This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Willems, R. J. L.
Right arrow Articles by van Embden, J. D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Willems, R. J. L.
Right arrow Articles by van Embden, J. D. A.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, March 1999, p. 483-491, Vol. 43, No. 3
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Molecular Diversity and Evolutionary Relationships of Tn1546-Like Elements in Enterococci from Humans and Animals

Rob J. L. Willems,1,* Janetta Top,1 Nicole van den Braak,2 Alex van Belkum,2 Dik J. Mevius,3 Giel Hendriks,1 Marga van Santen-Verheuvel,1 and Jan D. A. van Embden1

Research Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, 3720 BA Bilthoven,1 Department of Medical Microbiology & Infectious Diseases, Erasmus Medical Center Rotterdam, 3015 GD Rotterdam,2 and Department of Bacteriology, DLO-Institute for Animal Science and Health, 8200 AB Lelystad,3 The Netherlands

Received 20 July 1998/Returned for modification 5 October 1998/Accepted 14 December 1998

We report on a detailed study on the molecular diversity and evolutionary relationships of Tn1546-like elements in vancomycin-resistant enterococci (VRE) from humans and animals. Restriction fragment length polymorphism (RFLP) analysis of the VanA transposon of 97 VRE revealed seven different Tn1546 types. Subsequent sequencing of the complete VanA transposons of 13 VRE isolates representing the seven RFLP types followed by sequencing of the identified polymorphic regions in 84 other VanA transposons resulted in the identification of 22 different Tn1546 derivatives. Differences between the Tn1546 types included point mutations in orf1, vanS, vanA, vanX, and vanY. Moreover, insertions of an IS1216V-IS3-like element in orf1, of IS1251 in the vanS-vanH intergenic region, and of IS1216V in the vanX-vanY intergenic region were found. The presence of insertion sequence elements was often associated with deletions in Tn1546. Identical Tn1546 types were found among isolates from humans and farm animals in The Netherlands, suggesting the sharing of a common vancomycin resistance gene pool. Application of the genetic analysis of Tn1546 to VRE isolates causing infections in hospitals in Oxford, United Kingdom, and Chicago, Ill., suggested the possibility of the horizontal transmission of the vancomycin resistance transposon. The genetic diversity in Tn1546 combined with epidemiological data suggest that the DNA polymorphism among Tn1546 variants can successfully be exploited for the tracing of the routes of transmission of vancomycin resistance genes.

* Corresponding author. Mailing address: Research Laboratory for Infectious Diseases (LIO), National Institute of Public Health and the Environment, P.O. Box 1, 3720 BA Bilthoven, The Netherlands. Phone: 31.30.2744050. Fax: 31.30.2744449. E-mail: rob.willems{at}rivm.nl.

Antimicrobial Agents and Chemotherapy, March 1999, p. 483-491, Vol. 43, No. 3
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Bellanger, X., Roberts, A. P., Morel, C., Choulet, F., Pavlovic, G., Mullany, P., Decaris, B., Guedon, G. (2009). Conjugative Transfer of the Integrative Conjugative Elements ICESt1 and ICESt3 from Streptococcus thermophilus. J. Bacteriol. 191: 2764-2775 [Abstract] [Full Text]  
  • Garcia-Migura, L., Hasman, H., Svendsen, C., Jensen, L. B. (2008). Relevance of hot spots in the evolution and transmission of Tn1546 in glycopeptide-resistant Enterococcus faecium (GREF) from broiler origin. J Antimicrob Chemother 62: 681-687 [Abstract] [Full Text]  
