This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Aires, J. R.
Right arrow Articles by Plésiat, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aires, J. R.
Right arrow Articles by Plésiat, P.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, November 1999, p. 2624-2628, Vol. 43, No. 11
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Involvement of an Active Efflux System in the Natural Resistance of Pseudomonas aeruginosa to Aminoglycosides

Julio Ramos Aires,1 Thilo Köhler,2 Hiroshi Nikaido,3 and Patrick Plésiat1,*

Department of Bacteriology, Centre Hospitalier Universitaire, F-25030 Besançon, France1; Department of Genetics and Microbiology, Centre Médical Universitaire, Geneva, Switzerland2; and Department of Molecular and Cell Biology, University of California, Berkeley, California 947203

Received 26 April 1999/Returned for modification 18 June 1999/Accepted 18 August 1999

A mutant, named 11B, hypersusceptible to aminoglycosides, tetracycline, and erythromycin was isolated after Tn501 insertion mutagenesis of Pseudomonas aeruginosa PAO1. Cloning and sequencing experiments showed that 11B was deficient in an, at that time, unknown active efflux system that contains homologs of MexAB. This locus also contained a putative regulatory gene, mexZ, transcribed divergently from the efflux operon. Introduction of a recombinant plasmid that carries the genes of the efflux system restored the resistance of 11B to parental levels, whereas overexpression of these genes strongly increased the MICs of substrate antibiotics for the PAO1 host. Antibiotic accumulation studies confirmed that this new system is an energy-dependent active efflux system that pumps out aminoglycosides. Furthermore, this system appeared to function with an outer membrane protein, OprM. While the present paper was being written and reviewed, genes with a sequence identical to our pump genes, mexXY of P. aeruginosa, have been reported to increase resistance to erythromycin, fluoroquinolones, and organic cations in Escherichia coli hosts, although efflux of aminoglycosides was not examined (Mine et al., Antimicrob. Agents Chemother. 43:415-417, 1999). Our study thus shows that the MexXY system plays an important role in the intrinsic resistance of P. aeruginosa to aminoglycosides. Although overexpression of MexXY increased the level of resistance to fluoroquinolones, disruption of the mexXY operon in P. aeruginosa had no detectable effect on susceptibility to these agents.

* Corresponding author. Mailing address: Laboratoire de Bactériologie, Hôpital Jean Minjoz, 25030 Besançon cedex, France. Phone: (33) 3 81 66 82 86. Fax: (33) 3 81 66 89 14. E-mail: patrick.plesiat{at}ufc-chu.univ-fcomte.fr.

Antimicrobial Agents and Chemotherapy, November 1999, p. 2624-2628, Vol. 43, No. 11
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Rodriguez-Martinez, J.-M., Poirel, L., Nordmann, P. (2009). Molecular Epidemiology and Mechanisms of Carbapenem Resistance in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 53: 4783-4788 [Abstract] [Full Text]  
  • Mima, T., Kohira, N., Li, Y., Sekiya, H., Ogawa, W., Kuroda, T., Tsuchiya, T. (2009). Gene cloning and characteristics of the RND-type multidrug efflux pump MuxABC-OpmB possessing two RND components in Pseudomonas aeruginosa. Microbiology 155: 3509-3517 [Abstract] [Full Text]  
  • Lister, P. D., Wolter, D. J., Hanson, N. D. (2009). Antibacterial-Resistant Pseudomonas aeruginosa: Clinical Impact and Complex Regulation of Chromosomally Encoded Resistance Mechanisms. Clin. Microbiol. Rev. 22: 582-610 [Abstract] [Full Text]  
