This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bozdogan, B.
Right arrow Articles by Leclercq, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bozdogan, B.
Right arrow Articles by Leclercq, R.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, April 1999, p. 925-929, Vol. 43, No. 4
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A New Resistance Gene, linB, Conferring Resistance to Lincosamides by Nucleotidylation in Enterococcus faecium HM1025

Bülent Bozdogan,1,2 Latifa Berrezouga,1 Ming-Shang Kuo,3 David A. Yurek,3 Kathleen A. Farley,3 Brian J. Stockman,3 and Roland Leclercq1,2,*

Service de Bactériologie-Virologie, Hôpital Henri Mondor---Université Paris XII, 94000 Créteil,1 and Service de Microbiologie, Hôpital Côte de Nacre, Université de Caen, 14033 Caen,2 France, and Pharmacia and Upjohn, Kalamazoo, Michigan 490023

Received 3 August 1998/Returned for modification 15 September 1998/Accepted 6 January 1999

Resistance to lincomycin and clindamycin in the clinical isolate Enterococcus faecium HM1025 is due to a ribosomal methylase encoded by an ermAM-like gene and the plasmid-mediated inactivation of these antibiotics. We have cloned and determined the nucleotide sequence of the gene responsible for the inactivation of lincosamides, linB. This gene encodes a 267-amino-acid lincosamide nucleotidyltransferase. The enzyme catalyzes 3-(5'-adenylation) (the adenylation of the hydroxyl group in position 3 of the molecules) of lincomycin and clindamycin. Expression of linB was observed in both Escherichia coli and Staphylococcus aureus. The deduced amino acid sequence of the enzyme did not display any significant homology with staphylococcal nucleotidyltransferases encoded by linA and linA' genes. Sequences homologous to linB were found in 14 other clinical isolates of E. faecium, indicating the spread of the resistance trait in this species.

* Corresponding author. Mailing address: CHU de Caen, Service de Microbiologie, Avenue Côte de Nacre, 14033 Caen cedex, France. Phone: (33) 2 31 06 45 72. Fax: (33) 2 31 06 45 73. E-mail: leclercq-r{at}chu-caen.fr.