  • Ergani-Ozcan, A., Naas, T., Baysan, B. O., Ogunc, D., Inan, D., Colak, D., Nordmann, P. (2008). Nosocomial outbreak of vancomycin-resistant Enterococcus faecium in a paediatric unit at a Turkish university hospital. J Antimicrob Chemother 61: 1033-1039 [Abstract] [Full Text]  
  • Naas, T., Cuzon, G., Villegas, M.-V., Lartigue, M.-F., Quinn, J. P., Nordmann, P. (2008). Genetic Structures at the Origin of Acquisition of the {beta}-Lactamase blaKPC Gene. Antimicrob. Agents Chemother. 52: 1257-1263 [Abstract] [Full Text]  
  • Novais, C., Freitas, A. R., Sousa, J. C., Baquero, F., Coque, T. M., Peixe, L. V. (2008). Diversity of Tn1546 and Its Role in the Dissemination of Vancomycin-Resistant Enterococci in Portugal. Antimicrob. Agents Chemother. 52: 1001-1008 [Abstract] [Full Text]  
  • Talebi, M., Pourshafie, M. R., Katouli, M., Mollby, R. (2008). Molecular Structure and Transferability of Tn1546-Like Elements in Enterococcus faecium Isolates from Clinical, Sewage, and Surface Water Samples in Iran. Appl. Environ. Microbiol. 74: 1350-1356 [Abstract] [Full Text]  
  • Park, I. J., Lee, W. G., Lim, Y. A., Cho, S. R. (2007). Genetic Rearrangements of Tn1546-Like Elements in Vancomycin-Resistant Enterococcus faecium Isolates Collected from Hospitalized Patients over a Seven-Year Period. J. Clin. Microbiol. 45: 3903-3908 [Abstract] [Full Text]  
  • Heikens, E., Bonten, M. J. M., Willems, R. J. L. (2007). Enterococcal Surface Protein Esp Is Important for Biofilm Formation of Enterococcus faecium E1162. J. Bacteriol. 189: 8233-8240 [Abstract] [Full Text]  
  • Zheng, B., Tomita, H., Xiao, Y. H., Wang, S., Li, Y., Ike, Y. (2007). Molecular Characterization of Vancomycin-Resistant Enterococcus faecium Isolates from Mainland China. J. Clin. Microbiol. 45: 2813-2818 [Abstract] [Full Text]  
  • Garcia-Migura, L., Liebana, E., Jensen, L. B. (2007). Transposon characterization of vancomycin-resistant Enterococcus faecium (VREF) and dissemination of resistance associated with transferable plasmids. J Antimicrob Chemother 60: 263-268 [Abstract] [Full Text]  
  • Lim, S.-K., Tanimoto, K., Tomita, H., Ike, Y. (2006). Pheromone-Responsive Conjugative Vancomycin Resistance Plasmids in Enterococcus faecalis Isolates from Humans and Chicken Feces.. Appl. Environ. Microbiol. 72: 6544-6553 [Abstract] [Full Text]  
  • Leavis, H. L., Willems, R. J. L., Top, J., Bonten, M. J. M. (2006). High-Level Ciprofloxacin Resistance from Point Mutations in gyrA and parC Confined to Global Hospital-Adapted Clonal Lineage CC17 of Enterococcus faecium.. J. Clin. Microbiol. 44: 1059-1064 [Abstract] [Full Text]  
  • Lester, C. H., Frimodt-Moller, N., Sorensen, T. L., Monnet, D. L., Hammerum, A. M. (2006). In Vivo Transfer of the vanA Resistance Gene from an Enterococcus faecium Isolate of Animal Origin to an E. faecium Isolate of Human Origin in the Intestines of Human Volunteers. Antimicrob. Agents Chemother. 50: 596-599 [Abstract] [Full Text]  
  • Tsai, J.-C., Hsueh, P.-R., Chen, H.-J., Tseng, S.-P., Chen, P.-Y., Teng, L.-J. (2005). The erm(T) Gene Is Flanked by IS1216V in Inducible Erythromycin-Resistant Streptococcus gallolyticus subsp. pasteurianus. Antimicrob. Agents Chemother. 49: 4347-4350 [Abstract] [Full Text]  
  • Kuhn, I., Iversen, A., Finn, M., Greko, C., Burman, L. G., Blanch, A. R., Vilanova, X., Manero, A., Taylor, H., Caplin, J., Dominguez, L., Herrero, I. A., Moreno, M. A., Mollby, R. (2005). Occurrence and Relatedness of Vancomycin-Resistant Enterococci in Animals, Humans, and the Environment in Different European Regions. Appl. Environ. Microbiol. 71: 5383-5390 [Abstract] [Full Text]  