  • Yamamoto, M., Ueda, A., Kudo, M., Matsuo, Y., Fukushima, J., Nakae, T., Kaneko, T., Ishigatsubo, Y. (2009). Role of MexZ and PA5471 in transcriptional regulation of mexXY in Pseudomonas aeruginosa. Microbiology 155: 3312-3321 [Abstract] [Full Text]  
  • Pena, C., Suarez, C., Tubau, F., Juan, C., Moya, B., Dominguez, M. A., Oliver, A., Pujol, M., Ariza, J. (2009). Nosocomial Outbreak of a Non-Cefepime-Susceptible Ceftazidime-Susceptible Pseudomonas aeruginosa Strain Overexpressing MexXY-OprM and Producing an Integron-Borne PSE-1 ss-Lactamase. J. Clin. Microbiol. 47: 2381-2387 [Abstract] [Full Text]  
  • Baum, E. Z., Crespo-Carbone, S. M., Morrow, B. J., Davies, T. A., Foleno, B. D., He, W., Queenan, A. M., Bush, K. (2009). Effect of MexXY Overexpression on Ceftobiprole Susceptibility in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 53: 2785-2790 [Abstract] [Full Text]  
  • Vettoretti, L., Plesiat, P., Muller, C., El Garch, F., Phan, G., Attree, I., Ducruix, A., Llanes, C. (2009). Efflux Unbalance in Pseudomonas aeruginosa Isolates from Cystic Fibrosis Patients. Antimicrob. Agents Chemother. 53: 1987-1997 [Abstract] [Full Text]  
  • Holden, M. T. G., Seth-Smith, H. M. B., Crossman, L. C., Sebaihia, M., Bentley, S. D., Cerdeno-Tarraga, A. M., Thomson, N. R., Bason, N., Quail, M. A., Sharp, S., Cherevach, I., Churcher, C., Goodhead, I., Hauser, H., Holroyd, N., Mungall, K., Scott, P., Walker, D., White, B., Rose, H., Iversen, P., Mil-Homens, D., Rocha, E. P. C., Fialho, A. M., Baldwin, A., Dowson, C., Barrell, B. G., Govan, J. R., Vandamme, P., Hart, C. A., Mahenthiralingam, E., Parkhill, J. (2009). The Genome of Burkholderia cenocepacia J2315, an Epidemic Pathogen of Cystic Fibrosis Patients. J. Bacteriol. 191: 261-277 [Abstract] [Full Text]  
  • Kvist, M., Hancock, V., Klemm, P. (2008). Inactivation of Efflux Pumps Abolishes Bacterial Biofilm Formation. Appl. Environ. Microbiol. 74: 7376-7382 [Abstract] [Full Text]  
  • Cerda-Maira, F. A., Ringelberg, C. S., Taylor, R. K. (2008). The Bile Response Repressor BreR Regulates Expression of the Vibrio cholerae breAB Efflux System Operon. J. Bacteriol. 190: 7441-7452 [Abstract] [Full Text]  
  • Jeannot, K., Elsen, S., Kohler, T., Attree, I., van Delden, C., Plesiat, P. (2008). Resistance and Virulence of Pseudomonas aeruginosa Clinical Strains Overproducing the MexCD-OprJ Efflux Pump. Antimicrob. Agents Chemother. 52: 2455-2462 [Abstract] [Full Text]  
  • Zhang, L., Mah, T.-F. (2008). Involvement of a Novel Efflux System in Biofilm-Specific Resistance to Antibiotics. J. Bacteriol. 190: 4447-4452 [Abstract] [Full Text]  
  • Hocquet, D., Muller, A., Blanc, K., Plesiat, P., Talon, D., Monnet, D. L., Bertrand, X. (2008). Relationship between Antibiotic Use and Incidence of MexXY-OprM Overproducers among Clinical Isolates of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 52: 1173-1175 [Abstract] [Full Text]  
  • Kohler, T., Dumas, J.-L., Van Delden, C. (2007). Ribosome Protection Prevents Azithromycin-Mediated Quorum-Sensing Modulation and Stationary-Phase Killing of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 51: 4243-4248 [Abstract] [Full Text]  
  • Stavri, M., Piddock, L. J. V., Gibbons, S. (2007). Bacterial efflux pump inhibitors from natural sources. J Antimicrob Chemother 59: 1247-1260 [Abstract] [Full Text]  
