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Antimicrobial Agents and Chemotherapy, May 1999, p. 1301-1303, Vol. 43, No. 5
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Resistance to beta -Lactam Antibiotics in Pseudomonas aeruginosa Due to Interplay between the MexAB-OprM Efflux Pump and beta -Lactamase

Taiji Nakae,* Akira Nakajima, Toshihisa Ono, Kohjiro Saito, and Hiroshi Yoneyama

Department of Molecular Life Science, Tokai University School of Medicine, Isehara 259-1193, Japan

Received 7 December 1998/Returned for modification 8 February 1999/Accepted 3 March 1999

We evaluated the roles of the MexAB-OprM efflux pump and beta -lactamase in beta -lactam resistance in Pseudomonas aeruginosa by constructing OprM-deficient, OprM basal level, and OprM fully expressed mutants from beta -lactamase-negative, -inducible, and -overexpressed strains. We conclude that, with the notable exception of imipenem, the MexAB-OprM pump contributes significantly to beta -lactam resistance in both beta -lactamase-negative and beta -lactamase-inducible strains, while the contribution of the MexAB-OprM efflux system is negligible in strains with overexpressed beta -lactamase. Overexpression of the efflux pump alone contributes to the high level of beta -lactam resistance in the absence of beta -lactamase.

* Corresponding author. Mailing address: Department of Molecular Life Science, Tokai University School of Medicine, Isehara 259-1193, Japan. Phone: 81-465-93-5436. Fax: 81-463-93-5437. E-mail: nakae{at}is.icc.u-tokai.ac.jp.

Antimicrobial Agents and Chemotherapy, May 1999, p. 1301-1303, Vol. 43, No. 5
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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