This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Falbo, V.
Right arrow Articles by Luzzi, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Falbo, V.
Right arrow Articles by Luzzi, I.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, March 1999, p. 693-696, Vol. 43, No. 3
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Antibiotic Resistance Conferred by a Conjugative Plasmid and a Class I Integron in Vibrio cholerae O1 El Tor Strains Isolated in Albania and Italy

Vincenzo Falbo,1,* Alessandra Carattoli,2 Fabio Tosini,3 Cristina Pezzella,2 Anna Maria Dionisi,2 and Ida Luzzi2

Laboratories of Ultrastructures,1 Bacteriology and Medical Mycology,2 and Cellular Biology,3 Istituto Superiore di Sanità, 00161 Rome, Italy

Received 9 September 1998/Returned for modification 21 October 1998/Accepted 30 December 1998

Multidrug-resistant Vibrio cholerae O1 El Tor strains isolated during the 1994 outbreak of cholera in Albania and Italy were characterized for the molecular basis of antibiotic resistance. All strains were found to be resistant to tetracycline, streptomycin, spectinomycin, trimethoprim, sulfathiazole, and the vibriostatic compound O/129 (2,4-diamino-6,7-diisopropylteridine). Resistance genes were self-transferable by a conjugative plasmid of about 60 MDa, with the exception of spectinomycin resistance, which was conferred by the aadA1 gene cassette located in the bacterial chromosome within a class 1 integron. The resistance to trimethoprim and O/129 was conferred by the dfrA1 gene, which was present on the plasmid. Although the dfrA1 gene is known to be borne on an integron cassette, class 1, 2, or 3 intI genes were not detected as part of the plasmid DNA from the strains studied.

* Corresponding author. Mailing address: Laboratory of Ultrastructures, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161 Rome, Italy. Phone: 39 6 4990 2805. Fax: 39 6 49387112.