  • El'Garch, F., Jeannot, K., Hocquet, D., Llanes-Barakat, C., Plesiat, P. (2007). Cumulative Effects of Several Nonenzymatic Mechanisms on the Resistance of Pseudomonas aeruginosa to Aminoglycosides. Antimicrob. Agents Chemother. 51: 1016-1021 [Abstract] [Full Text]  
  • Marr, A. K., Overhage, J., Bains, M., Hancock, R. E. W. (2007). The Lon protease of Pseudomonas aeruginosa is induced by aminoglycosides and is involved in biofilm formation and motility. Microbiology 153: 474-482 [Abstract] [Full Text]  
  • Depardieu, F., Podglajen, I., Leclercq, R., Collatz, E., Courvalin, P. (2007). Modes and Modulations of Antibiotic Resistance Gene Expression. Clin. Microbiol. Rev. 20: 79-114 [Abstract] [Full Text]  
  • Vediyappan, G., Borisova, T., Fralick, J. A. (2006). Isolation and Characterization of VceC Gain-of-Function Mutants That Can Function with the AcrAB Multiple-Drug-Resistant Efflux Pump of Escherichia coli. J. Bacteriol. 188: 3757-3762 [Abstract] [Full Text]  
  • Smith, E. E., Buckley, D. G., Wu, Z., Saenphimmachak, C., Hoffman, L. R., D'Argenio, D. A., Miller, S. I., Ramsey, B. W., Speert, D. P., Moskowitz, S. M., Burns, J. L., Kaul, R., Olson, M. V. (2006). From the Cover: Genetic adaptation by Pseudomonas aeruginosa to the airways of cystic fibrosis patients. Proc. Natl. Acad. Sci. USA 103: 8487-8492 [Abstract] [Full Text]  
  • Griffith, D. C., Corcoran, E., Lofland, D., Lee, A., Cho, D., Lomovskaya, O., Dudley, M. N. (2006). Pharmacodynamics of Levofloxacin against Pseudomonas aeruginosa with Reduced Susceptibility Due to Different Efflux Pumps: Do Elevated MICs Always Predict Reduced In Vivo Efficacy?. Antimicrob. Agents Chemother. 50: 1628-1632 [Abstract] [Full Text]  
  • Quale, J., Bratu, S., Gupta, J., Landman, D. (2006). Interplay of Efflux System, ampC, and oprD Expression in Carbapenem Resistance of Pseudomonas aeruginosa Clinical Isolates.. Antimicrob. Agents Chemother. 50: 1633-1641 [Abstract] [Full Text]  
  • Piddock, L. J. V. (2006). Clinically Relevant Chromosomally Encoded Multidrug Resistance Efflux Pumps in Bacteria. Clin. Microbiol. Rev. 19: 382-402 [Abstract] [Full Text]  
  • Hocquet, D., Nordmann, P., El Garch, F., Cabanne, L., Plesiat, P. (2006). Involvement of the MexXY-OprM Efflux System in Emergence of Cefepime Resistance in Clinical Strains of Pseudomonas aeruginosa.. Antimicrob. Agents Chemother. 50: 1347-1351 [Abstract] [Full Text]  
  • Morita, Y., Sobel, M. L., Poole, K. (2006). Antibiotic Inducibility of the MexXY Multidrug Efflux System of Pseudomonas aeruginosa: Involvement of the Antibiotic-Inducible PA5471 Gene Product.. J. Bacteriol. 188: 1847-1855 [Abstract] [Full Text]  
  • Hearn, E. M., Gray, M. R., Foght, J. M. (2006). Mutations in the Central Cavity and Periplasmic Domain Affect Efflux Activity of the Resistance-Nodulation-Division Pump EmhB from Pseudomonas fluorescens cLP6a. J. Bacteriol. 188: 115-123 [Abstract] [Full Text]  
  • Kang, H., Gross, D. C. (2005). Characterization of a Resistance-Nodulation-Cell Division Transporter System Associated with the syr-syp Genomic Island of Pseudomonas syringae pv. syringae. Appl. Environ. Microbiol. 71: 5056-5065 [Abstract] [Full Text]  
  • Jeannot, K., Sobel, M. L., El Garch, F., Poole, K., Plesiat, P. (2005). Induction of the MexXY Efflux Pump in Pseudomonas aeruginosa Is Dependent on Drug-Ribosome Interaction. J. Bacteriol. 187: 5341-5346 [Abstract] [Full Text]  
  • Chuanchuen, R., Gaynor, J. B., Karkhoff-Schweizer, R., Schweizer, H. P. (2005). Molecular Characterization of MexL, the Transcriptional Repressor of the mexJK Multidrug Efflux Operon in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 49: 1844-1851 [Abstract] [Full Text]  