  • Naas, T., Fortineau, N., Snanoudj, R., Spicq, C., Durrbach, A., Nordmann, P. (2005). First Nosocomial Outbreak of Vancomycin-Resistant Enterococcus faecium Expressing a VanD-Like Phenotype Associated with a vanA Genotype. J. Clin. Microbiol. 43: 3642-3649 [Abstract] [Full Text]  
  • Coque, T. M., Willems, R. J. L., Fortun, J., Top, J., Diz, S., Loza, E., Canton, R., Baquero, F. (2005). Population Structure of Enterococcus faecium Causing Bacteremia in a Spanish University Hospital: Setting the Scene for a Future Increase in Vancomycin Resistance?. Antimicrob. Agents Chemother. 49: 2693-2700 [Abstract] [Full Text]  
  • Novais, C., Coque, T. M., Ferreira, H., Sousa, J. C., Peixe, L. (2005). Environmental Contamination with Vancomycin-Resistant Enterococci from Hospital Sewage in Portugal. Appl. Environ. Microbiol. 71: 3364-3368 [Abstract] [Full Text]  
  • De Leener, E., Martel, A., De Graef, E. M., Top, J., Butaye, P., Haesebrouck, F., Willems, R., Decostere, A. (2005). Molecular Analysis of Human, Porcine, and Poultry Enterococcus faecium Isolates and Their erm(B) Genes. Appl. Environ. Microbiol. 71: 2766-2770 [Abstract] [Full Text]  
  • Shaaly, A., Tellevik, M. G., Langeland, N., Hoiby, E A., Jureen, R. (2005). Comparison of serotyping, pulsed field gel electrophoresis and amplified fragment length polymorphism for typing of Streptococcus pneumoniae. J Med Microbiol 54: 467-472 [Abstract] [Full Text]  
  • Jung, W. K., Hong, S. K., Koo, H. C., Kwon, N. H., Park, Y. H. (2005). Nucleotide Sequence of IS1678, an Insertion Sequence in the vanA Cluster of Enterococci. Antimicrob. Agents Chemother. 49: 1666-1667 [Full Text]  
  • Clark, N. C., Weigel, L. M., Patel, J. B., Tenover, F. C. (2005). Comparison of Tn1546-Like Elements in Vancomycin-Resistant Staphylococcus aureus Isolates from Michigan and Pennsylvania. Antimicrob. Agents Chemother. 49: 470-472 [Abstract] [Full Text]  
  • Johnsen, P. J., Osterhus, J. I., Sletvold, H., Sorum, M., Kruse, H., Nielsen, K., Simonsen, G. S., Sundsfjord, A. (2005). Persistence of Animal and Human Glycopeptide-Resistant Enterococci on Two Norwegian Poultry Farms Formerly Exposed to Avoparcin Is Associated with a Widespread Plasmid-Mediated vanA Element within a Polyclonal Enterococcus faecium Population. Appl. Environ. Microbiol. 71: 159-168 [Abstract] [Full Text]  
  • Top, J., Schouls, L. M., Bonten, M. J. M., Willems, R. J. L. (2004). Multiple-Locus Variable-Number Tandem Repeat Analysis, a Novel Typing Scheme To Study the Genetic Relatedness and Epidemiology of Enterococcus faecium Isolates. J. Clin. Microbiol. 42: 4503-4511 [Abstract] [Full Text]  
  • Novais, C., Coque, T. M., Sousa, J. C., Baquero, F., Peixe, L. (2004). Local Genetic Patterns within a Vancomycin-Resistant Enterococcus faecalis Clone Isolated in Three Hospitals in Portugal. Antimicrob. Agents Chemother. 48: 3613-3617 [Abstract] [Full Text]  
  • Lovseth, A., Loncarevic, S., Berdal, K. G. (2004). Modified Multiplex PCR Method for Detection of Pyrogenic Exotoxin Genes in Staphylococcal Isolates. J. Clin. Microbiol. 42: 3869-3872 [Abstract] [Full Text]  
  • Huh, J. Y., Lee, W. G., Lee, K., Shin, W. S., Yoo, J. H. (2004). Distribution of Insertion Sequences Associated with Tn1546-Like Elements among Enterococcus faecium Isolates from Patients in Korea. J. Clin. Microbiol. 42: 1897-1902 [Abstract] [Full Text]  
