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Antimicrobial Agents and Chemotherapy, April 1999, p. 937-939, Vol. 43, No. 4
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Development of Resistance during Antimicrobial Therapy Caused by Insertion Sequence Interruption of Porin Genes

Santiago Hernández-Allés,1 Vicente J. Benedí,1 Luis Martínez-Martínez,2 Álvaro Pascual,2 Alicia Aguilar,3 Juan M. Tomás,3 and Sebastián Albertí1,*

<A><AC>A:</AC><AC>´</AC></A>rea de Microbiología, Departamento de Biología, Universidad de las Islas Baleares and IMEDEA (CSIC-UIB), Palma de Mallorca,1 Departamento de Microbiología, Universidad de Sevilla, Seville,2 and Departamento de Microbiología, Universidad de Barcelona, Barcelona,3 Spain

Received 10 August 1998/Returned for modification 23 November 1998/Accepted 22 January 1999

We have demonstrated by using an in vitro approach that interruption of the OmpK36 porin gene by insertion sequences (ISs) is a common type of mutation that causes loss of porin expression and increased resistance to cefoxitin in Klebsiella pneumoniae. This mechanism also operates in vivo: of 13 porin-deficient cefoxitin-resistant clinical isolates of K. pneumoniae, 4 presented ISs in their ompK36 gene.

* Corresponding author. Mailing address: UIB-Microbiología, Carretera de Valldemosa Km. 7.5, 07071-Palma de Mallorca, Spain. Phone: 34-971-173335. Fax: 34-971-173184. E-mail: dbasas3{at}clust.uib.es.

Antimicrobial Agents and Chemotherapy, April 1999, p. 937-939, Vol. 43, No. 4
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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