  • Sobel, M. L., Neshat, S., Poole, K. (2005). Mutations in PA2491 (mexS) Promote MexT-Dependent mexEF-oprN Expression and Multidrug Resistance in a Clinical Strain of Pseudomonas aeruginosa. J. Bacteriol. 187: 1246-1253 [Abstract] [Full Text]  
  • Poole, K. (2005). Aminoglycoside Resistance in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 49: 479-487 [Full Text]  
  • Marchand, I., Damier-Piolle, L., Courvalin, P., Lambert, T. (2004). Expression of the RND-Type Efflux Pump AdeABC in Acinetobacter baumannii Is Regulated by the AdeRS Two-Component System. Antimicrob. Agents Chemother. 48: 3298-3304 [Abstract] [Full Text]  
  • Hansen, L. H., Johannesen, E., Burmolle, M., Sorensen, A. H., Sorensen, S. J. (2004). Plasmid-Encoded Multidrug Efflux Pump Conferring Resistance to Olaquindox in Escherichia coli. Antimicrob. Agents Chemother. 48: 3332-3337 [Abstract] [Full Text]  
  • Nehme, D., Li, X.-Z., Elliot, R., Poole, K. (2004). Assembly of the MexAB-OprM Multidrug Efflux System of Pseudomonas aeruginosa: Identification and Characterization of Mutations in mexA Compromising MexA Multimerization and Interaction with MexB. J. Bacteriol. 186: 2973-2983 [Abstract] [Full Text]  
  • Vogne, C., Aires, J. R., Bailly, C., Hocquet, D., Plesiat, P. (2004). Role of the Multidrug Efflux System MexXY in the Emergence of Moderate Resistance to Aminoglycosides among Pseudomonas aeruginosa Isolates from Patients with Cystic Fibrosis. Antimicrob. Agents Chemother. 48: 1676-1680 [Abstract] [Full Text]  
  • Llanes, C., Hocquet, D., Vogne, C., Benali-Baitich, D., Neuwirth, C., Plesiat, P. (2004). Clinical Strains of Pseudomonas aeruginosa Overproducing MexAB-OprM and MexXY Efflux Pumps Simultaneously. Antimicrob. Agents Chemother. 48: 1797-1802 [Abstract] [Full Text]  
  • Maseda, H., Sawada, I., Saito, K., Uchiyama, H., Nakae, T., Nomura, N. (2004). Enhancement of the mexAB-oprM Efflux Pump Expression by a Quorum-Sensing Autoinducer and Its Cancellation by a Regulator, MexT, of the mexEF-oprN Efflux Pump Operon in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 48: 1320-1328 [Abstract] [Full Text]  
  • Perron, K., Caille, O., Rossier, C., van Delden, C., Dumas, J.-L., Kohler, T. (2004). CzcR-CzcS, a Two-component System Involved in Heavy Metal and Carbapenem Resistance in Pseudomonas aeruginosa. J. Biol. Chem. 279: 8761-8768 [Abstract] [Full Text]  
  • Ramos-Aires, J., Plesiat, P., Kocjancic-Curty, L., Kohler, T. (2004). Selection of an Antibiotic-Hypersusceptible Mutant of Pseudomonas aeruginosa: Identification of the GlmR Transcriptional Regulator. Antimicrob. Agents Chemother. 48: 843-851 [Abstract] [Full Text]  
  • Middlemiss, J. K., Poole, K. (2004). Differential Impact of MexB Mutations on Substrate Selectivity of the MexAB-OprM Multidrug Efflux Pump of Pseudomonas aeruginosa. J. Bacteriol. 186: 1258-1269 [Abstract] [Full Text]  
  • Sobel, M. L., McKay, G. A., Poole, K. (2003). Contribution of the MexXY Multidrug Transporter to Aminoglycoside Resistance in Pseudomonas aeruginosa Clinical Isolates. Antimicrob. Agents Chemother. 47: 3202-3207 [Abstract] [Full Text]  
  • Yu, E. W., Aires, J. R., Nikaido, H. (2003). AcrB Multidrug Efflux Pump of Escherichia coli: Composite Substrate-Binding Cavity of Exceptional Flexibility Generates Its Extremely Wide Substrate Specificity. J. Bacteriol. 185: 5657-5664 [Full Text]  
  • Vakulenko, S. B., Mobashery, S. (2003). Versatility of Aminoglycosides and Prospects for Their Future. Clin. Microbiol. Rev. 16: 430-450 [Abstract] [Full Text]  
