolates, respectively) were collected from Barnes-Jewish Hospital, St. Louis, Mo., from 1992 to 1996. They were uniformly resistant to ceftazidime, generally resistant to aztreonam, and variably susceptible to cefotaxime. Four representative E. coli strains and 15 Klebsiella strains were examined. From one to four beta -lactamases were produced per strain, with three possible enzymes related to ceftazidime resistance: enzymes with pI values of 5.6, 6.1, or 7.6. By pulsed-field gel electrophoresis there were at least 13 different Klebsiella strain types and 3 different E. coli strain types, indicating that the outbreak was not clonal. After cloning and sequencing of the beta -lactamase-encoding genes, the enzyme with a pI of 5.6 was identified as TEM-10. The enzyme with a pI of 6.1 was a novel TEM variant (TEM-43) with Lys at 104, His at 164, and Thr at 182. TEM-43 showed broad-spectrum hydrolytic activity against all penicillins, with the highest hydrolysis rate for ceftazidime compared to those for the other expanded-spectrum cephalosporins. Aztreonam was also a good substrate for TEM-43, with hydrolytic activity similar to that of ceftazidime and affinity higher than that of ceftazidime. The TEM-43 beta -lactamase was well inhibited by clavulanic acid and tazobactam at concentrations of <10 nM. Sulbactam was less effective than the other inhibitors. The Thr182 mutation previously reported in an inhibitor-resistant beta -lactamase did not cause the TEM-43 enzyme to become resistant to any of the inhibitors.

* Corresponding author. Mailing address: 205/227, Infectious Disease Research, Wyeth-Ayerst, 401, North Middletown Rd., Pearl River, NY 10965. Phone: (914) 732-4466. Fax: (914) 732-2480. E-mail: Yangy{at}war.wyeth.com.

dagger Present address: MRL Pharmaceutical Services Inc., Reston, Va.

Dagger Present address: R. W. Johnson Pharmaceutical Research Institute, Raritan, N.J.