  • Mohn, S. C., Ulvik, A., Jureen, R., Willems, R. J. L., Top, J., Leavis, H., Harthug, S., Langeland, N. (2004). Duplex Real-Time PCR Assay for Rapid Detection of Ampicillin-Resistant Enterococcus faecium. Antimicrob. Agents Chemother. 48: 556-560 [Abstract] [Full Text]  
  • Guardabassi, L., Dalsgaard, A. (2004). Occurrence, Structure, and Mobility of Tn1546-Like Elements in Environmental Isolates of Vancomycin-Resistant Enterococci. Appl. Environ. Microbiol. 70: 984-990 [Abstract] [Full Text]  
  • Leavis, H., Top, J., Shankar, N., Borgen, K., Bonten, M., van Embden, J., Willems, R. J. L. (2004). A Novel Putative Enterococcal Pathogenicity Island Linked to the esp Virulence Gene of Enterococcus faecium and Associated with Epidemicity. J. Bacteriol. 186: 672-682 [Abstract] [Full Text]  
  • Phillips, I., Casewell, M., Cox, T., De Groot, B., Friis, C., Jones, R., Nightingale, C., Preston, R., Waddell, J. (2004). Does the use of antibiotics in food animals pose a risk to human health? A critical review of published data. J Antimicrob Chemother 53: 28-52 [Abstract] [Full Text]  
  • Schouls, L. M., Schot, C. S., Jacobs, J. A. (2003). Horizontal Transfer of Segments of the 16S rRNA Genes between Species of the Streptococcus anginosus Group. J. Bacteriol. 185: 7241-7246 [Abstract] [Full Text]  
  • Foglia, G., Del Grosso, M., Vignaroli, C., Bagnarelli, P., Varaldo, P. E., Pantosti, A., Biavasco, F. (2003). Molecular analysis of Tn1546-like elements mediating high-level vancomycin resistance in Enterococcus gallinarum. J Antimicrob Chemother 52: 772-775 [Abstract] [Full Text]  
  • Jureen, R., Top, J., Mohn, S. C., Harthug, S., Langeland, N., Willems, R. J. L. (2003). Molecular Characterization of Ampicillin-Resistant Enterococcus faecium Isolates from Hospitalized Patients in Norway. J. Clin. Microbiol. 41: 2330-2336 [Abstract] [Full Text]  
  • Manson, J. M., Keis, S., Smith, J. M. B., Cook, G. M. (2003). A Clonal Lineage of VanA-Type Enterococcus faecalis Predominates in Vancomycin-Resistant Enterococci Isolated in New Zealand. Antimicrob. Agents Chemother. 47: 204-210 [Abstract] [Full Text]  
  • Simjee, S., White, D. G., McDermott, P. F., Wagner, D. D., Zervos, M. J., Donabedian, S. M., English, L. L., Hayes, J. R., Walker, R. D. (2002). Characterization of Tn1546 in Vancomycin-Resistant Enterococcus faecium Isolated from Canine Urinary Tract Infections: Evidence of Gene Exchange between Human and Animal Enterococci. J. Clin. Microbiol. 40: 4659-4665 [Abstract] [Full Text]  
  • Kholodii, G., Gorlenko, Zh., Mindlin, S., Hobman, J., Nikiforov, V. (2002). Tn5041-like transposons: molecular diversity, evolutionary relationships and distribution of distinct variants in environmental bacteria. Microbiology 148: 3569-3582 [Abstract] [Full Text]  
  • Bruinsma, N., Willems, R. J. L., van den Bogaard, A. E., van Santen-Verheuvel, M., London, N., Driessen, C., Stobberingh, E. E. (2002). Different Levels of Genetic Homogeneity in Vancomycin-Resistant and -Susceptible Enterococcus faecium Isolates from Different Human and Animal Sources Analyzed by Amplified-Fragment Length Polymorphism. Antimicrob. Agents Chemother. 46: 2779-2783 [Abstract] [Full Text]  
  • van den Bogaard, A. E., Willems, R., London, N., Top, J., Stobberingh, E. E. (2002). Antibiotic resistance of faecal enterococci in poultry, poultry farmers and poultry slaughterers. J Antimicrob Chemother 49: 497-505 [Abstract] [Full Text]  
  • DEMERTZI, E., PALEPOU, M-F.I., KAUFMANN, M.E., AVLAMIS, A., WOODFORD, N. (2001). Characterisation of VanA and VanB elements from glycopeptide-resistant Enterococcus faecium from Greece. J Med Microbiol 50: 682-687 [Abstract] [Full Text]  
