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Antimicrobial Agents and Chemotherapy, February 1999, p. 424-427, Vol. 43, No. 2
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Carbapenem Activities against Pseudomonas aeruginosa: Respective Contributions of OprD and Efflux Systems

Thilo Köhler,* Mehri Michea-Hamzehpour, Simone F. Epp, and Jean-Claude Pechere

Department of Genetics and Microbiology, Centre Médical Universitaire, CH-1211 Geneva 4, Switzerland

Received 12 June 1998/Returned for modification 2 October 1998/Accepted 24 November 1998

While meropenem MICs were strongly influenced by the presence or absence of the MexAB-OprM efflux pump in both OprD-proficient and -deficient strain backgrounds, MICs of imipenem and of ER-35786 remained unchanged, demonstrating that meropenem is a substrate of MexAB-OprM but not imipenem and ER-35786. In vitro, all three carbapenems selected loss of OprD as a first mechanism of resistance. However, in an OprD-deficient background, meropenem was able to select MexAB-OprM overproducers as a secondary resistance mechanism, while ER-35786 selected a mutant cross-resistant to sparfloxacin and cefpirome.

* Corresponding author. Mailing address: Department of Genetics and Microbiology, CMU, 9, av. de Champel, CH-1211 Geneva 4, Switzerland. Phone: 41-22-7025655. Fax: 41-22-7025702. E-mail: Thilo.Kohler{at}medecine.unige.ch.

Antimicrobial Agents and Chemotherapy, February 1999, p. 424-427, Vol. 43, No. 2
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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