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Antimicrobial Agents and Chemotherapy, June 1999, p. 1367-1372, Vol. 43, No. 6
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Erythromycin-Resistant Neisseria gonorrhoeae and Oral Commensal Neisseria spp. Carry Known rRNA Methylase Genes

Marilyn C. Roberts,1,* Whasun O. Chung,1 Darcie Roe,1,dagger Minsheng Xia,1,Dagger Carolina Marquez,2 G. Borthagaray,2 William L. Whittington,3 and King K. Holmes3

Departments of Pathobiology1 and Medicine,3 University of Washington, Seattle, Washington 98195, and Catedra de Microbiologia, Facultad de Quimica, Montevideo, Uruguay2

Received 1 October 1998/Returned for modification 20 January 1999/Accepted 12 March 1999

Two Neisseria gonorrhoeae isolates from Seattle and two isolates from Uruguay were resistant to erythromycin (MIC, 4 to 16 µg/ml) and had reduced susceptibility to azithromycin (MIC, 1 to 4 µg/ml) due to the presence of the self-mobile rRNA methylase gene(s) ermF or ermB and ermF. The two Seattle isolates and one isolate from Uruguay were multiresistant, carrying either the 25.2-MDa tetM-containing plasmid (Seattle) or a beta -lactamase plasmid (Uruguay). Sixteen commensal Neisseria isolates (10 Neisseria perflava-N. sicca, 2 N. flava, and 4 N. mucosa) for which erythromycin MICs were 4 to 16 µg/ml were shown to carry one or more known rRNA methylase genes, including ermB, ermC, and/or ermF. Many of these isolates also were multiresistant and carried the tetM gene. This is the first time that a complete transposon or a complete conjugative transposon carrying an antibiotic resistance gene has been described for the genus Neisseria.

* Corresponding author. Mailing address: Department of Pathobiology, Box 357238, University of Washington, Seattle, WA 98195-7238. Phone: (206) 543-8001. Fax: (206) 543-3873. E-mail: marilynr{at}u.washington.edu.

dagger Present address: Dade MicroScan Inc., West Sacramento, CA 95691.

Dagger Present address: Department of Medicine, University of Washington, Seattle, WA 98195.

Antimicrobial Agents and Chemotherapy, June 1999, p. 1367-1372, Vol. 43, No. 6
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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