Antimicrobial Agents and Chemotherapy, July 1998, p. 1671-1676, Vol. 42, No. 7
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Jones, C. H., Ruzin, A., Tuckman, M., Visalli, M. A., Petersen, P. J., Bradford, P. A. (2009). Pyrosequencing Using the Single-Nucleotide Polymorphism Protocol for Rapid Determination of TEM- and SHV-Type Extended-Spectrum {beta}-Lactamases in Clinical Isolates and Identification of the Novel {beta}-Lactamase Genes blaSHV-48, blaSHV-105, and blaTEM-155. Antimicrob. Agents Chemother. 53: 977-986 [Abstract] [Full Text]  
  • Petersen, P. J., Jones, C. H., Venkatesan, A. M., Mansour, T. S., Projan, S. J., Bradford, P. A. (2009). Establishment of In Vitro Susceptibility Testing Methodologies and Comparative Activities of Piperacillin in Combination with the Penem {beta}-Lactamase Inhibitor BLI-489. Antimicrob. Agents Chemother. 53: 370-384 [Abstract] [Full Text]  
  • Lachmayr, K. L., Kerkhof, L. J., DiRienzo, A. G., Cavanaugh, C. M., Ford, T. E. (2009). Quantifying Nonspecific TEM {beta}-Lactamase (blaTEM) Genes in a Wastewater Stream. Appl. Environ. Microbiol. 75: 203-211 [Abstract] [Full Text]  
  • Paterson, D. L., Bonomo, R. A. (2005). Extended-Spectrum {beta}-Lactamases: a Clinical Update. Clin. Microbiol. Rev. 18: 657-686 [Abstract] [Full Text]  
  • Chia, J.-H., Chu, C., Su, L.-H., Chiu, C.-H., Kuo, A.-J., Sun, C.-F., Wu, T.-L. (2005). Development of a Multiplex PCR and SHV Melting-Curve Mutation Detection System for Detection of Some SHV and CTX-M {beta}-Lactamases of Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae in Taiwan. J. Clin. Microbiol. 43: 4486-4491 [Abstract] [Full Text]  
  • Hammond, D. S., Schooneveldt, J. M., Nimmo, G. R., Huygens, F., Giffard, P. M. (2005). blaSHV Genes in Klebsiella pneumoniae: Different Allele Distributions Are Associated with Different Promoters within Individual Isolates. Antimicrob. Agents Chemother. 49: 256-263 [Abstract] [Full Text]  
  • Weiss, W. J., Petersen, P. J., Murphy, T. M., Tardio, L., Yang, Y., Bradford, P. A., Venkatesan, A. M., Abe, T., Isoda, T., Mihira, A., Ushirogochi, H., Takasake, T., Projan, S., O'Connell, J., Mansour, T. S. (2004). In Vitro and In Vivo Activities of Novel 6-Methylidene Penems as {beta}-Lactamase Inhibitors. Antimicrob. Agents Chemother. 48: 4589-4596 [Abstract] [Full Text]  
  • Queenan, A. M., Foleno, B., Gownley, C., Wira, E., Bush, K. (2004). Effects of Inoculum and {beta}-Lactamase Activity in AmpC- and Extended-Spectrum {beta}-Lactamase (ESBL)-Producing Escherichia coli and Klebsiella pneumoniae Clinical Isolates Tested by Using NCCLS ESBL Methodology. J. Clin. Microbiol. 42: 269-275 [Abstract] [Full Text]  
  • Sanguinetti, M., Posteraro, B., Spanu, T., Ciccaglione, D., Romano, L., Fiori, B., Nicoletti, G., Zanetti, S., Fadda, G. (2003). Characterization of Clinical Isolates of Enterobacteriaceae from Italy by the BD Phoenix Extended-Spectrum {beta}-Lactamase Detection Method. J. Clin. Microbiol. 41: 1463-1468 [Abstract] [Full Text]  
  • Perilli, M., Dell'Amico, E., Segatore, B., de Massis, M. R., Bianchi, C., Luzzaro, F., Rossolini, G. M., Toniolo, A., Nicoletti, G., Amicosante, G. (2002). Molecular Characterization of Extended-Spectrum {beta}-Lactamases Produced by Nosocomial Isolates of Enterobacteriaceae from an Italian Nationwide Survey. J. Clin. Microbiol. 40: 611-614 [Abstract] [Full Text]  
  • Bradford, P. A. (2001). Extended-Spectrum {beta}-Lactamases in the 21st Century: Characterization, Epidemiology, and Detection of This Important Resistance Threat. Clin. Microbiol. Rev. 14: 933-951 [Abstract] [Full Text]  
  • Essack, S. Y., Hall, L. M. C., Pillay, D. G., McFadyen, M. L., Livermore, D. M. (2001). Complexity and Diversity of Klebsiella pneumoniae Strains with Extended-Spectrum {beta}-Lactamases Isolated in 1994 and 1996 at a Teaching Hospital in Durban, South Africa. Antimicrob. Agents Chemother. 45: 88-95 [Abstract] [Full Text]  
  • Sideraki, V., Huang, W., Palzkill, T., Gilbert, H. F. (2000). A secondary drug resistance mutation of TEM-1 beta -lactamase that suppresses misfolding and aggregation. Proc. Natl. Acad. Sci. USA 10.1073/pnas.011454198v1 [Abstract] [Full Text]  
  • Perilli, M., Segatore, B., Rosaria De Massis, M., Riccio, M. L., Bianchi, C., Zollo, A., Rossolini, G. M., Amicosante, G. (2000). TEM-72, a New Extended-Spectrum beta -Lactamase Detected in Proteus mirabilis and Morganella morganii in Italy. Antimicrob. Agents Chemother. 44: 2537-2539 [Abstract] [Full Text]  
  • Bradford, P. A., Yang, Y., Sahm, D., Grope, I., Gardovska, D., Storch, G. (1998). CTX-M-5, a Novel Cefotaxime-Hydrolyzing beta -Lactamase from an Outbreak of Salmonella typhimurium in Latvia. Antimicrob. Agents Chemother. 42: 1980-1984 [Abstract] [Full Text]  
  • Sideraki, V., Huang, W., Palzkill, T., Gilbert, H. F. (2001). A secondary drug resistance mutation of TEM-1 beta -lactamase that suppresses misfolding and aggregation. Proc. Natl. Acad. Sci. USA 98: 283-288 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»