This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Matsumoto, Y.
Right arrow Articles by Inoue, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Matsumoto, Y.
Right arrow Articles by Inoue, M.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, February 1999, p. 307-313, Vol. 43, No. 2
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Characterization of SFO-1, a Plasmid-Mediated Inducible Class A beta -Lactamase from Enterobacter cloacae

Yoshimi Matsumoto1,2,* and Matsuhisa Inoue1

Department of Microbiology, Kitasato University School of Medicine, Sagamihara,1 and Medicinal Biology Research Laboratories, Fujisawa Pharmaceutical Co., Ltd., Osaka,2 Japan

Received 1 April 1998/Returned for modification 23 July 1998/Accepted 24 November 1998

Enterobacter cloacae 8009 produced an inducible class A beta -lactamase which hydrolyzed cefotaxime efficiently. It also hydrolyzed other beta -lactams except cephamycins and carbapenems. The activity was inhibited by clavulanic acid and imipenem. The bla gene was transferable to Escherichia coli by electroporation of plasmid DNA. The molecular mass of the beta -lactamase was 29 kDa and its pI was 7.3. All of these phenotypic characteristics of the enzyme except for inducible production resemble those of some extended-spectrum class A beta -lactamases like FEC-1. The gene encoding this beta -lactamase was cloned and sequenced. The deduced amino acid sequence of the beta -lactamase was homologous to the AmpA sequences of the Serratia fonticola chromosomal enzyme (96%), MEN-1 (78%), Klebsiella oxytoca chromosomal enzymes (77%), TOHO-1 (75%), and FEC-1 (72%). The conserved sequences of class A beta -lactamases, including the S-X(T)-X(S)-K motif, in the active site were all conserved in this enzyme. On the basis of the high degree of homology to the beta -lactamase of S. fonticola, the enzyme was named SFO-1. The ampR gene was located upstream of the ampA gene, and the AmpR sequence of SFO-1 had homology with the AmpR sequences of the chromosomal beta -lactamases from Citrobacter diversus (80%), Proteus vulgaris (68%), and Pseudomonas aeruginosa (60%). SFO-1 was also inducible in E. coli. However, a transformant harboring plasmid without intact ampR produced a small amount of beta -lactamase constitutively, suggesting that AmpR works as an activator of ampA of SFO-1. This is the first report from Japan describing an inducible plasmid-mediated class A beta -lactamase in gram-negative bacteria.

* Corresponding author. Mailing address: Medicinal Biology Research Laboratories, Fujisawa Pharmaceutical Co., Ltd., 1-6, 2-Chome, Kashima, Yodogawa-ku, Osaka, 532 Japan. Phone: (06) 390-1146. Fax: (06) 304-1192. E-mail: yoshimi_matsumoto{at}po.fujisawa.co.jp.

