This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Komatsuzawa, H.
Right arrow Articles by Suginaka, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Komatsuzawa, H.
Right arrow Articles by Suginaka, H.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, July 1999, p. 1578-1583, Vol. 43, No. 7
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Cloning and Characterization of a Gene, pbpF, Encoding a New Penicillin-Binding Protein, PBP2B, in Staphylococcus aureus

Hitoshi Komatsuzawa,1,* Gil H. Choi,2 Kouji Ohta,1 Motoyuki Sugai,1 Monique T. Tran,2 and Hidekazu Suginaka1

Department of Microbiology, Hiroshima University School of Dentistry, Kasumi 1-2-3, Minami-ku, Hiroshima City, Hiroshima 734-8553, Japan,1 and Human Genome Sciences, Inc., Rockville, Maryland 208502

Received 28 December 1998/Returned for modification 25 January 1999/Accepted 12 April 1999

A previously unrecognized penicillin binding protein (PBP) gene, pbpF, was identified in Staphylococcus aureus. This gene encodes a protein of 691 amino acid residues with an estimated molecular mass of 78 kDa. The molecular mass is very close to that of S. aureus PBP2 (81 kDa), and the protein is tentatively named PBP2B. PBP2B has three motifs, SSVK, SSN, and KTG, that can be found in PBPs and beta -lactamases. Recombinant PBP2B (rPBP2B), which lacks a putative signal peptide at the N terminus and has a histidine tag at the C terminus, was expressed in Escherichia coli. The purified rPBP2B was shown to have penicillin binding activity. A protein band was detected from S. aureus membrane fraction by immunoblotting with anti-rPBP2B serum. Also, penicillin binding activity of the protein immunoprecipitated with anti-rPBP2B serum was detected. These results suggest the presence of PBP2B in S. aureus cell membrane that covalently binds penicillin. The internal region of pbpF and PBP2B protein were found in all 12 S. aureus strains tested by PCR and immunoblotting.

* Corresponding author. Mailing address: Department of Microbiology, Hiroshima University School of Dentistry, Kasumi 1-2-3, Minami-ku, Hiroshima City, Hiroshima 734-8553, Japan. Phone: 81 82 257 5636. Fax: 81 82 257 5639. E-mail: hkomatsu{at}ipc.hiroshima-u.ac.jp.

Antimicrobial Agents and Chemotherapy, July 1999, p. 1578-1583, Vol. 43, No. 7
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Baudoux, P., Bles, N., Lemaire, S., Mingeot-Leclercq, M.-P., Tulkens, P. M., Van Bambeke, F. (2007). Combined effect of pH and concentration on the activities of gentamicin and oxacillin against Staphylococcus aureus in pharmacodynamic models of extracellular and intracellular infections. J Antimicrob Chemother 59: 246-253 [Abstract] [Full Text]  
  • Miller, K., Storey, C., Stubbings, W. J., Hoyle, A. M., Hobbs, J. K., Chopra, I. (2005). Antistaphylococcal activity of the novel cephalosporin CB-181963 (CAB-175). J Antimicrob Chemother 55: 579-582 [Abstract] [Full Text]  
  • Boyle-Vavra, S., Yin, S., Challapalli, M., Daum, R. S. (2003). Transcriptional Induction of the Penicillin-Binding Protein 2 Gene in Staphylococcus aureus by Cell Wall-Active Antibiotics Oxacillin and Vancomycin. Antimicrob. Agents Chemother. 47: 1028-1036 [Abstract] [Full Text]  
  • Komatsuzawa, H., Ohta, K., Yamada, S., Ehlert, K., Labischinski, H., Kajimura, J., Fujiwara, T., Sugai, M. (2002). Increased Glycan Chain Length Distribution and Decreased Susceptibility to Moenomycin in a Vancomycin-Resistant Staphylococcus aureus Mutant. Antimicrob. Agents Chemother. 46: 75-81 [Abstract] [Full Text]  
  • Pinho, M. G., de Lencastre, H., Tomasz, A. (2000). Cloning, Characterization, and Inactivation of the Gene pbpC, Encoding Penicillin-Binding Protein 3 of Staphylococcus aureus. J. Bacteriol. 182: 1074-1079 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»