This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bauernfeind, A.
Right arrow Articles by Ullmann, U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bauernfeind, A.
Right arrow Articles by Ullmann, U.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, August 1999, p. 1924-1931, Vol. 43, No. 8
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A Novel Type of AmpC beta -Lactamase, ACC-1, Produced by a Klebsiella pneumoniae Strain Causing Nosocomial Pneumonia

Adolf Bauernfeind,1,* Ines Schneider,1 Renate Jungwirth,1 Hany Sahly,2 and Uwe Ullmann2

Max von Pettenkofer Institute, Munich,1 and Institute for Medical Microbiology and Virology, Kiel,2 Federal Republic of Germany

Received 16 October 1998/Returned for modification 15 February 1999/Accepted 7 June 1999

A Klebsiella pneumoniae strain resistant to oxyimino cephalosporins was cultured from respiratory secretions of a patient suffering from nosocomial pneumonia in Kiel, Germany, in 1997. The isolate harbors a bla resistance gene located on a transmissible plasmid. An Escherichia coli transconjugant produces a beta -lactamase with an isoelectric point of 7.7 and a resistance phenotype characteristic of an AmpC (class 1) beta -lactamase except for low MICs of cephamycins. The bla gene was cloned and sequenced. It encodes a protein of 386 amino acids with the active site serine of the S-X-X-K motif at position 64, as is characteristic for class C beta -lactamases. Multiple alignment of the deduced amino acid sequence with 21 other AmpC beta -lactamases demonstrates only very distant homology, reaching at maximum 52.3% identity for the chromosomal AmpC beta -lactamase of Serratia marcescens SR50. The beta -lactamase of K. pneumoniae KUS represents a new type of AmpC-class enzyme, for which we propose the designation ACC-1 (Ambler class C-1).

* Corresponding author. Mailing address: Max von Pettenkofer-Institut, Pettenkoferstr. 9a, 80336 Munich, Germany. Phone: 0049-89-5160 5268. Fax: 0049-89-5160 5266. E-mail: Adolf.Bauernfeind{at}mvp-bak.med.uni-muenchen.de.

