This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cowen, L. E.
Right arrow Articles by Anderson, J. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cowen, L. E.
Right arrow Articles by Anderson, J. B.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, December 1999, p. 2930-2938, Vol. 43, No. 12
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Multilocus Genotypes and DNA Fingerprints Do Not Predict Variation in Azole Resistance among Clinical Isolates of Candida albicans

Leah E. Cowen,1,* Caroline Sirjusingh,1 Richard C. Summerbell,2,3 Sharon Walmsley,4 Susan Richardson,5 Linda M. Kohn,1 and James B. Anderson1

Department of Botany, University of Toronto, Mississauga, Ontario, Canada L5L 1C61; Mycology Laboratory, Ontario Ministry of Health, Laboratories Branch, Toronto, Ontario, Canada M5W 1R52; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada M5G 1L53; Department of Medicine, Division of Infectious Disease, The Toronto Hospital, University of Toronto, Toronto, Ontario, Canada M5G 2C44; Department of Pediatric Laboratory Medicine---Microbiology, The Hospital for Sick Children, Toronto, Ontario, Canada M5G 1X85

Received 18 May 1999/Returned for modification 17 August 1999/Accepted 1 October 1999

If variation in azole resistance is due to inherent differences in strains of Candida albicans, as a predominantly clonal organism, then correlation between multilocus genotypes and drug resistance would be expected. A sample of 81 clinical isolates from patients infected with human immunodeficiency virus in Toronto, Canada, plus 3 reference isolates were genotyped at 16 loci, distributed on all linkage groups, by means of oligonucleotide hybridizations specific for each of the alleles at each locus. These multilocus genotypes were significantly correlated with DNA fingerprints obtained with the species-specific probe 27A, indicating widespread linkage disequilibrium in the genome. There were 64 multilocus diploid genotypes and 77 DNA fingerprint types delineated in this sample. Neither the multilocus genotyping nor DNA fingerprinting alone identified all of the 81 types identified by the combination of these two methods. Multilocus genotypes were not predictive of fluconazole resistance, suggesting that resistance is gained or lost too quickly to be predicted by linkage with neutral markers.

* Corresponding author. Mailing address: Department of Botany, University of Toronto at Mississauga, 3359 Mississauga Rd. North, Mississauga, Ontario, Canada L5L 1C6. Phone: (905) 828-5338. Fax: (905) 828-3792. E-mail: lcowen{at}credit.erin.utoronto.ca

Antimicrobial Agents and Chemotherapy, December 1999, p. 2930-2938, Vol. 43, No. 12
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Forche, A., Magee, P. T., Selmecki, A., Berman, J., May, G. (2009). Evolution in Candida albicans Populations During a Single Passage Through a Mouse Host. Genetics 182: 799-811 [Abstract] [Full Text]  
  • Trofa, D., Gacser, A., Nosanchuk, J. D. (2008). Candida parapsilosis, an Emerging Fungal Pathogen. Clin. Microbiol. Rev. 21: 606-625 [Abstract] [Full Text]  
  • Odds, F. C., Jacobsen, M. D. (2008). Multilocus Sequence Typing of Pathogenic Candida Species. Eukaryot Cell 7: 1075-1084 [Full Text]  
  • Anderson, J. B., Ricker, N., Sirjusingh, C. (2006). Antagonism between Two Mechanisms of Antifungal Drug Resistance.. Eukaryot Cell 5: 1243-1251 [Abstract] [Full Text]  
  • Forche, A., Magee, P. T., Magee, B. B., May, G. (2004). Genome-Wide Single-Nucleotide Polymorphism Map for Candida albicans. Eukaryot Cell 3: 705-714 [Abstract] [Full Text]  
  • Pujol, C., Pfaller, M. A., Soll, D. R. (2004). Flucytosine Resistance Is Restricted to a Single Genetic Clade of Candida albicans. Antimicrob. Agents Chemother. 48: 262-266 [Abstract] [Full Text]  
  • Li, X., Yan, Z., Xu, J. (2003). Quantitative variation of biofilms among strains in natural populations of Candida albicans. Microbiology 149: 353-362 [Abstract] [Full Text]  
  • Cowen, L. E., Nantel, A., Whiteway, M. S., Thomas, D. Y., Tessier, D. C., Kohn, L. M., Anderson, J. B. (2002). Population genomics of drug resistance in Candida albicans. Proc. Natl. Acad. Sci. USA 99: 9284-9289 [Abstract] [Full Text]  
  • Bougnoux, M.-E., Morand, S., d'Enfert, C. (2002). Usefulness of Multilocus Sequence Typing for Characterization of Clinical Isolates of Candida albicans. J. Clin. Microbiol. 40: 1290-1297 [Abstract] [Full Text]  
  • Rustad, T. R., Stevens, D. A., Pfaller, M. A., White, T. C. (2002). Homozygosity at the Candida albicans MTL locus associated with azole resistance. Microbiology 148: 1061-1072 [Abstract] [Full Text]  
  • Botterel, F., Desterke, C., Costa, C., Bretagne, S. (2001). Analysis of Microsatellite Markers of Candida albicans Used for Rapid Typing. J. Clin. Microbiol. 39: 4076-4081 [Abstract] [Full Text]  
  • Luu, L. N., Cowen, L. E., Sirjusingh, C., Kohn, L. M., Anderson, J. B. (2001). Multilocus Genotyping Indicates that the Ability To Invade the Bloodstream Is Widespread among Candida albicans Isolates. J. Clin. Microbiol. 39: 1657-1660 [Abstract] [Full Text]  
  • Anderson, J. B., Wickens, C., Khan, M., Cowen, L. E., Federspiel, N., Jones, T., Kohn, L. M. (2001). Infrequent Genetic Exchange and Recombination in the Mitochondrial Genome of Candida albicans. J. Bacteriol. 183: 865-872 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»