  • Hocquet, D., Bertrand, X., Kohler, T., Talon, D., Plesiat, P. (2003). Genetic and Phenotypic Variations of a Resistant Pseudomonas aeruginosa Epidemic Clone. Antimicrob. Agents Chemother. 47: 1887-1894 [Abstract] [Full Text]  
  • Magnet, S., Smith, T.-A., Zheng, R., Nordmann, P., Blanchard, J. S. (2003). Aminoglycoside Resistance Resulting from Tight Drug Binding to an Altered Aminoglycoside Acetyltransferase. Antimicrob. Agents Chemother. 47: 1577-1583 [Abstract] [Full Text]  
  • Sabtcheva, S., Galimand, M., Gerbaud, G., Courvalin, P., Lambert, T. (2003). Aminoglycoside Resistance Gene ant(4')-IIb of Pseudomonas aeruginosa BM4492, a Clinical Isolate from Bulgaria. Antimicrob. Agents Chemother. 47: 1584-1588 [Abstract] [Full Text]  
  • Van Bambeke, F., Glupczynski, Y., Plesiat, P., Pechere, J. C., Tulkens, P. M. (2003). Antibiotic efflux pumps in prokaryotic cells: occurrence, impact on resistance and strategies for the future of antimicrobial therapy. J Antimicrob Chemother 51: 1055-1065 [Full Text]  
  • Hocquet, D., Vogne, C., El Garch, F., Vejux, A., Gotoh, N., Lee, A., Lomovskaya, O., Plesiat, P. (2003). MexXY-OprM Efflux Pump Is Necessary for Adaptive Resistance of Pseudomonas aeruginosa to Aminoglycosides. Antimicrob. Agents Chemother. 47: 1371-1375 [Abstract] [Full Text]  
  • Li, X.-Z., Zhang, L., McKay, G. A., Poole, K. (2003). Role of the acetyltransferase AAC(6')-Iz modifying enzyme in aminoglycoside resistance in Stenotrophomonas maltophilia. J Antimicrob Chemother 51: 803-811 [Abstract] [Full Text]  
  • Dean, C. R., Visalli, M. A., Projan, S. J., Sum, P.-E., Bradford, P. A. (2003). Efflux-Mediated Resistance to Tigecycline (GAR-936) in Pseudomonas aeruginosa PAO1. Antimicrob. Agents Chemother. 47: 972-978 [Abstract] [Full Text]  
  • Jo, J. T. H., Brinkman, F. S. L., Hancock, R. E. W. (2003). Aminoglycoside Efflux in Pseudomonas aeruginosa: Involvement of Novel Outer Membrane Proteins. Antimicrob. Agents Chemother. 47: 1101-1111 [Abstract] [Full Text]  
  • Li, X.-Z., Poole, K., Nikaido, H. (2003). Contributions of MexAB-OprM and an EmrE Homolog to Intrinsic Resistance of Pseudomonas aeruginosa to Aminoglycosides and Dyes. Antimicrob. Agents Chemother. 47: 27-33 [Abstract] [Full Text]  
  • Grkovic, S., Brown, M. H., Skurray, R. A. (2002). Regulation of Bacterial Drug Export Systems. Microbiol. Mol. Biol. Rev. 66: 671-701 [Abstract] [Full Text]  
  • Tikhonova, E. B., Wang, Q., Zgurskaya, H. I. (2002). Chimeric Analysis of the Multicomponent Multidrug Efflux Transporters from Gram-Negative Bacteria. J. Bacteriol. 184: 6499-6507 [Abstract] [Full Text]  
  • Chuanchuen, R., Narasaki, C. T., Schweizer, H. P. (2002). The MexJK Efflux Pump of Pseudomonas aeruginosa Requires OprM for Antibiotic Efflux but Not for Efflux of Triclosan. J. Bacteriol. 184: 5036-5044 [Abstract] [Full Text]  
  • Okamoto, K., Gotoh, N., Nishino, T. (2002). Extrusion of Penem Antibiotics by Multicomponent Efflux Systems MexAB-OprM, MexCD-OprJ, and MexXY-OprM of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 46: 2696-2699 [Abstract] [Full Text]  
  • Aires, J. R., Pechere, J.-C., Van Delden, C., Kohler, T. (2002). Amino Acid Residues Essential for Function of the MexF Efflux Pump Protein of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 46: 2169-2173 [Abstract] [Full Text]  
