This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Danel, F.
Right arrow Articles by Livermore, D. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Danel, F.
Right arrow Articles by Livermore, D. M.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, June 1999, p. 1362-1366, Vol. 43, No. 6
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

OXA-17, a Further Extended-Spectrum Variant of OXA-10 beta -Lactamase, Isolated from Pseudomonas aeruginosa

Franck Danel,1,* Lucinda M. C. Hall,1 Brigid Duke,1 Deniz Gur,2 and David M. Livermore1,dagger

Antibiotic Group, Department of Medical Microbiology, St. Bartholomew's and the Royal London School of Medicine and Dentistry, London, E1 2AD, United Kingdom,1 and Section of Infectious Diseases, Department of Internal Medicine, Hacettepe University School of Medicine, 06100 Ankara, Turkey2

Received 16 June 1998/Returned for modification 24 November 1998/Accepted 12 March 1999

Pseudomonas aeruginosa isolates 871 and 873 were isolated at Hacettepe University Hospital in Ankara and were highly resistant to ceftazidime (MIC, 128 µg/ml). Each produced three beta -lactamases, with pIs of 5.3, 6.1, and 7.9. The beta -lactamase with a pI of 5.3 was previously shown to be PER-1 enzyme. The antibiograms of the isolates were not entirely explained by production of PER-1 enzyme, insofar as ceftazidime resistance was incompletely reversed by clavulanate. The enzymes with pIs of 6.1 and 7.9 were therefore investigated. The enzyme with a pI of 6.1 proved to be a novel mutant of OXA-10, which we designated OXA-17, and had asparagine changed to serine at position 73 of the protein. When cloned into Escherichia coli XL1-blue, OXA-17 enzyme conferred greater resistance to cefotaxime, latamoxef, and cefepime than did OXA-10, but it had only a marginal (two- to fourfold) effect on the MIC of ceftazidime. This behavior contrasted with that of previous OXA-10 mutants, specifically OXA-11, -14, and -16, which predominately compromise ceftazidime. Extracted OXA-17 enzyme had relatively greater activity than OXA-10 against oxacillin, cloxacillin, and cefotaxime but, in terms of kcat/Km, it had lower catalytic efficiency against most beta -lactams. The enzyme with a pI of 7.9 was shown by gene sequencing to be OXA-2.

* Corresponding author. Present address: Pharmaceuticals Division, Pharma Research Preclinical Infectious Disease, F. Hoffmann-La Roche Ltd., CH-4070 Basel, Switzerland. Phone: 41-61-6880537. Fax: 41-61-6882729. E-mail: franck.danel{at}roche.com.

dagger Present address: Antibiotic Reference Unit, Laboratory of Hospital Infection, Central Public Health Laboratory, London, NW9 5HT, United Kingdom.

