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Antimicrobial Agents and Chemotherapy, March 1999, p. 686-689, Vol. 43, No. 3
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Many Class I Integrons Comprise Distinct Stable Structures Occurring in Different Species of Enterobacteriaceae Isolated from Widespread Geographic Regions in Europe

Pedro Martinez-Freijo, Ad C. Fluit, Franz-Joseph Schmitz, Jan Verhoef, and Mark E. Jones*

Eijkman-Winkler Institute for Clinical Microbiology, University Hospital of Utrecht, 3584CX Utrecht, The Netherlands

Received 22 June 1998/Returned for modification 15 October 1998/Accepted 22 December 1998

Three sizes of inserted regions of DNA (800, 1,000, and 1,500 bp) were shown to be common among class I integrons in unrelated clinical isolates of Enterobacteriaceae from different European hospitals. Sequencing showed that 800-bp inserted regions comprised identical sequences including aacA4, that 1,000-bp inserted regions included aadA, and that 1,500-bp inserted regions included dfrI and aadA1, irrespective of host species and geographic origin. In addition promoter sequences were mostly identical for each size class. These data suggest that inserted gene cassettes and promoter regions of integrons are conserved and stable, with resistance genes transferred more often as part of the entire integron structure than as individual gene cassettes.

* Corresponding author. Present address: MRL Pharmaceutical Services, Den Brielstraat 11, 3554XD Utrecht, The Netherlands. Phone: 31 30 265 1794. Fax: 31 30 265 1784. E-mail: mjones{at}thetsn.com.

Antimicrobial Agents and Chemotherapy, March 1999, p. 686-689, Vol. 43, No. 3
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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