This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Scarpellini, P.
Right arrow Articles by Lazzarin, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Scarpellini, P.
Right arrow Articles by Lazzarin, A.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, October 1999, p. 2550-2554, Vol. 43, No. 10
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Detection of Rifampin Resistance in Mycobacterium tuberculosis by Double Gradient-Denaturing Gradient Gel Electrophoresis

Paolo Scarpellini,1,* Sergio Braglia,1 Paola Carrera,2 Maura Cedri,1 Paola Cichero,3 Alessia Colombo,1 Rosella Crucianelli,2 Andrea Gori,4 Maurizio Ferrari,2 and Adriano Lazzarin1

Infectious Diseases Division,1 Clinical Molecular Biology Laboratory,2 and Microbiology Laboratory,3 San Raffaele Scientific Institute, and Clinic of Infectious Diseases, University of Milan,4 Milan, Italy

Received 26 October 1998/Returned for modification 14 December 1998/Accepted 13 August 1999

We applied double gradient-denaturing gradient gel electrophoresis (DG-DGGE) for the rapid detection of rifampin (RMP) resistance from rpoB PCR products of Mycobacterium tuberculosis isolates and clinical samples. The results of this method were fully concordant with those of DNA sequencing and susceptibility testing analyses. DG-DGGE is a valid alternative to the other methods of detecting mutations for predicting RMP resistance.

* Corresponding author. Mailing address: Infectious Diseases Division, San Raffaele Scientific Institute, Via Stamira D'Ancona 20, 20127 Milan, Italy. Phone: 39-02-2643-7985. Fax: 39-02-2643-7989. E-mail: scarpellini.paolo{at}hsr.it.

Antimicrobial Agents and Chemotherapy, October 1999, p. 2550-2554, Vol. 43, No. 10
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Griffiths, T. A., Rioux, K., De Buck, J. (2008). Sequence Polymorphisms in a Surface PPE Protein Distinguish Types I, II, and III of Mycobacterium avium subsp. paratuberculosis. J. Clin. Microbiol. 46: 1207-1212 [Abstract] [Full Text]  
  • McCammon, M. T., Gillette, J. S., Thomas, D. P., Ramaswamy, S. V., Graviss, E. A., Kreiswirth, B. N., Vijg, J., Quitugua, T. N. (2005). Detection of rpoB Mutations Associated with Rifampin Resistance in Mycobacterium tuberculosis Using Denaturing Gradient Gel Electrophoresis. Antimicrob. Agents Chemother. 49: 2200-2209 [Abstract] [Full Text]  
  • McCammon, M. T., Gillette, J. S., Thomas, D. P., Ramaswamy, S. V., Rosas, I. I., Graviss, E. A., Vijg, J., Quitugua, T. N. (2005). Detection by Denaturing Gradient Gel Electrophoresis of pncA Mutations Associated with Pyrazinamide Resistance in Mycobacterium tuberculosis Isolates from the United States-Mexico Border Region. Antimicrob. Agents Chemother. 49: 2210-2217 [Abstract] [Full Text]  
  • Zingale, A., Carrera, P., Lazzarin, A., Scarpellini, P. (2003). Detection of Pneumocystis carinii and Characterization of Mutations Associated with Sulfa Resistance in Bronchoalveolar Lavage Samples from Human Immunodeficiency Virus-Infected Subjects. J. Clin. Microbiol. 41: 2709-2712 [Abstract] [Full Text]  
  • Van Der Zanden, A. G. M., Te Koppele-Vije, E. M., Vijaya Bhanu, N., Van Soolingen, D., Schouls, L. M. (2003). Use of DNA Extracts from Ziehl-Neelsen-Stained Slides for Molecular Detection of Rifampin Resistance and Spoligotyping of Mycobacterium tuberculosis. J. Clin. Microbiol. 41: 1101-1108 [Abstract] [Full Text]  
  • Scarpellini, P., Carrera, P., Cavallero, A., Cernuschi, M., Mezzi, G., Testoni, P. A., Zingale, A., Lazzarin, A. (2002). Direct Detection of Helicobacter pylori Mutations Associated with Macrolide Resistance in Gastric Biopsy Material Taken from Human Immunodeficiency Virus-Infected Subjects. J. Clin. Microbiol. 40: 2234-2237 [Abstract] [Full Text]  
  • Garcia de Viedma, D., del Sol Diaz Infantes, M., Lasala, F., Chaves, F., Alcala, L., Bouza, E. (2002). New Real-Time PCR Able To Detect in a Single Tube Multiple Rifampin Resistance Mutations and High-Level Isoniazid Resistance Mutations in Mycobacterium tuberculosis. J. Clin. Microbiol. 40: 988-995 [Abstract] [Full Text]  
  • Fluit, A. C., Visser, M. R., Schmitz, F.-J. (2001). Molecular Detection of Antimicrobial Resistance. Clin. Microbiol. Rev. 14: 836-871 [Abstract] [Full Text]  
  • Garcia, L., Alonso-Sanz, M., Rebollo, M. J., Tercero, J. C., Chaves, F. (2001). Mutations in the rpoB Gene of Rifampin-Resistant Mycobacterium tuberculosis Isolates in Spain and Their Rapid Detection by PCR-Enzyme-Linked Immunosorbent Assay. J. Clin. Microbiol. 39: 1813-1818 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»