Antimicrobial Agents and Chemotherapy, June 1999, p. 1362-1366, Vol. 43, No. 6
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Strateva, T., Yordanov, D. (2009). Pseudomonas aeruginosa - a phenomenon of bacterial resistance. J Med Microbiol 58: 1133-1148 [Abstract] [Full Text]  
  • Empel, J., Filczak, K., Mrowka, A., Hryniewicz, W., Livermore, D. M., Gniadkowski, M. (2007). Outbreak of Pseudomonas aeruginosa Infections with PER-1 Extended-Spectrum {beta}-Lactamase in Warsaw, Poland: Further Evidence for an International Clonal Complex. J. Clin. Microbiol. 45: 2829-2834 [Abstract] [Full Text]  
  • Yan, J.-J., Hsueh, P.-R., Lu, J.-J., Chang, F.-Y., Ko, W.-C., Wu, J.-J. (2006). Characterization of acquired {beta}-lactamases and their genetic support in multidrug-resistant Pseudomonas aeruginosa isolates in Taiwan: the prevalence of unusual integrons. J Antimicrob Chemother 58: 530-536 [Abstract] [Full Text]  
  • Anderson, D. J., Engemann, J. J., Harrell, L. J., Carmeli, Y., Reller, L. B., Kaye, K. S. (2006). Predictors of Mortality in Patients with Bloodstream Infection Due to Ceftazidime-Resistant Klebsiella pneumoniae.. Antimicrob. Agents Chemother. 50: 1715-1720 [Abstract] [Full Text]  
  • Paterson, D. L., Bonomo, R. A. (2005). Extended-Spectrum {beta}-Lactamases: a Clinical Update. Clin. Microbiol. Rev. 18: 657-686 [Abstract] [Full Text]  
  • Livermore, D. M., Hawkey, P. M. (2005). CTX-M: changing the face of ESBLs in the UK. J Antimicrob Chemother 56: 451-454 [Abstract] [Full Text]  
  • Lee, S., Park, Y.-J., Kim, M., Lee, H. K., Han, K., Kang, C. S., Kang, M. W. (2005). Prevalence of Ambler class A and D {beta}-lactamases among clinical isolates of Pseudomonas aeruginosa in Korea. J Antimicrob Chemother 56: 122-127 [Abstract] [Full Text]  
  • Jacoby, G. A., Munoz-Price, L. S. (2005). The New {beta}-Lactamases. NEJM 352: 380-391 [Full Text]  
  • Niumsup, P., Wuthiekanun, V. (2002). Cloning of the class D {beta}-lactamase gene from Burkholderia pseudomallei and studies on its expression in ceftazidime-susceptible and -resistant strains. J Antimicrob Chemother 50: 445-455 [Abstract] [Full Text]  
  • De Champs, C., Poirel, L., Bonnet, R., Sirot, D., Chanal, C., Sirot, J., Nordmann, P. (2002). Prospective Survey of {beta}-Lactamases Produced by Ceftazidime- Resistant Pseudomonas aeruginosa Isolated in a French Hospital in 2000. Antimicrob. Agents Chemother. 46: 3031-3034 [Abstract] [Full Text]  
  • Bert, F., Branger, C., Lambert-Zechovsky, N. (2002). Identification of PSE and OXA {beta}-lactamase genes in Pseudomonas aeruginosa using PCR-restriction fragment length polymorphism. J Antimicrob Chemother 50: 11-18 [Abstract] [Full Text]  
  • Poirel, L., Naas, T., Le Thomas, I., Karim, A., Bingen, E., Nordmann, P. (2001). CTX-M-Type Extended-Spectrum beta -Lactamase That Hydrolyzes Ceftazidime through a Single Amino Acid Substitution in the Omega Loop. Antimicrob. Agents Chemother. 45: 3355-3361 [Abstract] [Full Text]  
  • Bradford, P. A. (2001). Extended-Spectrum {beta}-Lactamases in the 21st Century: Characterization, Epidemiology, and Detection of This Important Resistance Threat. Clin. Microbiol. Rev. 14: 933-951 [Abstract] [Full Text]  
  • Petrella, S., Clermont, D., Casin, I., Jarlier, V., Sougakoff, W. (2001). Novel Class A {beta}-Lactamase Sed-1 from Citrobacter sedlakii: Genetic Diversity of {beta}-Lactamases within the Citrobacter Genus. Antimicrob. Agents Chemother. 45: 2287-2298 [Abstract] [Full Text]  
  • Aubert, D., Poirel, L., Chevalier, J., Leotard, S., Pages, J.-M., Nordmann, P. (2001). Oxacillinase-Mediated Resistance to Cefepime and Susceptibility to Ceftazidime in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 45: 1615-1620 [Abstract] [Full Text]  
  • Poirel, L., Girlich, D., Naas, T., Nordmann, P. (2001). OXA-28, an Extended-Spectrum Variant of OXA-10 {beta}-Lactamase from Pseudomonas aeruginosa and Its Plasmid- and Integron-Located Gene. Antimicrob. Agents Chemother. 45: 447-453 [Abstract] [Full Text]  
  • Bou, G., Oliver, A., Martínez-Beltrán, J. (2000). OXA-24, a Novel Class D beta -Lactamase with Carbapenemase Activity in an Acinetobacter baumannii Clinical Strain. Antimicrob. Agents Chemother. 44: 1556-1561 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»