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Antimicrobial Agents and Chemotherapy, June 1999, p. 1500-1502, Vol. 43, No. 6
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Interstrain Variation in the Human Cytomegalovirus DNA Polymerase Sequence and Its Effect on Genotypic Diagnosis of Antiviral Drug Resistance

Sunwen Chou,1,* Nell S. Lurain,2 Adriana Weinberg,3 Guang-Yung Cai,3 Prem L. Sharma,4 Clyde S. Crumpacker,4 and Adult Aids Clinical Trials Group CMV Laboratories

VA Medical Center, Portland, Oregon1; Rush Medical Center, Chicago, Illinois2; University of Colorado Health Sciences Center, Denver, Colorado3; and Beth-Israel Deaconess Medical Center, Boston, Massachusetts4

Received 28 October 1998/Returned for modification 20 January 1999/Accepted 30 March 1999

The polymerase (pol) coding sequence was determined for 40 independent clinical cytomegalovirus isolates sensitive to ganciclovir and foscarnet. Sequence alignments showed >98% interstrain homology and amino acid variation in only 4% of the 1,237 codons. Almost all variation occurred outside of conserved functional domains where resistance mutations have been identified.

* Corresponding author. Mailing address: Infectious Disease Section P3ID, VA Medical Center, 3710 S.W. U.S. Veterans Hospital Rd., Portland, OR 97201. Phone: (503) 273-5185. Fax: (503) 273-5348. E-mail: chous{at}ohsu.edu.

Antimicrobial Agents and Chemotherapy, June 1999, p. 1500-1502, Vol. 43, No. 6
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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