This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Llanes, C.
Right arrow Articles by Plesiat, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Llanes, C.
Right arrow Articles by Plesiat, P.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, October 1999, p. 2430-2436, Vol. 43, No. 10
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Propagation of TEM- and PSE-Type beta -Lactamases among Amoxicillin-Resistant Salmonella spp. Isolated in France

Catherine Llanes,1,* Veronique Kirchgesner,2 and Patrick Plesiat1

Laboratoire de Bactériologie, Faculté de Médecine, Université de Franche-Comté, 25030 Besançon,1 and Centre Hospitalier Général Châlon sur Saône 71000,2 France

Received 5 February 1999/Returned for modification 31 May 1999/Accepted 10 August 1999

A survey conducted between 1987 and 1994 at the University Hospital of Besançon, France, demonstrated a dramatic increase (from 0 to 42.5%) in the prevalence of amoxicillin resistance among Salmonella spp. Of the 96 resistant isolates collected during this period (including 77 Typhimurium), 54 were found to produce TEM-1 beta -lactamase, 40 produced PSE-1 (equivalent to CARB-2), one produced PSE-1 plus TEM-2, and one produced OXA-1 in isoelectric focusing and DNA hybridization experiments. Plasmids coding for these beta -lactamases were further characterized by (i) profile analysis, (ii) restriction fragmentation pattern analysis, (iii) hybridization with an spvCD-orfE virulence probe, and (iv) replicon typing. In addition, isolates of S. typhimurium were genotypically compared by pulsed-field gel electrophoresis of XbaI-macrorestricted chromosomal DNA. Altogether, these methods showed that 40 of the 41 PSE-1 producers were actually the progeny of a single epidemic S. typhimurium strain lysotype DT104. Isolates of that strain were found to harbor RepFIC virulence plasmids with somewhat different restriction profiles, but which all carried the blaPSE-1 gene. Of these virulence/resistance plasmids, 15 were transmissible to Escherichia coli. TEM-1-producing S. typhimurium displayed much greater genotypic and plasmidic diversities, suggesting the acquisition of the blaTEM-1 gene from multiple bacterial sources by individual strains. In agreement with this, 32 of the 35 S. typhimurium plasmids encoding TEM-1 were found to be conjugative. These data show that development of amoxicillin resistance among Salmonella, especially in serovar Typhimurium, results from both gene transfers and strain dissemination.

* Corresponding author. Mailing address: Laboratoire de Bactériologie, Hôpital Jean Minjoz, 25030 Besançon, France. Phone: (33)-81-66-82-86. Fax: (33)-81-66-89-14. E-mail: patrick.plesiat{at}ufc-chu.univ-fcomte.fr.

Antimicrobial Agents and Chemotherapy, October 1999, p. 2430-2436, Vol. 43, No. 10
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Foley, S. L., Lynne, A. M. (2008). Food animal-associated Salmonella challenges: Pathogenicity and antimicrobial resistance. J ANIM SCI 86: E173-E187 [Abstract] [Full Text]  
  • Villa, L., Carattoli, A. (2005). Integrons and Transposons on the Salmonella enterica Serovar Typhimurium Virulence Plasmid. Antimicrob. Agents Chemother. 49: 1194-1197 [Abstract] [Full Text]  
  • Chiu, C.-H., Su, L.-H., Chu, C. (2004). Salmonella enterica Serotype Choleraesuis: Epidemiology, Pathogenesis, Clinical Disease, and Treatment. Clin. Microbiol. Rev. 17: 311-322 [Abstract] [Full Text]  
  • Orman, B. E., Pineiro, S. A., Arduino, S., Galas, M., Melano, R., Caffer, M. I., Sordelli, D. O., Centron, D. (2002). Evolution of Multiresistance in Nontyphoid Salmonella Serovars from 1984 to 1998 in Argentina. Antimicrob. Agents Chemother. 46: 3963-3970 [Abstract] [Full Text]  
  • Guerra, B., Soto, S., Helmuth, R., Mendoza, M. C. (2002). Characterization of a Self-Transferable Plasmid from Salmonella enterica Serotype Typhimurium Clinical Isolates Carrying Two Integron-Borne Gene Cassettes Together with Virulence and Drug Resistance Genes. Antimicrob. Agents Chemother. 46: 2977-2981 [Abstract] [Full Text]  
  • Chiu, C.-H., Chu, C., Su, L.-H., Wu, W.-Y., Wu, T.-L. (2002). Characterization of a Laboratory-Derived, High-Level Ampicillin-Resistant Salmonella enterica Serovar Typhimurium Strain That Caused Meningitis in an Infant. Antimicrob. Agents Chemother. 46: 1604-1606 [Abstract] [Full Text]  
  • Chu, C., Chiu, C.-H., Wu, W.-Y., Chu, C.-H., Liu, T.-P., Ou, J. T. (2001). Large Drug Resistance Virulence Plasmids of Clinical Isolates of Salmonella enterica Serovar Choleraesuis. Antimicrob. Agents Chemother. 45: 2299-2303 [Abstract] [Full Text]  
  • Breuil, J., Brisabois, A., Casin, I., Armand-Lefevre, L., Fremy, S., Collatz, E. (2000). Antibiotic resistance in salmonellae isolated from humans and animals in France: comparative data from 1994 and 1997. J Antimicrob Chemother 46: 965-971 [Abstract] [Full Text]  
  • Guerra, B., Soto, S., Cal, S., Mendoza, M. C. (2000). Antimicrobial Resistance and Spread of Class 1 Integrons among Salmonella Serotypes. Antimicrob. Agents Chemother. 44: 2166-2169 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»