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Antimicrobial Agents and Chemotherapy, December 1999, p. 3018-3021, Vol. 43, No. 12
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Genetic Characterization of Antimicrobial Resistance in Canadian Isolates of Salmonella Serovar Typhimurium DT104

Lai-King Ng,* Michael R. Mulvey, Irene Martin, Geoffrey A. Peters, and Wendy Johnson

Bureau of Microbiology, Laboratory Centre for Disease Control, Health Canada, Winnipeg, Manitoba, Canada

Received 22 April 1999/Returned for modification 16 June 1999/Accepted 17 September 1999

PCR was used to identify antibiotic resistance determinants in 31 Canadian Salmonella serovar Typhimurium DT104 isolates. Genes encoding resistance to ampicillin (pse1 or blaP1), chloramphenicol (pasppflo-like), streptomycin-spectinomycin (aadA2), sulfonamide (sulI), and tetracycline [tet(G)] were mapped to a 13-kb region of DNA of one isolate. Two copies of sulI were identified and mapped to the 3' end of either pse1 or aadA2 integrons. The two integrons were separated by the pasppflo-like gene and the tet(G) gene. The kanamycin resistance determinant (aphA-1) was present on a 2.0-MDa plasmid (five isolates) or on the chromosome (three isolates).

* Corresponding author. Mailing address: Gonococcal Infections Section, Bureau of Microbiology, Laboratory Centre for Disease Control, Health Canada, 1015 Arlington St., Room 2380, Winnipeg, Manitoba, Canada R3E 3R2. Phone: (204) 789-2131. Fax: (204) 789-2140. E-mail: Lai_King_Ng{at}hc-sc.gc.ca.

Antimicrobial Agents and Chemotherapy, December 1999, p. 3018-3021, Vol. 43, No. 12
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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