Antimicrobial Agents and Chemotherapy, August 1999, p. 1924-1931, Vol. 43, No. 8
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Schneider, I., Queenan, A. M., Markovska, R., Markova, B., Keuleyan, E., Bauernfeind, A. (2009). New Variant of CTX-M-Type Extended-Spectrum {beta}-Lactamases, CTX-M-71, with a Gly238Cys Substitution in a Klebsiella pneumoniae Isolate from Bulgaria. Antimicrob. Agents Chemother. 53: 4518-4521 [Abstract] [Full Text]  
  • Jacoby, G. A. (2009). AmpC {beta}-Lactamases. Clin. Microbiol. Rev. 22: 161-182 [Abstract] [Full Text]  
  • Haldorsen, B., Aasnaes, B., Dahl, K. H., Hanssen, A.-M., Simonsen, G. S., Walsh, T. R., Sundsfjord, A., Lundblad, E. W. (2008). The AmpC phenotype in Norwegian clinical isolates of Escherichia coli is associated with an acquired ISEcp1-like ampC element or hyperproduction of the endogenous AmpC. J Antimicrob Chemother 62: 694-702 [Abstract] [Full Text]  
  • Gonullu, N., Aktas, Z., Kayacan, C. B., Salcioglu, M., Carattoli, A., Yong, D. E., Walsh, T. R. (2008). Dissemination of CTX-M-15 {beta}-Lactamase Genes Carried on Inc FI and FII Plasmids among Clinical Isolates of Escherichia coli in a University Hospital in Istanbul, Turkey. J. Clin. Microbiol. 46: 1110-1112 [Abstract] [Full Text]  
  • Vimont, S., Aubert, D., Mazoit, J.-X., Poirel, L., Nordmann, P. (2007). Broad-spectrum {beta}-lactams for treating experimental peritonitis in mice due to Escherichia coli producing plasmid-encoded cephalosporinases. J Antimicrob Chemother 60: 1045-1050 [Abstract] [Full Text]  
  • Papagiannitsis, C. C., Tzouvelekis, L. S., Tzelepi, E., Miriagou, V. (2007). Plasmid-Encoded ACC-4, an Extended-Spectrum Cephalosporinase Variant from Escherichia coli. Antimicrob. Agents Chemother. 51: 3763-3767 [Abstract] [Full Text]  
  • Papagiannitsis, C. C., Loli, A., Tzouvelekis, L. S., Tzelepi, E., Arlet, G., Miriagou, V. (2007). SCO-1, a Novel Plasmid-Mediated Class A {beta}-Lactamase with Carbenicillinase Characteristics from Escherichia coli. Antimicrob. Agents Chemother. 51: 2185-2188 [Abstract] [Full Text]  
  • Doloy, A., Verdet, C., Gautier, V., Decre, D., Ronco, E., Hammami, A., Philippon, A., Arlet, G. (2006). Genetic Environment of Acquired blaACC-1 {beta}-Lactamase Gene in Enterobacteriaceae Isolates. Antimicrob. Agents Chemother. 50: 4177-4181 [Abstract] [Full Text]  
  • Ruppe, E., Bidet, P., Verdet, C., Arlet, G., Bingen, E. (2006). First Detection of the Ambler Class C 1 AmpC {beta}-Lactamase in Citrobacter freundii by a New, Simple Double-Disk Synergy Test. J. Clin. Microbiol. 44: 4204-4207 [Abstract] [Full Text]  
  • Jacoby, G. A., Walsh, K. E., Walker, V. J. (2006). Identification of Extended-Spectrum, AmpC, and Carbapenem- Hydrolyzing {beta}-Lactamases in Escherichia coli and Klebsiella pneumoniae by Disk Tests.. J. Clin. Microbiol. 44: 1971-1976 [Abstract] [Full Text]  
  • Jacoby, G. A. (2006). {beta}-Lactamase Nomenclature.. Antimicrob. Agents Chemother. 50: 1123-1129 [Full Text]  
  • Lartigue, M.-F., Poirel, L., Aubert, D., Nordmann, P. (2006). In Vitro Analysis of ISEcp1B-Mediated Mobilization of Naturally Occurring {beta}-Lactamase Gene blaCTX-M of Kluyvera ascorbata.. Antimicrob. Agents Chemother. 50: 1282-1286 [Abstract] [Full Text]  
  • Schneider, I., Queenan, A. M., Bauernfeind, A. (2006). Novel Carbapenem-Hydrolyzing Oxacillinase OXA-62 from Pandoraea pnomenusa.. Antimicrob. Agents Chemother. 50: 1330-1335 [Abstract] [Full Text]  
  • Bidet, P., Burghoffer, B., Gautier, V., Brahimi, N., Mariani-Kurkdjian, P., El-Ghoneimi, A., Bingen, E., Arlet, G. (2005). In Vivo Transfer of Plasmid-Encoded ACC-1 AmpC from Klebsiella pneumoniae to Escherichia coli in an Infant and Selection of Impermeability to Imipenem in K. pneumoniae. Antimicrob. Agents Chemother. 49: 3562-3565 [Abstract] [Full Text]  
  • Coudron, P. E. (2005). Inhibitor-Based Methods for Detection of Plasmid-Mediated AmpC {beta}-Lactamases in Klebsiella spp., Escherichia coli, and Proteus mirabilis. J. Clin. Microbiol. 43: 4163-4167 [Abstract] [Full Text]  
  • Hasman, H., Mevius, D., Veldman, K., Olesen, I., Aarestrup, F. M. (2005). {beta}-Lactamases among extended-spectrum {beta}-lactamase (ESBL)-resistant Salmonella from poultry, poultry products and human patients in The Netherlands. J Antimicrob Chemother 56: 115-121 [Abstract] [Full Text]  
