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Antimicrobial Agents and Chemotherapy, March 1999, p. 683-685, Vol. 43, No. 3
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Multiplex Sequence Analysis Demonstrates the Competitive Growth Advantage of the A-to-G Mutants of Clarithromycin-Resistant Helicobacter pylori

Ge Wang,1 M. Sayeedur Rahman,2 M. Zafri Humayun,2 and Diane E. Taylor1,*

Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada,1 and Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry New Jersey Medical School, Newark, New Jersey2

Received 10 August 1998/Returned for modification 23 November 1998/Accepted 13 December 1998

Clarithromycin resistance in Helicobacter pylori is due to point mutation within the 23S rRNA. We examined the growth rates of different types of site-directed mutants and demonstrated quantitatively the competitive growth advantage of A-to-G mutants over other types of mutants by a multiplex sequencing assay. The results provide a rational explanation of why A-to-G mutants are predominantly observed among clarithromycin-resistant clinical isolates.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, AB, Canada T6G 2H7. Phone: (780) 492-4777. Fax: (780) 492-7521. E-mail: diane.taylor{at}ualberta.ca.

Antimicrobial Agents and Chemotherapy, March 1999, p. 683-685, Vol. 43, No. 3
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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