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Antimicrobial Agents and Chemotherapy, August 1998, p. 2119-2121, Vol. 42, No. 8
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Active Efflux of Norfloxacin by Bacteroides fragilis

Shin Miyamae,1,2 Hiroshi Nikaido,3,* Yoshinobu Tanaka,2 and Fuminobu Yoshimura1

Department of Microbiology1 and the First Department of Prosthodontics,2 School of Dentistry, Aichi-Gakuin University, Nagoya 464-8650, Japan, and Department of Molecular and Cell Biology, University of California, Berkeley, California 947203

Received 29 January 1998/Returned for modification 13 May 1998/Accepted 1 June 1998

Norfloxacin was actively pumped out by Bacteroides fragilis, which is intrinsically resistant to most fluoroquinolones. Reserpine moderately inhibited the efflux. A one-step spontaneous mutant had increased resistance to norfloxacin, ethidium bromide, and puromycin, a result suggesting that the efflux is catalyzed by a multidrug pump with specificity similar to that of NorA/Bmr.

* Corresponding author. Mailing address: Department of Molecular and Cell Biology, 229 Stanley Hall, University of California, Berkeley, CA 94720-3206. Phone: (510) 642-2027. Fax: (510) 643-9290. E-mail: nhiroshi{at}uclink4.berkeley.edu.

Antimicrobial Agents and Chemotherapy, August 1998, p. 2119-2121, Vol. 42, No. 8
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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