  • Ohana, S., Leflon, V., Ronco, E., Rottman, M., Guillemot, D., Lortat-Jacob, S., Denys, P., Loubert, G., Nicolas-Chanoine, M.-H., Gaillard, J.-L., Lawrence, C. (2005). Spread of a Klebsiella pneumoniae Strain Producing a Plasmid- Mediated ACC-1 AmpC {beta}-Lactamase in a Teaching Hospital Admitting Disabled Patients. Antimicrob. Agents Chemother. 49: 2095-2097 [Abstract] [Full Text]  
  • Miro, E., Mirelis, B., Navarro, F., Matas, L., Gimenez, M., Rabaza, C. (2005). Escherichia coli Producing an ACC-1 Class C {beta}-Lactamase Isolated in Barcelona, Spain. Antimicrob. Agents Chemother. 49: 866-867 [Full Text]  
  • Poirel, L., Lartigue, M.-F., Decousser, J.-W., Nordmann, P. (2005). ISEcp1B-Mediated Transposition of blaCTX-M in Escherichia coli. Antimicrob. Agents Chemother. 49: 447-450 [Abstract] [Full Text]  
  • Poirel, L., Decousser, J.-W., Nordmann, P. (2003). Insertion Sequence ISEcp1B Is Involved in Expression and Mobilization of a blaCTX-M {beta}-Lactamase Gene. Antimicrob. Agents Chemother. 47: 2938-2945 [Abstract] [Full Text]  
  • Coudron, P. E., Hanson, N. D., Climo, M. W. (2003). Occurrence of Extended-Spectrum and AmpC Beta-Lactamases in Bloodstream Isolates of Klebsiella pneumoniae: Isolates Harbor Plasmid-Mediated FOX-5 and ACT-1 AmpC Beta-Lactamases. J. Clin. Microbiol. 41: 772-777 [Abstract] [Full Text]  
  • Manchanda, V., Singh, N. P. (2003). Occurrence and detection of AmpC {beta}-lactamases among Gram-negative clinical isolates using a modified three-dimensional test at Guru Tegh Bahadur Hospital, Delhi, India. J Antimicrob Chemother 51: 415-418 [Abstract] [Full Text]  
  • Yan, J.-J., Ko, W.-C., Jung, Y.-C., Chuang, C.-L., Wu, J.-J. (2002). Emergence of Klebsiella pneumoniae Isolates Producing Inducible DHA-1 {beta}-Lactamase in a University Hospital in Taiwan. J. Clin. Microbiol. 40: 3121-3126 [Abstract] [Full Text]  
  • Vakulenko, S. B., Golemi, D., Geryk, B., Suvorov, M., Knox, J. R., Mobashery, S., Lerner, S. A. (2002). Mutational Replacement of Leu-293 in the Class C Enterobacter cloacae P99 {beta}-Lactamase Confers Increased MIC of Cefepime. Antimicrob. Agents Chemother. 46: 1966-1970 [Abstract] [Full Text]  
  • Philippon, A., Arlet, G., Jacoby, G. A. (2002). Plasmid-Determined AmpC-Type {beta}-Lactamases. Antimicrob. Agents Chemother. 46: 1-11 [Full Text]  
  • Zhang, Z., Yu, Y., Musser, J. M., Palzkill, T. (2001). Amino Acid Sequence Determinants of Extended Spectrum Cephalosporin Hydrolysis by the Class C P99 beta -Lactamase. J. Biol. Chem. 276: 46568-46574 [Abstract] [Full Text]  
  • Queenan, A. M., Jenkins, S., Bush, K. (2001). Cloning and Biochemical Characterization of FOX-5, an AmpC-Type Plasmid-Encoded beta -Lactamase from a New York City Klebsiella pneumoniae Clinical Isolate. Antimicrob. Agents Chemother. 45: 3189-3194 [Abstract] [Full Text]  
  • Navarro, F., Perez-Trallero, E., Marimon, J. M., Aliaga, R., Gomariz, M., Mirelis, B. (2001). CMY-2-producing Salmonella enterica, Klebsiella pneumoniae, Klebsiella oxytoca, Proteus mirabilis and Escherichia coli strains isolated in Spain (October 1999-December 2000). J Antimicrob Chemother 48: 383-389 [Abstract] [Full Text]  
  • Wu, T.-L., Siu, L. K., Su, L.-H., Lauderdale, T. L., Lin, F. M., Leu, H.-S., Lin, T.-Y., Ho, M. (2001). Outer membrane protein change combined with co-existing TEM-1 and SHV-1 {beta}-lactamases lead to false identification of ESBL-producing Klebsiella pneumoniae. J Antimicrob Chemother 47: 755-761 [Abstract] [Full Text]  
  • Fortineau, N., Poirel, L., Nordmann, P. (2001). Plasmid-mediated and inducible cephalosporinase DHA-2 from Klebsiella pneumoniae. J Antimicrob Chemother 47: 207-210 [Abstract] [Full Text]  
  • Girlich, D., Naas, T., Bellais, S., Poirel, L., Karim, A., Nordmann, P. (2000). Heterogeneity of AmpC Cephalosporinases of Hafnia alvei Clinical Isolates Expressing Inducible or Constitutive Ceftazidime Resistance Phenotypes. Antimicrob. Agents Chemother. 44: 3220-3223 [Abstract] [Full Text]  
  • Girlich, D., Naas, T., Bellais, S., Poirel, L., Karim, A., Nordmann, P. (2000). Biochemical-Genetic Characterization and Regulation of Expression of an ACC-1-Like Chromosome-Borne Cephalosporinase from Hafnia alvei. Antimicrob. Agents Chemother. 44: 1470-1478 [Abstract] [Full Text]  
  • Coudron, P. E., Moland, E. S., Thomson, K. S. (2000). Occurrence and Detection of AmpC Beta-Lactamases among Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis Isolates at a Veterans Medical Center. J. Clin. Microbiol. 38: 1791-1796 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»