Antimicrobial Agents and Chemotherapy, February 1999, p. 307-313, Vol. 43, No. 2
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Jacoby, G. A. (2006). {beta}-Lactamase Nomenclature.. Antimicrob. Agents Chemother. 50: 1123-1129 [Full Text]  
  • Girlich, D., Poirel, L., Schluter, A., Nordmann, P. (2005). TLA-2, a Novel Ambler Class A Expanded-Spectrum {beta}-Lactamase. Antimicrob. Agents Chemother. 49: 4767-4770 [Abstract] [Full Text]  
  • Paterson, D. L., Bonomo, R. A. (2005). Extended-Spectrum {beta}-Lactamases: a Clinical Update. Clin. Microbiol. Rev. 18: 657-686 [Abstract] [Full Text]  
  • Schlesinger, J., Navon-Venezia, S., Chmelnitsky, I., Hammer-Munz, O., Leavitt, A., Gold, H. S., Schwaber, M. J., Carmeli, Y. (2005). Extended-Spectrum Beta-Lactamases among Enterobacter Isolates Obtained in Tel Aviv, Israel. Antimicrob. Agents Chemother. 49: 1150-1156 [Abstract] [Full Text]  
  • Jacoby, G. A., Munoz-Price, L. S. (2005). The New {beta}-Lactamases. NEJM 352: 380-391 [Full Text]  
  • Wachino, J.-i., Doi, Y., Yamane, K., Shibata, N., Yagi, T., Kubota, T., Ito, H., Arakawa, Y. (2004). Nosocomial Spread of Ceftazidime-Resistant Klebsiella pneumoniae Strains Producing a Novel Class A {beta}-Lactamase, GES-3, in a Neonatal Intensive Care Unit in Japan. Antimicrob. Agents Chemother. 48: 1960-1967 [Abstract] [Full Text]  
  • Henriques, I., Moura, A., Alves, A., Saavedra, M. J., Correia, A. (2004). Molecular Characterization of a Carbapenem-Hydrolyzing Class A {beta}-Lactamase, SFC-1, from Serratia fonticola UTAD54. Antimicrob. Agents Chemother. 48: 2321-2324 [Abstract] [Full Text]  
  • Bonnet, R. (2004). Growing Group of Extended-Spectrum {beta}-Lactamases: the CTX-M Enzymes. Antimicrob. Agents Chemother. 48: 1-14 [Full Text]  
  • Bell, J. M., Turnidge, J. D., Jones, R. N., the SENTRY Asia-Pacific Participants,, (2003). Prevalence of Extended-Spectrum {beta}-Lactamase-Producing Enterobacter cloacae in the Asia-Pacific Region: Results from the SENTRY Antimicrobial Surveillance Program, 1998 to 2001. Antimicrob. Agents Chemother. 47: 3989-3993 [Abstract] [Full Text]  
  • Saavedra, M. J., Peixe, L., Sousa, J. C., Henriques, I., Alves, A., Correia, A. (2003). Sfh-I, a Subclass B2 Metallo-{beta}-Lactamase from a Serratia fonticola Environmental Isolate. Antimicrob. Agents Chemother. 47: 2330-2333 [Abstract] [Full Text]  
  • Vimont, S., Poirel, L., Naas, T., Nordmann, P. (2002). Identification of a Chromosome-Borne Expanded-Spectrum Class A {beta}-Lactamase from Erwinia persicina. Antimicrob. Agents Chemother. 46: 3401-3405 [Abstract] [Full Text]  
  • Morin, A.-S., Poirel, L., Mory, F., Labia, R., Nordmann, P. (2002). Biochemical-Genetic Analysis and Distribution of DES-1, an Ambler Class A Extended-Spectrum {beta}-Lactamase from Desulfovibrio desulfuricans. Antimicrob. Agents Chemother. 46: 3215-3222 [Abstract] [Full Text]  
  • Humeniuk, C., Arlet, G., Gautier, V., Grimont, P., Labia, R., Philippon, A. (2002). {beta}-Lactamases of Kluyvera ascorbata, Probable Progenitors of Some Plasmid-Encoded CTX-M Types. Antimicrob. Agents Chemother. 46: 3045-3049 [Abstract] [Full Text]  
  • Arpin, C., Labia, R., Dubois, V., Noury, P., Souquet, M., Quentin, C. (2002). TEM-80, a Novel Inhibitor-Resistant {beta}-Lactamase in a Clinical Isolate of Enterobacter cloacae. Antimicrob. Agents Chemother. 46: 1183-1189 [Abstract] [Full Text]  
  • Decousser, J. W., Poirel, L., Nordmann, P. (2001). Characterization of a Chromosomally Encoded Extended-Spectrum Class A beta -Lactamase from Kluyvera cryocrescens. Antimicrob. Agents Chemother. 45: 3595-3598 [Abstract] [Full Text]  
  • Bradford, P. A. (2001). Extended-Spectrum {beta}-Lactamases in the 21st Century: Characterization, Epidemiology, and Detection of This Important Resistance Threat. Clin. Microbiol. Rev. 14: 933-951 [Abstract] [Full Text]  
  • Naas, T., Massuard, S., Garnier, F., Nordmann, P. (2001). AmpD Is Required for Regulation of Expression of NmcA, a Carbapenem-Hydrolyzing {beta}-Lactamase of Enterobacter cloacae. Antimicrob. Agents Chemother. 45: 2908-2915 [Abstract] [Full Text]  
  • Bellais, S., Poirel, L., Fortineau, N., Decousser, J. W., Nordmann, P. (2001). Biochemical-Genetic Characterization of the Chromosomally Encoded Extended-Spectrum Class A {beta}-Lactamase from Rahnella aquatilis. Antimicrob. Agents Chemother. 45: 2965-2968 [Abstract] [Full Text]  
  • Bonnet, R., Dutour, C., Sampaio, J. L. M., Chanal, C., Sirot, D., Labia, R., De Champs, C., Sirot, J. (2001). Novel Cefotaximase (CTX-M-16) with Increased Catalytic Efficiency Due to Substitution Asp-240{right-arrow}Gly. Antimicrob. Agents Chemother. 45: 2269-2275 [Abstract] [Full Text]  
  • Bonnet, R., Sampaio, J. L. M., Chanal, C., Sirot, D., De Champs, C., Viallard, J. L., Labia, R., Sirot, J. (2000). A Novel Class A Extended-Spectrum beta -Lactamase (BES-1) in Serratia marcescens Isolated in Brazil. Antimicrob. Agents Chemother. 44: 3061-3068 [Abstract] [Full Text]  
  • Giakkoupi, P., Tzouvelekis, L. S., Tsakris, A., Loukova, V., Sofianou, D., Tzelepi, E. (2000). IBC-1, a Novel Integron-Associated Class A beta -Lactamase with Extended-Spectrum Properties Produced by an Enterobacter cloacae Clinical Strain. Antimicrob. Agents Chemother. 44: 2247-2253 [Abstract] [Full Text]  
  • Bonnet, R., Sampaio, J. L. M., Labia, R., De Champs, C., Sirot, D., Chanal, C., Sirot, J. (2000). A Novel CTX-M beta -Lactamase (CTX-M-8) in Cefotaxime-Resistant Enterobacteriaceae Isolated in Brazil. Antimicrob. Agents Chemother. 44: 1936-1942 [Abstract] [Full Text]  
  • Poirel, L., Le Thomas, I., Naas, T., Karim, A., Nordmann, P. (2000). Biochemical Sequence Analyses of GES-1, a Novel Class A Extended-Spectrum beta -Lactamase, and the Class 1 Integron In52 from Klebsiella pneumoniae. Antimicrob. Agents Chemother. 44: 622-632 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»