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Antimicrobial Agents and Chemotherapy, July 1999, p. 1704-1707, Vol. 43, No. 7
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

In Vitro Antifungal Activity and Cytotoxicity of a Novel Membrane-Active Peptide

Sung Yu Hong, Jong Eun Oh, and Keun Hyeung Lee*

Protein Chemistry Laboratory, Mogam Biotechnology Research Institute, Kyunggi-Do, 449-910, Korea

Received 7 December 1998/Returned for modification 27 January 1999/Accepted 23 April 1999

In the present study, we investigated the antifungal activity and cytotoxicity of a novel membrane-active peptide, KKVVFKVKFKK (MP). MP inhibited the growth of various pathogenic fungi isolated from patients and of fluconazole-resistant fungi at concentrations of 2 to 32 µg/ml. MP had potent fungicidal activity; the minimal fungicidal concentrations of the peptide were no more than fourfold greater than the MICs. Time course experiments of MP-induced killing of Candida albicans ATCC 36232 showed that the rate of killing was rapid and depended on the concentration of MP. MP had a strong synergism with other antifungal drugs; the fractional inhibitory concentration index values of MP with amphotericin B and fluconazole for C. albicans were 0.16 and 0.02, respectively. The 50% inhibitory concentrations of MP for NIH 3T3 and Jurkat cells were approximately 100 times higher than the MIC for C. albicans ATCC 36232, indicating that MP had high selectivity between the fungal and mammalian cells. These results suggest that MP has great advantages in the development of antifungal agents.

* Corresponding author. Mailing address: Protein Chemistry Laboratory, Mogam Biotechnology Research Institute, 341 Pojung-Ri, Koosung-Myun, Youngin City, Kyunggi-Do, 449-910, Korea. Phone: 82-331-262-3851. Fax: 82-331-262-6622. E-mail: lkh{at}kgcc.co.kr.

Antimicrobial Agents and Chemotherapy, July 1999, p. 1704-1707, Vol. 43, No. 7
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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