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Antimicrobial Agents and Chemotherapy, February 1999, p. 400-402, Vol. 43, No. 2
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Interplay between Chromosomal beta -Lactamase and the MexAB-OprM Efflux System in Intrinsic Resistance to beta -Lactams in Pseudomonas aeruginosa

Nobuhisa Masuda,1,* Naomasa Gotoh,2 Chie Ishii,1 Eiko Sakagawa,1 Satoshi Ohya,1 and Takeshi Nishino2

Biological Research Laboratories, Sankyo Co., Ltd., Shinagawa-ku, Tokyo 140-8710,1 and Department of Microbiology, Kyoto Pharmaceutical University, Yamashina, Kyoto 607-8414,2 Japan

Received 29 July 1998/Returned for modification 10 September 1998/Accepted 9 November 1998

We investigated the role of chromosomal beta -lactamase and the MexAB-OprM efflux system in intrinsic resistance to beta -lactams in Pseudomonas aeruginosa. Determination of the susceptibilities of a series of isogenic mutants with impaired production of the beta -lactamase and the efflux system to 16 beta -lactams including penicillins, cephems, oxacephems, carbapenems, and a monobactam demonstrated that the intrinsic resistance of P. aeruginosa to most of the beta -lactams is due to the interplay of both factors.

* Corresponding author. Mailing address: Biological Research Laboratories, Sankyo Co., Ltd., 2-58 Hiromachi 1-chome, Shinagawa-ku, Tokyo 140-8710, Japan. Phone: 81-3-3492-3131. Fax: 81-3-5436-8566. E-mail: nmasud{at}shina.sankyo.co.jp.

Antimicrobial Agents and Chemotherapy, February 1999, p. 400-402, Vol. 43, No. 2
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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