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Antimicrobial Agents and Chemotherapy, July 1998, p. 1794-1798, Vol. 42, No. 7
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Characterization of a Staphylococcal Plasmid Related to pUB110 and Carrying Two Novel Genes, vatC and vgbB, Encoding Resistance to Streptogramins A and B and Similar Antibiotics

Jeanine Allignet, Nadia Liassine,dagger and Névine El Solh*

National Reference Center for Staphylococci, Unité des Staphylocoques, Institut Pasteur, 75724 Paris Cedex 15, France

Received 19 November 1997/Returned for modification 20 February 1998/Accepted 14 April 1998

We isolated and sequenced a plasmid, named pIP1714 (4,978 bp), which specifies resistance to streptogramins A and B and the mixture of these compounds. pIP1714 was isolated from a Staphylococcus cohnii subsp. cohnii strain found in the environment of a hospital where pristinamycin was extensively used. Resistance to both compounds and related antibiotics is encoded by two novel, probably cotranscribed genes, (i) vatC, encoding a 212-amino-acid (aa) acetyltransferase that inactivates streptogramin A and that exhibits 58.2 to 69.8% aa identity with the Vat, VatB, and SatA proteins, and (ii) vgbB, encoding a 295-aa lactonase that inactivates streptogramin B and that shows 67% aa identity with the Vgb lactonase. pIP1714 includes a 2,985-bp fragment also found in two rolling-circle replication and mobilizable plasmids, pUB110 and pBC16, from gram-positive bacteria. In all three plasmids, the common fragment was delimited by two direct repeats of four nucleotides (GGGC) and included (i) putative genes closely related to repB, which encodes a replication protein, and to pre(mob), which encodes a protein required for conjugative mobilization and site-specific recombination, and (ii) sequences very similar to the double- and single-strand origins (dso, ssoU) and the recombination site, RSA. The antibiotic resistance genes repB and pre(mob) carried by each of these plasmids were found in the same transcriptional orientation.

* Corresponding author. Mailing address: National Reference Center for Staphylococci, Unité des Staphylocoques, Institut Pasteur, 75724 Paris Cedex 15, France. Phone: (33) 01 45 68 83 63. Fax: (33) 01 40 61 31 63. E-mail: nelsolh{at}pasteur.fr.

dagger Present address: Laboratoire Central de Bactériologie, Hôpital Cantonal Universitaire, Geneva, Switzerland.

Antimicrobial Agents and Chemotherapy, July 1998, p. 1794-1798, Vol. 42, No. 7
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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