Antimicrobial Agents and Chemotherapy, April 1999, p. 925-929, Vol. 43, No. 4
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Rosander, A., Connolly, E., Roos, S. (2008). Removal of Antibiotic Resistance Gene-Carrying Plasmids from Lactobacillus reuteri ATCC 55730 and Characterization of the Resulting Daughter Strain, L. reuteri DSM 17938. Appl. Environ. Microbiol. 74: 6032-6040 [Abstract] [Full Text]  
  • Petinaki, E., Guerin-Faublee, V., Pichereau, V., Villers, C., Achard, A., Malbruny, B., Leclercq, R. (2008). Lincomycin Resistance Gene lnu(D) in Streptococcus uberis. Antimicrob. Agents Chemother. 52: 626-630 [Abstract] [Full Text]  
  • Schmitt-Van de Leemput, E., Zadoks, R. N. (2007). Genotypic and Phenotypic Detection of Macrolide and Lincosamide Resistance in Streptococcus uberis. J DAIRY SCI 90: 5089-5096 [Abstract] [Full Text]  
  • Luthje, P., von Kockritz-Blickwede, M., Schwarz, S. (2007). Identification and characterization of nine novel types of small staphylococcal plasmids carrying the lincosamide nucleotidyltransferase gene lnu(A). J Antimicrob Chemother 59: 600-606 [Abstract] [Full Text]  
  • Novotna, G., Janata, J. (2006). A New Evolutionary Variant of the Streptogramin A Resistance Protein, Vga(A)LC, from Staphylococcus haemolyticus with Shifted Substrate Specificity towards Lincosamides. Antimicrob. Agents Chemother. 50: 4070-4076 [Abstract] [Full Text]  
  • Levings, R. S., Hall, R. M., Lightfoot, D., Djordjevic, S. P. (2006). linG, a New Integron-Associated Gene Cassette Encoding a Lincosamide Nucleotidyltransferase.. Antimicrob. Agents Chemother. 50: 3514-3515 [Full Text]  
  • Gygax, S. E., Schuyler, J. A., Kimmel, L. E., Trama, J. P., Mordechai, E., Adelson, M. E. (2006). Erythromycin and clindamycin resistance in group B streptococcal clinical isolates.. Antimicrob. Agents Chemother. 50: 1875-1877 [Abstract] [Full Text]  
  • Zeng, X., Kong, F., Wang, H., Darbar, A., Gilbert, G. L. (2006). Simultaneous Detection of Nine Antibiotic Resistance-Related Genes in Streptococcus agalactiae Using Multiplex PCR and Reverse Line Blot Hybridization Assay. Antimicrob. Agents Chemother. 50: 204-209 [Abstract] [Full Text]  
  • Achard, A., Villers, C., Pichereau, V., Leclercq, R. (2005). New lnu(C) Gene Conferring Resistance to Lincomycin by Nucleotidylation in Streptococcus agalactiae UCN36. Antimicrob. Agents Chemother. 49: 2716-2719 [Abstract] [Full Text]  
  • von Both, U., Buerckstuemmer, A., Fluegge, K., Berner, R. (2005). Heterogeneity of Genotype-Phenotype Correlation among Macrolide-Resistant Streptococcus agalactiae Isolates. Antimicrob. Agents Chemother. 49: 3080-3082 [Abstract] [Full Text]  
  • Gonzalez, J. J., Andreu, A., the Spanish Group for the Study of Perinatal Infec, (2005). Multicenter Study of the Mechanisms of Resistance and Clonal Relationships of Streptococcus agalactiae Isolates Resistant to Macrolides, Lincosamides, and Ketolides in Spain. Antimicrob. Agents Chemother. 49: 2525-2527 [Abstract] [Full Text]  
  • Perreten, V., Vorlet-Fawer, L., Slickers, P., Ehricht, R., Kuhnert, P., Frey, J. (2005). Microarray-Based Detection of 90 Antibiotic Resistance Genes of Gram-Positive Bacteria. J. Clin. Microbiol. 43: 2291-2302 [Abstract] [Full Text]  
  • Duarte, R. S., Bellei, B. C., Miranda, O. P., Brito, M. A. V. P., Teixeira, L. M. (2005). Distribution of Antimicrobial Resistance and Virulence-Related Genes among Brazilian Group B Streptococci Recovered from Bovine and Human Sources. Antimicrob. Agents Chemother. 49: 97-103 [Abstract] [Full Text]  
  • Desjardins, M., Delgaty, K. L., Ramotar, K., Seetaram, C., Toye, B. (2004). Prevalence and Mechanisms of Erythromycin Resistance in Group A and Group B Streptococcus: Implications for Reporting Susceptibility Results. J. Clin. Microbiol. 42: 5620-5623 [Abstract] [Full Text]  
  • Malbruny, B., Werno, A. M., Anderson, T. P., Murdoch, D. R., Leclercq, R. (2004). A new phenotype of resistance to lincosamide and streptogramin A-type antibiotics in Streptococcus agalactiae in New Zealand. J Antimicrob Chemother 54: 1040-1044 [Abstract] [Full Text]  
  • Bozdogan, B., Galopin, S., Leclercq, R. (2004). Characterization of a New erm-Related Macrolide Resistance Gene Present in Probiotic Strains of Bacillus clausii. Appl. Environ. Microbiol. 70: 280-284 [Abstract] [Full Text]  
  • Bozdogan, B., Galopin, S., Gerbaud, G., Courvalin, P., Leclercq, R. (2003). Chromosomal aadD2 Encodes an Aminoglycoside Nucleotidyltransferase in Bacillus clausii. Antimicrob. Agents Chemother. 47: 1343-1346 [Abstract] [Full Text]  
  • de Azavedo, J. C. S., McGavin, M., Duncan, C., Low, D. E., McGeer, A. (2001). Prevalence and Mechanisms of Macrolide Resistance in Invasive and Noninvasive Group B Streptococcus Isolates from Ontario, Canada. Antimicrob. Agents Chemother. 45: 3504-3508 [Abstract] [Full Text]  
  • Fluit, A. C., Visser, M. R., Schmitz, F.-J. (2001). Molecular Detection of Antimicrobial Resistance. Clin. Microbiol. Rev. 14: 836-871 [Abstract] [Full Text]  
  • Fines, M., Leclercq, R. (2000). Activity of linezolid against Gram-positive cocci possessing genes conferring resistance to protein synthesis inhibitors. J Antimicrob Chemother 45: 797-802 [Abstract] [Full Text]  
  • Roberts, M. C., Sutcliffe, J., Courvalin, P., Jensen, L. B., Rood, J., Seppala, H. (1999). Nomenclature for Macrolide and Macrolide-Lincosamide-Streptogramin B Resistance Determinants. Antimicrob. Agents Chemother. 43: 2823-2830 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»