  • van Belkum, A., Struelens, M., de Visser, A., Verbrugh, H., Tibayrenc, M. (2001). Role of Genomic Typing in Taxonomy, Evolutionary Genetics, and Microbial Epidemiology. Clin. Microbiol. Rev. 14: 547-560 [Abstract] [Full Text]  
  • Brown, A. R., Townsley, A. C., Amyes, S. G. B. (2001). Diversity of Tn1546 Elements in Clinical Isolates of Glycopeptide-Resistant Enterococci from Scottish Hospitals. Antimicrob. Agents Chemother. 45: 1309-1311 [Abstract] [Full Text]  
  • Schouten, M. A., Willems, R. J. L., Kraak, W. A. G., Top, J., Hoogkamp-Korstanje, J. A. A., Voss, A. (2001). Molecular Analysis of Tn1546-Like Elements in Vancomycin-Resistant Enterococci Isolated from Patients in Europe Shows Geographic Transposon Type Clustering. Antimicrob. Agents Chemother. 45: 986-989 [Abstract] [Full Text]  
  • Kariyama, R., Kumon, H., Hammerum, A. M., Aarestrup, F. M., Jensen, L. B. (2001). Identification of a Tn1546-Like (Type 2) Element in Vancomycin-Resistant Enterococcus faecium Isolated from Hospitalized Patients in Japan. Antimicrob. Agents Chemother. 45: 992-993 [Full Text]  
  • Dalla Costa, L. M., Reynolds, P. E., Souza, H. A. P. H. M., Souza, D. C., Palepou, M.-F. I., Woodford, N. (2000). Characterization of a Divergent vanD-Type Resistance Element from the First Glycopeptide-Resistant Strain of Enterococcus faecium Isolated in Brazil. Antimicrob. Agents Chemother. 44: 3444-3446 [Abstract] [Full Text]  
  • McGregor, K. F., Young, H.-K. (2000). Identification and Characterization of vanB2 Glycopeptide Resistance Elements in Enterococci Isolated in Scotland. Antimicrob. Agents Chemother. 44: 2341-2348 [Abstract] [Full Text]  
  • Robredo, B., Torres, C., Singh, K. V., Murray, B. E. (2000). Molecular Analysis of Tn1546 in vanA-Containing Enterococcus spp. Isolated from Humans and Poultry. Antimicrob. Agents Chemother. 44: 2588-2589 [Full Text]  
  • Donabedian, S., Hershberger, E., Thal, L. A., Chow, J. W., Clewell, D. B., Robinson-Dunn, B., Zervos, M. J. (2000). PCR Fragment Length Polymorphism Analysis of Vancomycin-Resistant Enterococcus faecium. J. Clin. Microbiol. 38: 2885-2888 [Abstract] [Full Text]  
  • Aarestrup, F. M. (2000). Characterization of Glycopeptide-Resistant Enterococcus faecium (GRE) from Broilers and Pigs in Denmark: Genetic Evidence that Persistence of GRE in Pig Herds Is Associated with Coselection by Resistance to Macrolides. J. Clin. Microbiol. 38: 2774-2777 [Abstract] [Full Text]  
  • Darini, A. L. C., Palepou, M.-F. I., Woodford, N. (2000). Effects of the Movement of Insertion Sequences on the Structure of VanA Glycopeptide Resistance Elements in Enterococcus faecium. Antimicrob. Agents Chemother. 44: 1362-1364 [Abstract] [Full Text]  
  • Stobberingh, E., van den Bogaard, A., London, N., Driessen, C., Top, J., Willems, R. (1999). Enterococci with Glycopeptide Resistance in Turkeys, Turkey Farmers, Turkey Slaughterers, and (Sub)Urban Residents in the South of The Netherlands: Evidence for Transmission of Vancomycin Resistance from Animals to Humans?. Antimicrob. Agents Chemother. 43: 2215-2221 [Abstract] [Full Text]  
  • Descheemaeker, P. R. M., Chapelle, S., Devriese, L. A., Butaye, P., Vandamme, P., Goossens, H. (1999). Comparison of Glycopeptide-Resistant Enterococcus faecium Isolates and Glycopeptide Resistance Genes of Human and Animal Origins. Antimicrob. Agents Chemother. 43: 2032-2037 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»