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Antimicrobial Agents and Chemotherapy, February 1999, p. 354-356, Vol. 43, No. 2
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Efflux Pump-Mediated Quinolone Resistance in Staphylococcus aureus Strains Wild Type for gyrA, gyrB, grlA, and norA

Juan Luis Muñoz-Bellido,1 M. A. Alonso Manzanares,1 J. A. Martínez Andrés,2 M. N. Gutiérrez Zufiaurre,1 G. Ortiz,2 M. Segovia Hernández,2 and J. A. García-Rodríguez1,*

Departamento de Microbiologia, Hospital Universitario de Salamanca, Salamanca,1 and Departamento de Microbiologia, Facultad de Medicina, Hospital General Universitario, Murcia,2 Spain

Received 29 May 1998/Returned for modification 16 July 1998/Accepted 12 November 1998

Fluoroquinolone efflux was studied in 47 Staphylococcus aureus clinical strains with MICs of ciprofloxacin (CFX) of <= 2 µg/ml. Forty-three strains were wild type for gyrA, gyrB, and grlA quinolone resistance-determining regions and for norA and its promoter region. Forty of these strains (MICs of CFX, 0.1 to 0.2 µg/ml) did not show efflux of fluoroquinolones. Three strains (MICs of CFX, 1 to 2 µg/ml) showed efflux. These results suggest that efflux can appear in S. aureus clinical strains in the absence of mutations in norA and its promoter.

* Corresponding author. Mailing address: Departamento de Microbiología, Hospital Universitario de Salamanca, Paseo de San Vicente 108, 37007 Salamanca, Spain. Phone: 34-923-264825. Fax: 34-923-262261. E-mail: jagarrod{at}gugu.usal.es.

Antimicrobial Agents and Chemotherapy, February 1999, p. 354-356, Vol. 43, No. 2
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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