Antimicrobial Agents and Chemotherapy, March 1999, p. 693-696, Vol. 43, No. 3
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Pan, J.-C., Ye, R., Wang, H.-Q., Xiang, H.-Q., Zhang, W., Yu, X.-F., Meng, D.-M., He, Z.-S. (2008). Vibrio cholerae O139 Multiple-Drug Resistance Mediated by Yersinia pestis pIP1202-Like Conjugative Plasmids. Antimicrob. Agents Chemother. 52: 3829-3836 [Abstract] [Full Text]  
  • Ramachandran, D., Bhanumathi, R., Singh, D. V. (2007). Multiplex PCR for detection of antibiotic resistance genes and the SXT element: application in the characterization of Vibrio cholerae. J Med Microbiol 56: 346-351 [Abstract] [Full Text]  
  • Ceccarelli, D., Salvia, A. M., Sami, J., Cappuccinelli, P., Colombo, M. M. (2006). New Cluster of Plasmid-Located Class 1 Integrons in Vibrio cholerae O1 and a dfrA15 Cassette-Containing Integron in Vibrio parahaemolyticus Isolated in Angola.. Antimicrob. Agents Chemother. 50: 2493-2499 [Abstract] [Full Text]  
  • Shi, L., Fujihara, K., Sato, T., Ito, H., Garg, P., Chakrabarty, R., Ramamurthy, T., Nair, G. B., Takeda, Y., Yamasaki, S. (2006). Distribution and characterization of integrons in various serogroups of Vibrio cholerae strains isolated from diarrhoeal patients between 1992 and 2000 in Kolkata, India.. J Med Microbiol 55: 575-583 [Abstract] [Full Text]  
  • Iwanaga, M., Toma, C., Miyazato, T., Insisiengmay, S., Nakasone, N., Ehara, M. (2004). Antibiotic Resistance Conferred by a Class I Integron and SXT Constin in Vibrio cholerae O1 Strains Isolated in Laos. Antimicrob. Agents Chemother. 48: 2364-2369 [Abstract] [Full Text]  
  • Vakulenko, S. B., Mobashery, S. (2003). Versatility of Aminoglycosides and Prospects for Their Future. Clin. Microbiol. Rev. 16: 430-450 [Abstract] [Full Text]  
  • Thungapathra, M., Amita, , Sinha, K. K., Chaudhuri, S. R., Garg, P., Ramamurthy, T., Nair, G. B., Ghosh, A. (2002). Occurrence of Antibiotic Resistance Gene Cassettes aac(6')-Ib, dfrA5, dfrA12, and ereA2 in Class I Integrons in Non-O1, Non-O139 Vibrio cholerae Strains in India. Antimicrob. Agents Chemother. 46: 2948-2955 [Abstract] [Full Text]  
  • Melano, R., Petroni, A., Garutti, A., Saka, H. A., Mange, L., Pasteran, F., Rapoport, M., Rossi, A., Galas, M. (2002). New Carbenicillin-Hydrolyzing {beta}-Lactamase (CARB-7) from Vibrio cholerae Non-O1, Non-O139 Strains Encoded by the VCR Region of the V. cholerae Genome. Antimicrob. Agents Chemother. 46: 2162-2168 [Abstract] [Full Text]  
  • Petroni, A., Corso, A., Melano, R., Cacace, M. L., Bru, A. M., Rossi, A., Galas, M. (2002). Plasmidic Extended-Spectrum {beta}-Lactamases in Vibrio cholerae O1 El Tor Isolates in Argentina. Antimicrob. Agents Chemother. 46: 1462-1468 [Abstract] [Full Text]  
  • Dalsgaard, A., Forslund, A., Sandvang, D., Arntzen, L., Keddy, K. (2001). Vibrio cholerae O1 outbreak isolates in Mozambique and South Africa in 1998 are multiple-drug resistant, contain the SXT element and the aadA2 gene located on class 1 integrons. J Antimicrob Chemother 48: 827-838 [Abstract] [Full Text]  
  • Hochhut, B., Lotfi, Y., Mazel, D., Faruque, S. M., Woodgate, R., Waldor, M. K. (2001). Molecular Analysis of Antibiotic Resistance Gene Clusters in Vibrio cholerae O139 and O1 SXT Constins. Antimicrob. Agents Chemother. 45: 2991-3000 [Abstract] [Full Text]  
  • Naas, T., Benaoudia, F., Massuard, S., Nordmann, P. (2000). Integron-located VEB-1 extended-spectrum {beta}-lactamase gene in a Proteus mirabilis clinical isolate from Vietnam. J Antimicrob Chemother 46: 703-711 [Abstract] [Full Text]  
  • Daly, M., Fanning, S. (2000). Characterization and Chromosomal Mapping of Antimicrobial Resistance Genes in Salmonella enterica Serotype Typhimurium. Appl. Environ. Microbiol. 66: 4842-4848 [Abstract] [Full Text]  
  • Dalsgaard, A., Forslund, A., Petersen, A., Brown, D. J., Dias, F., Monteiro, S., Molbak, K., Aaby, P., Rodrigues, A., Sandström, A. (2000). Class 1 Integron-Borne, Multiple-Antibiotic Resistance Encoded by a 150-Kilobase Conjugative Plasmid in Epidemic Vibrio cholerae O1 Strains Isolated in Guinea-Bissau. J. Clin. Microbiol. 38: 3774-3779 [Abstract] [Full Text]  
  • Clark, C. A., Purins, L., Kaewrakon, P., Focareta, T., Manning, P. A. (2000). The Vibrio cholerae O1 chromosomal integron. Microbiology 146: 2605-2612 [Abstract] [Full Text]  
  • Dalsgaard, A., Forslund, A., Serichantalergs, O., Sandvang, D. (2000). Distribution and Content of Class 1 Integrons in Different Vibrio cholerae O-Serotype Strains Isolated in Thailand. Antimicrob. Agents Chemother. 44: 1315-1321 [Abstract] [Full Text]  
  • Bass, L., Liebert, C. A., Lee, M. D., Summers, A. O., White, D. G., Thayer, S. G., Maurer, J. J. (1999). Incidence and Characterization of Integrons, Genetic Elements Mediating Multiple-Drug Resistance, in Avian Escherichia coli. Antimicrob. Agents Chemother. 43: 2925-2929 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»