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Antimicrobial Agents and Chemotherapy, November 1999, p. 2798-2800, Vol. 43, No. 11
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Overexpression of Erg11p by the Regulatable GAL1 Promoter Confers Fluconazole Resistance in Saccharomyces cerevisiae

Dimitrios P. Kontoyiannis,1,* Namita Sagar,1 and Kendal D. Hirschi2

Department of Internal Medicine Specialties, Section of Infectious Diseases, The University of Texas M. D. Anderson Cancer Center,1 and USDA Agricultural Research Service and Children's Nutrition Research Center and Department of Pediatrics, Baylor College of Medicine,2 Houston, Texas

Received 1 June 1999/Returned for modification 17 August 1999/Accepted 7 September 1999

The contribution of the dosage of target enzyme P-450 14alpha -demethylase (14alpha DM) to fluconazole resistance in both Candida albicans and Saccharomyces cerevisiae remains unclear. Here, we show that overexpression of Saccharomyces P-450 14alpha DM in S. cerevisiae, under the control of the regulatable promoter GAL1, results in azole resistance.

* Corresponding author. Mailing address: Section of Infectious Diseases, Department of Internal Medicine Specialties, 1515 Holcombe Blvd., Box 47, Houston, TX 77030. Phone: (713) 792-6237. Fax: (713) 794-4351. E-mail: dkontoyi{at}notes.mdacc.tmc.edu.

Antimicrobial Agents and Chemotherapy, November 1999, p. 2798-2800, Vol. 43, No. 11
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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