  • Hirakata, Y., Srikumar, R., Poole, K., Gotoh, N., Suematsu, T., Kohno, S., Kamihira, S., Hancock, R. E. W., Speert, D. P. (2002). Multidrug Efflux Systems Play an Important Role in the Invasiveness of Pseudomonas aeruginosa. JEM 196: 109-118 [Abstract] [Full Text]  
  • Raherison, S., Gonzalez, P., Renaudin, H., Charron, A., Bebear, C., Bebear, C. M. (2002). Evidence of Active Efflux in Resistance to Ciprofloxacin and to Ethidium Bromide by Mycoplasma hominis. Antimicrob. Agents Chemother. 46: 672-679 [Abstract] [Full Text]  
  • Li, X.-Z., Zhang, L., Poole, K. (2002). SmeC, an Outer Membrane Multidrug Efflux Protein of Stenotrophomonas maltophilia. Antimicrob. Agents Chemother. 46: 333-343 [Abstract] [Full Text]  
  • Magnet, S., Courvalin, P., Lambert, T. (2001). Resistance-Nodulation-Cell Division-Type Efflux Pump Involved in Aminoglycoside Resistance in Acinetobacter baumannii Strain BM4454. Antimicrob. Agents Chemother. 45: 3375-3380 [Abstract] [Full Text]  
  • Zhang, L., Li, X.-Z., Poole, K. (2001). SmeDEF Multidrug Efflux Pump Contributes to Intrinsic Multidrug Resistance in Stenotrophomonas maltophilia. Antimicrob. Agents Chemother. 45: 3497-3503 [Abstract] [Full Text]  
  • Kohler, T., van Delden, C., Curty, L. K., Hamzehpour, M. M., Pechere, J.-C. (2001). Overexpression of the MexEF-OprN Multidrug Efflux System Affects Cell-to-Cell Signaling in Pseudomonas aeruginosa. J. Bacteriol. 183: 5213-5222 [Abstract] [Full Text]  
  • Okamoto, K., Gotoh, N., Nishino, T. (2001). Pseudomonas aeruginosa Reveals High Intrinsic Resistance to Penem Antibiotics: Penem Resistance Mechanisms and Their Interplay. Antimicrob. Agents Chemother. 45: 1964-1971 [Abstract] [Full Text]  
  • Mao, W., Warren, M. S., Lee, A., Mistry, A., Lomovskaya, O. (2001). MexXY-OprM Efflux Pump Is Required for Antagonism of Aminoglycosides by Divalent Cations in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 45: 2001-2007 [Abstract] [Full Text]  
  • De Kievit, T. R., Parkins, M. D., Gillis, R. J., Srikumar, R., Ceri, H., Poole, K., Iglewski, B. H., Storey, D. G. (2001). Multidrug Efflux Pumps: Expression Patterns and Contribution to Antibiotic Resistance in Pseudomonas aeruginosa Biofilms. Antimicrob. Agents Chemother. 45: 1761-1770 [Abstract] [Full Text]  
  • Masuda, N., Sakagawa, E., Ohya, S., Gotoh, N., Nishino, T. (2001). Hypersusceptibility of the Pseudomonas aeruginosa nfxB Mutant to {beta}-Lactams Due to Reduced Expression of the AmpC {beta}-Lactamase. Antimicrob. Agents Chemother. 45: 1284-1286 [Abstract] [Full Text]  
  • Chuanchuen, R., Beinlich, K., Hoang, T. T., Becher, A., Karkhoff-Schweizer, R. R., Schweizer, H. P. (2001). Cross-Resistance between Triclosan and Antibiotics in Pseudomonas aeruginosa Is Mediated by Multidrug Efflux Pumps: Exposure of a Susceptible Mutant Strain to Triclosan Selects nfxB Mutants Overexpressing MexCD-OprJ. Antimicrob. Agents Chemother. 45: 428-432 [Abstract] [Full Text]  
  • Join-Lambert, O. F., Michéa-Hamzehpour, M., Köhler, T., Chau, F., Faurisson, F., Dautrey, S., Vissuzaine, C., Carbon, C., Pechère, J.-C. (2001). Differential Selection of Multidrug Efflux Mutants by Trovafloxacin and Ciprofloxacin in an Experimental Model of Pseudomonas aeruginosa Acute Pneumonia in Rats. Antimicrob. Agents Chemother. 45: 571-576 [Abstract] [Full Text]  
  • Evans, K., Adewoye, L., Poole, K. (2001). MexR Repressor of the mexAB-oprM Multidrug Efflux Operon of Pseudomonas aeruginosa: Identification of MexR Binding Sites in the mexA-mexR Intergenic Region. J. Bacteriol. 183: 807-812 [Abstract] [Full Text]  
  • Li, X.-Z., Poole, K. (2001). Mutational Analysis of the OprM Outer Membrane Component of the MexA-MexB-OprM Multidrug Efflux System of Pseudomonas aeruginosa. J. Bacteriol. 183: 12-27 [Abstract] [Full Text]  
  • Lomovskaya, O., Warren, M. S., Lee, A., Galazzo, J., Fronko, R., Lee, M., Blais, J., Cho, D., Chamberland, S., Renau, T., Leger, R., Hecker, S., Watkins, W., Hoshino, K., Ishida, H., Lee, V. J. (2001). Identification and Characterization of Inhibitors of Multidrug Resistance Efflux Pumps in Pseudomonas aeruginosa: Novel Agents for Combination Therapy. Antimicrob. Agents Chemother. 45: 105-116 [Abstract] [Full Text]  
  • Wong, K. K. Y., Brinkman, F. S. L., Benz, R. S., Hancock, R. E. W. (2001). Evaluation of a Structural Model of Pseudomonas aeruginosa Outer Membrane Protein OprM, an Efflux Component Involved in Intrinsic Antibiotic Resistance. J. Bacteriol. 183: 367-374 [Abstract] [Full Text]  
  • Li, X.-Z., Barre, N., Poole, K. (2000). Influence of the MexA-MexB-OprM multidrug efflux system on expression of the MexC-MexD-OprJ and MexE-MexF-OprN multidrug efflux systems in Pseudomonas aeruginosa. J Antimicrob Chemother 46: 885-893 [Abstract] [Full Text]  
  • Putman, M., van Veen, H. W., Konings, W. N. (2000). Molecular Properties of Bacterial Multidrug Transporters. Microbiol. Mol. Biol. Rev. 64: 672-693 [Abstract] [Full Text]  
  • Masuda, N., Sakagawa, E., Ohya, S., Gotoh, N., Tsujimoto, H., Nishino, T. (2000). Substrate Specificities of MexAB-OprM, MexCD-OprJ, and MexXY-OprM Efflux Pumps in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 44: 3322-3327 [Abstract] [Full Text]  
  • Alonso, A., Martínez, J. L. (2000). Cloning and Characterization of SmeDEF, a Novel Multidrug Efflux Pump from Stenotrophomonas maltophilia. Antimicrob. Agents Chemother. 44: 3079-3086 [Abstract] [Full Text]  
  • Pumbwe, L., Piddock, L. J. V. (2000). Two Efflux Systems Expressed Simultaneously in Multidrug-Resistant Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 44: 2861-2864 [Abstract] [Full Text]  
  • Poole, K. (2000). Efflux-Mediated Resistance to Fluoroquinolones in Gram-Negative Bacteria. Antimicrob. Agents Chemother. 44: 2233-2241 [Full Text]  
  • Masuda, N., Sakagawa, E., Ohya, S., Gotoh, N., Tsujimoto, H., Nishino, T. (2000). Contribution of the MexX-MexY-OprM Efflux System to Intrinsic Resistance in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 44: 2242-2246 [Abstract] [Full Text]  
  • Lee, A., Mao, W., Warren, M. S., Mistry, A., Hoshino, K., Okumura, R., Ishida, H., Lomovskaya, O. (2000). Interplay between Efflux Pumps May Provide Either Additive or Multiplicative Effects on Drug Resistance. J. Bacteriol. 182: 3142-3150 [Abstract] [Full Text]  
  • Mazzariol, A., Cornaglia, G., Nikaido, H. (2000). Contributions of the AmpC beta -Lactamase and the AcrAB Multidrug Efflux System in Intrinsic Resistance of Escherichia coli K-12 to beta -Lactams. Antimicrob. Agents Chemother. 44: 1387-1390 [Abstract] [Full Text]  
  • Rosenberg, E. Y., Ma, D., Nikaido, H. (2000). AcrD of Escherichia coli Is an Aminoglycoside Efflux Pump. J. Bacteriol. 182: 1754-1756 [Abstract] [Full Text]  
  • Brooun, A., Liu, S., Lewis, K. (2000). A Dose-Response Study of Antibiotic Resistance in Pseudomonas aeruginosa Biofilms. Antimicrob. Agents Chemother. 44: 640-646 [Abstract] [Full Text]  
  • Srikumar, R., Paul, C. J., Poole, K. (2000). Influence of Mutations in the mexR Repressor Gene on Expression of the MexA-MexB-OprM Multidrug Efflux System of Pseudomonas aeruginosa. J. Bacteriol. 182: 1410-1414 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»